Data/hora: 09/06/2024 03:32:24

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 22/11/2021
Data da última atualização: | 22/11/2021
Autoria: | GUERRA, P. A.; SOUZA, E. H.; MAX, D. A. S.; ROSS, M. L.; VILLALOBOS-OLIVERA, A.; LEDO, C. A. da S.; MARTINEZ-MONTERO, M. E.; SOUZA, F. V. D.
Afiliação: | PATRÍCIA A. GUERRA; EVERTON H. SOUZA; DANIELA A.S. MAX; MÔNICA L. ROSS; ARIEL VILLALOBOS-OLIVERA; CARLOS ALBERTO DA SILVA LEDO, CNPMF; MARCOS E. MARTINEZ-MONTERO; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF.
Título: | Morphoanatomical aspects of the starting material for the improvement of pineapple cryopreservation by the droplet-vitrification technique.
Ano de publicação: | 2021
Fonte/Imprenta: | Anais da Academia Brasileira de Ciências, v.93, n.1, e20190555, [2021?]
ISSN: | 1678-2690
DOI: | 10.1590/0001-3765202120190555
Idioma: | Inglês
Conteúdo: | Cryopreservation of pineapple shoot tips has been established from various protocols, including droplet vitrification. Thus, this work aimed to evaluate the morphoanatomical conditions of the starting material over different times (30, 45 and 60 days) of culture before freezing and its correlation with the survival percentage of the cryopreserved shoot tips. Four accessions, Ananas comosus var. comosus (BGA-009); var. bracteatus (BGA-119); var. parguazensis (BGA-376), var. erectifolius (BGA-750) from the Pineapple Active Germplasm Bank (BGA Pineapple) and two hybrids from the Embrapa Genetic Breeding Program, FIB-ROX1 (var. bracteatus X var. erectifolius) and FIB-ROX2 (var. erectifolius X var. bracteatus), recently introduced in the field from in vitro storage, were used. Histological sections before freezing and the percentages of survival after freezing were obtained taking into account the different times of cultivation of the donor plants. The results showed a significative interaction between genotypes (accessions and hybrids) and the culture period. The accessions BGA-009 and BGA-119 showed the highest survival rates, with 95% and 90% respectively for the 30-day culture time. Different results were obtained for each genotype, showing the need for improvements in the standardization of starting material, which would allow better repeatability of the protocol.
Thesagro: | Abacaxi.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/227998/1/Morphoanatomical-aspects.pdf
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (33426-1) - UPC - AP - DD - Publicação digital -  -  - DIS