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| Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
17/03/2011 |
Data da última atualização: |
28/10/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SILVESTRINI, M.; RAMIRO, D. A.; GUERREIRO-FILHO, O.; MALUF, M. P. |
Afiliação: |
Coffee Center "Alcides Carvalho"; IAC; IAC; MIRIAN PEREZ MALUF, SAPC. |
Título: |
Defense-related gene expression in response to leaf-miner infection. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas. |
Idioma: |
Inglês |
Conteúdo: |
In Brazilian coffee plantations, the leaf-miner (Leucoptera coffeella) represents a major threat. Insect control is mainly based on the use of pesticides, as resistant cultivars are not yet available. However, the coffee breeding program of IAC has already selected promising genotypes bearing resistance to the leaf-miner, which was transferred from the diploid species Coffea racemosa. In order to characterize molecular aspects of defense mechanisms associated to the resistance response, expression of key genes was evaluated in coffee leaves, from both resistant and susceptible plants, in response to leaf-miner infection. Infected leavesb were collected at different time-intervals during insect development. Defense-related genes were identified in the Coffee Genome Database, through Blast searches. Gene specific primers were used to amplify corresponding transcripts on sampled leaves using a quantitative RT-PCR approach. Results indicated that there are no significant differences in the expression patterns of evaluated genes when comparing resistant and susceptible infected leaves. Major differences were observed for lipoxygenase, glutathione transferase, protein-kinase receptor and glucanase. However, these differences are mainly associated with expression timing along insect infection rather than with gene regulation, suggesting that resistance to leafminer in coffee may be associated with a previously built basal defense-response. |
Thesagro: |
Leucoptera Coffeella. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01958nam a2200157 a 4500 001 1881314 005 2011-10-28 008 2008 bl uuuu u00u1 u #d 100 1 $aSILVESTRINI, M. 245 $aDefense-related gene expression in response to leaf-miner infection.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.$c2008 520 $aIn Brazilian coffee plantations, the leaf-miner (Leucoptera coffeella) represents a major threat. Insect control is mainly based on the use of pesticides, as resistant cultivars are not yet available. However, the coffee breeding program of IAC has already selected promising genotypes bearing resistance to the leaf-miner, which was transferred from the diploid species Coffea racemosa. In order to characterize molecular aspects of defense mechanisms associated to the resistance response, expression of key genes was evaluated in coffee leaves, from both resistant and susceptible plants, in response to leaf-miner infection. Infected leavesb were collected at different time-intervals during insect development. Defense-related genes were identified in the Coffee Genome Database, through Blast searches. Gene specific primers were used to amplify corresponding transcripts on sampled leaves using a quantitative RT-PCR approach. Results indicated that there are no significant differences in the expression patterns of evaluated genes when comparing resistant and susceptible infected leaves. Major differences were observed for lipoxygenase, glutathione transferase, protein-kinase receptor and glucanase. However, these differences are mainly associated with expression timing along insect infection rather than with gene regulation, suggesting that resistance to leafminer in coffee may be associated with a previously built basal defense-response. 650 $aLeucoptera Coffeella 700 1 $aRAMIRO, D. A. 700 1 $aGUERREIRO-FILHO, O. 700 1 $aMALUF, M. P.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Meio-Norte. Para informações adicionais entre em contato com cpamn.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Meio-Norte. |
Data corrente: |
10/04/2001 |
Data da última atualização: |
28/07/2023 |
Autoria: |
COSTA, N. de L.; TOWNSEND, C. R.; MAGALHAES, J. A.; PEREIRA, R. G. de A. |
Título: |
Resposta de pastagens degradadas de Brachiaria brizantha cv. Marandu a doses de nitrogenio e fosforo. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 37., 2000. Vicosa, MG. Resumos... Vicosa, MG: SBZ, 2000. p. 131 |
Idioma: |
Português |
Conteúdo: |
Avaliou-se o efeito de niveis de nitrogenio (0, 50 e 100kg/ha) e de fosforo (0, 50 e 100kg P2O5/ha) sobre a producao de materia seca (MS) e composicao quimica da forragem de pastagens degradadas de Brachiaria brizantha cv. Marandu. |
Palavras-Chave: |
Braquiaria; Pastagem degradada. |
Thesagro: |
Cálcio; Fósforo; Magnésio; Matéria Seca; Potássio; Proteína Bruta. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 01012nam a2200241 a 4500 001 1060396 005 2023-07-28 008 2000 bl uuuu u00u1 u #d 100 1 $aCOSTA, N. de L. 245 $aResposta de pastagens degradadas de Brachiaria brizantha cv. Marandu a doses de nitrogenio e fosforo.$h[electronic resource] 260 $aIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 37., 2000. Vicosa, MG. Resumos... Vicosa, MG: SBZ, 2000. p. 131$c2000 520 $aAvaliou-se o efeito de niveis de nitrogenio (0, 50 e 100kg/ha) e de fosforo (0, 50 e 100kg P2O5/ha) sobre a producao de materia seca (MS) e composicao quimica da forragem de pastagens degradadas de Brachiaria brizantha cv. Marandu. 650 $aCálcio 650 $aFósforo 650 $aMagnésio 650 $aMatéria Seca 650 $aPotássio 650 $aProteína Bruta 653 $aBraquiaria 653 $aPastagem degradada 700 1 $aTOWNSEND, C. R. 700 1 $aMAGALHAES, J. A. 700 1 $aPEREIRA, R. G. de A.
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