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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/04/2000 |
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Data da última atualização: |
04/04/2000 |
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Autoria: |
PEDROSA, A.; SCHWEIZER, D.; GUERRA, M. |
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Afiliação: |
Department of Cytology and Genetics, Institute of Botany, University of Vienna, Rennweg, Vienna, Austria. |
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Título: |
Cytological heterozygosity an d the hybrid origin of sweet orange [citrus sinensis (L.) osbeck]. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Theor Appl Genet, v.100, p.361-367, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus sinensis chromosomes, although small in size, present a remarkable differentiation of bands with the fluorochromes CMA and DAPI. These bands suggest that some heteromorphisms are fixed in this species. To investigate the of these heteromorpisms, ten cultivars of C. sinenesis were analysed with CMA/DAPI staining and, in some of them, the 18S-5.8S rRNA and 5S rRNA genes were located by in situ hybridization. CMA/DAPI staining showed e exactly the same CMA+/DAPI-banding pattern for all cultivars. In situ hybridation revealed three 18S-5.8S-25S rRNA gene sites, two proximally located on two similar chromosomes and one terminally located on a third non-related chromossome. Two 5S rRNA gene sites were observed in this species, with one located proximal to the telomeric 18S-5.8S-25S rRNA site. Both cytological approaches revealed an invariable, heterozigotic karyotype among sweet orange cultivars. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01326naa a2200145 a 4500
001 1648740
005 2000-04-04
008 2000 bl --- 0-- u #d
100 1 $aPEDROSA, A.
245 $aCytological heterozygosity an d the hybrid origin of sweet orange [citrus sinensis (L.) osbeck].
260 $c2000
520 $aCitrus sinensis chromosomes, although small in size, present a remarkable differentiation of bands with the fluorochromes CMA and DAPI. These bands suggest that some heteromorphisms are fixed in this species. To investigate the of these heteromorpisms, ten cultivars of C. sinenesis were analysed with CMA/DAPI staining and, in some of them, the 18S-5.8S rRNA and 5S rRNA genes were located by in situ hybridization. CMA/DAPI staining showed e exactly the same CMA+/DAPI-banding pattern for all cultivars. In situ hybridation revealed three 18S-5.8S-25S rRNA gene sites, two proximally located on two similar chromosomes and one terminally located on a third non-related chromossome. Two 5S rRNA gene sites were observed in this species, with one located proximal to the telomeric 18S-5.8S-25S rRNA site. Both cytological approaches revealed an invariable, heterozigotic karyotype among sweet orange cultivars.
700 1 $aSCHWEIZER, D.
700 1 $aGUERRA, M.
773 $tTheor Appl Genet$gv.100, p.361-367, 2000.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 19. |  | NODARI, R. O.; REIS, A.; GUERRA, M. P.; REIS, M. S. dos; FLORIANO, E. P. Analise preliminar do inventario do palmiteiro em floresta ombrofila densa montana. In: ENCONTRO NACIONAL DE PESQUISADORES DE PALMITO, 1., 1987, Curitiba. Anais. Curitiba: EMBRAPA-CNPF, 1988. p.159-164. (EMBRAPA-CNPF. Documentos, 19).| Biblioteca(s): Embrapa Florestas. |
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