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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
14/05/2010 |
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Data da última atualização: |
18/05/2010 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GUIMARÃES, A. P.; DINIZ, A.; CALDEIRA, E.; FAÇANHA, A.; OLIVARES, F. L.; HUERGO, L.; PEDROSA, F. O.; BODDEY, R. M. |
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Afiliação: |
Ana Paula Guimarães, UFRRJ; Anderson Diniz, UFRRJ; Elisamara Caldeira, UFRRJ; Arnoldo Façanha, UENF; Fábio L. Olivares, UENF; Luciano Huergo, UFPR; Fábio O. Pedrosa, UFPR; ROBERT MICHAEL BODDEY, CNPAB. |
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Título: |
Aprimoramento da técnica purificação da nitrogenase para quantificação da FBN por abundância natural de 15N. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações. Seropédica: Embrapa Agrobiologia, 2009. |
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Idioma: |
Português |
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Palavras-Chave: |
Fracionamento isotópico de 15N. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00760naa a2200205 a 4500
001 1853035
005 2010-05-18
008 2009 bl --- 0-- u #d
100 1 $aGUIMARÃES, A. P.
245 $aAprimoramento da técnica purificação da nitrogenase para quantificação da FBN por abundância natural de 15N.
260 $c2009
653 $aFracionamento isotópico de 15N
700 1 $aDINIZ, A.
700 1 $aCALDEIRA, E.
700 1 $aFAÇANHA, A.
700 1 $aOLIVARES, F. L.
700 1 $aHUERGO, L.
700 1 $aPEDROSA, F. O.
700 1 $aBODDEY, R. M.
773 $tIn: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações. Seropédica: Embrapa Agrobiologia, 2009.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
23/11/2017 |
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Data da última atualização: |
23/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
PAIVA, P. P. de; DELCORSO, M. C.; MATHEUS, V. A.; QUEIROZ, S. C. do N. de; COLLARES-BUZATO, C. B.; ARANA, S. |
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Afiliação: |
PAULA PEREIRA DE PAIVA, CPQBA-UNICAMP; MARIANA CRUZ DELCORSO, CPQBA-UNICAMP; VALQUIRIA APARECIDA MATHEUS, CPQBA-UNICAMP; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; SARAH ARANA, CPQBA-UNICAMP. |
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Título: |
Acute toxicity of commercial atrazine in Piaractus mesopotamicus: histopathological, ultrastructural, molecular, and genotoxic evaluation. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Veterinary World, Wankaner, v. 10, n. 9, p. 1008-1019, 2017. |
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DOI: |
http://dx.doi.org/10.14202/vetworld.2017.1008-1019 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this work was to evaluate the sensitivity of Pacu fingerlings (Piaractus mesopotamicus) by measuring the effects of median lethal concentration (LC50) of atrazine (ATZ - 28.58 mg/L) after acute exposure (up to 96 h). The fish were exposed to the LC50 of ATZ for 96 h (28.58 mg/L) in a static system. During the experiment, the fingerlings were randomly distributed in four glass tanks (50 L) containing dechlorinated water. Four glass tanks were for the control group, and four were for the ATZ-exposed group (n=4 per glass tank), given a total number of 16 animals tested per group. The genotoxicity was evaluated by micronucleus (MN) test in erythrocytes from peripheral blood. Qualitative and semi-quantitative histopathological analyses, and also ultrastructural study, were applied in liver and kidney samples. Finally, the content of heat shock protein (Hsp70) in the liver was evaluated by the western blotting method. The morphological alterations in the liver, which was associated with increased expression of Hsp70, included nuclear and cytoplasmic vacuolization, cytoplasmic hyaline inclusions, and necrosis. The kidney presented edema and tubular cell degeneration with cytoplasmic hyaline inclusion. The semi-quantitative histopathological analyses indicated that the liver was more sensitive than kidney to ATZ-induced damage. Ultrastructural analysis showed that ATZ caused membrane alterations in several organelles and increased the number of lysosomes in hepatocytes and kidney proximal tubular cells. Nevertheless, no significant difference was observed in MN frequency in erythrocytes comparing treated and control groups. These results indicated that ATZ-induced damage to the kidney and liver function, ATZ at the concentration tested did not induce a significant difference in MN frequency in Pacu erythrocytes comparing treated and control groups, and also that Pacu fingerlings may be a good bioindicator for testing freshwater contamination. MenosThe aim of this work was to evaluate the sensitivity of Pacu fingerlings (Piaractus mesopotamicus) by measuring the effects of median lethal concentration (LC50) of atrazine (ATZ - 28.58 mg/L) after acute exposure (up to 96 h). The fish were exposed to the LC50 of ATZ for 96 h (28.58 mg/L) in a static system. During the experiment, the fingerlings were randomly distributed in four glass tanks (50 L) containing dechlorinated water. Four glass tanks were for the control group, and four were for the ATZ-exposed group (n=4 per glass tank), given a total number of 16 animals tested per group. The genotoxicity was evaluated by micronucleus (MN) test in erythrocytes from peripheral blood. Qualitative and semi-quantitative histopathological analyses, and also ultrastructural study, were applied in liver and kidney samples. Finally, the content of heat shock protein (Hsp70) in the liver was evaluated by the western blotting method. The morphological alterations in the liver, which was associated with increased expression of Hsp70, included nuclear and cytoplasmic vacuolization, cytoplasmic hyaline inclusions, and necrosis. The kidney presented edema and tubular cell degeneration with cytoplasmic hyaline inclusion. The semi-quantitative histopathological analyses indicated that the liver was more sensitive than kidney to ATZ-induced damage. Ultrastructural analysis showed that ATZ caused membrane alterations in several organelles and increased the number of lysosomes in hepatocytes an... Mostrar Tudo |
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Palavras-Chave: |
Brazilian ichthyofauna; Kidney lesions; Liver lesions; Micronucleus test. |
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Thesagro: |
Herbicida; Pacu; Poluição da água; Toxidez. |
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Thesaurus NAL: |
Atrazine; Hepatotoxicity; Nephrotoxicity; Oxidative stress; Piaractus mesopotamicus; Water pollution. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/167350/1/2017AP19.pdf
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Marc: |
LEADER 03099naa a2200361 a 4500
001 2080546
005 2017-11-23
008 2017 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.14202/vetworld.2017.1008-1019$2DOI
100 1 $aPAIVA, P. P. de
245 $aAcute toxicity of commercial atrazine in Piaractus mesopotamicus$bhistopathological, ultrastructural, molecular, and genotoxic evaluation.$h[electronic resource]
260 $c2017
520 $aThe aim of this work was to evaluate the sensitivity of Pacu fingerlings (Piaractus mesopotamicus) by measuring the effects of median lethal concentration (LC50) of atrazine (ATZ - 28.58 mg/L) after acute exposure (up to 96 h). The fish were exposed to the LC50 of ATZ for 96 h (28.58 mg/L) in a static system. During the experiment, the fingerlings were randomly distributed in four glass tanks (50 L) containing dechlorinated water. Four glass tanks were for the control group, and four were for the ATZ-exposed group (n=4 per glass tank), given a total number of 16 animals tested per group. The genotoxicity was evaluated by micronucleus (MN) test in erythrocytes from peripheral blood. Qualitative and semi-quantitative histopathological analyses, and also ultrastructural study, were applied in liver and kidney samples. Finally, the content of heat shock protein (Hsp70) in the liver was evaluated by the western blotting method. The morphological alterations in the liver, which was associated with increased expression of Hsp70, included nuclear and cytoplasmic vacuolization, cytoplasmic hyaline inclusions, and necrosis. The kidney presented edema and tubular cell degeneration with cytoplasmic hyaline inclusion. The semi-quantitative histopathological analyses indicated that the liver was more sensitive than kidney to ATZ-induced damage. Ultrastructural analysis showed that ATZ caused membrane alterations in several organelles and increased the number of lysosomes in hepatocytes and kidney proximal tubular cells. Nevertheless, no significant difference was observed in MN frequency in erythrocytes comparing treated and control groups. These results indicated that ATZ-induced damage to the kidney and liver function, ATZ at the concentration tested did not induce a significant difference in MN frequency in Pacu erythrocytes comparing treated and control groups, and also that Pacu fingerlings may be a good bioindicator for testing freshwater contamination.
650 $aAtrazine
650 $aHepatotoxicity
650 $aNephrotoxicity
650 $aOxidative stress
650 $aPiaractus mesopotamicus
650 $aWater pollution
650 $aHerbicida
650 $aPacu
650 $aPoluição da água
650 $aToxidez
653 $aBrazilian ichthyofauna
653 $aKidney lesions
653 $aLiver lesions
653 $aMicronucleus test
700 1 $aDELCORSO, M. C.
700 1 $aMATHEUS, V. A.
700 1 $aQUEIROZ, S. C. do N. de
700 1 $aCOLLARES-BUZATO, C. B.
700 1 $aARANA, S.
773 $tVeterinary World, Wankaner$gv. 10, n. 9, p. 1008-1019, 2017.
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