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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/11/2021 |
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Data da última atualização: |
30/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUERRA, P. A.; SOUZA, E. H. de; ANDRADE, E. C. de; MAX, D. de A. S.; OLIVEIRA, R. S.; SOUZA, F. V. D. |
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Afiliação: |
PATRÍCIA ARAÚJO GUERRA, UFRB; EVERTON HILO DE SOUZA, UFRB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; DANIELA DE ANDRADE SILVA MAX, UFRB; RAFAELLE SOUZA DE OLIVEIRA, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Comparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, 2020. |
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ISSN: |
1475-2689 |
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Idioma: |
Inglês |
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Conteúdo: |
The Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed with virus-free plants. MenosThe Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed wi... Mostrar Tudo |
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Thesagro: |
Abacaxi; Planta Ornamental. |
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Categoria do assunto: |
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Marc: |
LEADER 02208naa a2200217 a 4500
001 2136827
005 2021-11-30
008 2020 bl uuuu u00u1 u #d
022 $a1475-2689
100 1 $aGUERRA, P. A.
245 $aComparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties$h[electronic resource]
260 $c2020
520 $aThe Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed with virus-free plants.
650 $aAbacaxi
650 $aPlanta Ornamental
700 1 $aSOUZA, E. H. de
700 1 $aANDRADE, E. C. de
700 1 $aMAX, D. de A. S.
700 1 $aOLIVEIRA, R. S.
700 1 $aSOUZA, F. V. D.
773 $tIn Vitro Cellular & Developmental Biology - Plant, 2020.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
05/10/2023 |
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Data da última atualização: |
23/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
BRITO, L. S. de O.; BATISTA, A.; SANTOS, F. A.; LIMA, R. P. de; AYALA, A. P.; CANUTO, K. M.; SILVEIRA, E. R.; PESSOA, O. D. L. |
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Afiliação: |
LUANA SAN DE OLIVEIRA BRITO, UNIVERSIDADE FEDERAL DO CEARÁ; ALISON BATISTA DA SILVA, UNIVERSIDADE FEDERAL DO CEARÁ; FLÁVIA ALMEIDA SANTOS, UNIVERSIDADE FEDERAL DO CEARÁ; RENAN PEREIRA DE LIMA, UNIVERSIDADE FEDERAL DO CEARÁ; ALEJANDRO PEDRO AYALA, UNIVERSIDADE FEDERAL DO CEARÁ; KIRLEY MARQUES CANUTO, CNPAT; EDILBERTO ROCHA SILVEIRA, UNIVERSIDADE FEDERAL DO CEARÁ; OTILIA DEUSDENIA LOIOLA PESSOA, UNIVERSIDADE FEDERAL DO CEARÁ. |
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Título: |
Anti-inflammatory kaurane diterpenoids of Erythroxylum bezerrae. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Fitoterapia, [Milano], v. 165, art. 105424, Mar. 2023. |
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DOI: |
https://doi.org/10.1016/j.fitote.2022.105424 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Atividade antiinflamatória; Cristalografia de raio-x; Erythroxylum bezerrae; X-ray crystallography. |
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Thesagro: |
Antiinflamatório; Raio X. |
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Thesaurus NAL: |
Anti-inflammatory activity; Crystallography; Erythroxylum; X-ray diffraction. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 00988naa a2200325 a 4500
001 2157119
005 2024-01-23
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.fitote.2022.105424$2DOI
100 1 $aBRITO, L. S. de O.
245 $aAnti-inflammatory kaurane diterpenoids of Erythroxylum bezerrae.$h[electronic resource]
260 $c2023
650 $aAnti-inflammatory activity
650 $aCrystallography
650 $aErythroxylum
650 $aX-ray diffraction
650 $aAntiinflamatório
650 $aRaio X
653 $aAtividade antiinflamatória
653 $aCristalografia de raio-x
653 $aErythroxylum bezerrae
653 $aX-ray crystallography
700 1 $aBATISTA, A.
700 1 $aSANTOS, F. A.
700 1 $aLIMA, R. P. de
700 1 $aAYALA, A. P.
700 1 $aCANUTO, K. M.
700 1 $aSILVEIRA, E. R.
700 1 $aPESSOA, O. D. L.
773 $tFitoterapia, [Milano]$gv. 165, art. 105424, Mar. 2023.
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Embrapa Agroindústria Tropical (CNPAT) |
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