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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
24/09/2018 |
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Data da última atualização: |
24/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA FILHO, J. X. de; MORES, M. A. Z.; REBELATTO, R.; KICH, J. D.; CANTAO, M. E.; KLEIN, C. S.; GUEDES, R. M. C.; COLDEBELLA, A.; BARCELLOS, D. E. S. N. de; MORES, N. |
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Afiliação: |
JOÃO XAVIER DE OLIVEIRA FILHO; MARCOS ANTONIO ZANELLA MORES, CNPSA; RAQUEL REBELATTO, CNPSA; JALUSA DEON KICH, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; CATIA SILENE KLEIN, CNPSA; ROBERTO MAURÍCIO CARVALHO GUEDES; ARLEI COLDEBELLA, CNPSA; DAVID EMÍLIO SANTOS NEVES de BARCELLOS; NELSON MORES, CNPSA. |
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Título: |
Pathogenic variability among Pasteurella multocida type A isolates from Brazilian pig farms. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
BMC Veterinary Research, v. 14, n. 244, 2018. |
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DOI: |
10.1186/s12917-018-1565-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Background: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). Results: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic strain, and an insertion of 37 kb of phage DNA was observed in the nonpathogenic strains. MLST clustered the HP isolates in one group and the low and nonpathogenic isolates in another. Only the nonpathogenic isolates matched sequence type 10; the other isolates did not match any type available in the MLST database. Conclusions: The hypothesis that some PmA strains are primary pathogens and cause disease in pigs without any co-factor was confirmed. The pfhA region, comprising the genes tpsB1, tpsB2, pfhA1 and pfhA2, is related to the pathogenicity of PmA. The HP strains can cause necrotic bronchopneumonia, fibrinous pleuritis and pericarditis in pigs and can be identified by PCR amplification of the gene pfhA2. MenosAbstract: Background: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). Results: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic ... Mostrar Tudo |
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Palavras-Chave: |
Broncopneumonia; PCR; PfhA; Poliserositis; Polyserositis. |
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Thesagro: |
Doença Respiratória; Suíno. |
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Thesaurus Nal: |
Bronchopneumonia; Multilocus sequence typing; Pathogenicity; Polymerase chain reaction; Respiratory tract diseases; Swine; Virulent strains. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03381naa a2200409 a 4500
001 2096196
005 2018-09-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1186/s12917-018-1565-2$2DOI
100 1 $aOLIVEIRA FILHO, J. X. de
245 $aPathogenic variability among Pasteurella multocida type A isolates from Brazilian pig farms.$h[electronic resource]
260 $c2018
520 $aAbstract: Background: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). Results: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic strain, and an insertion of 37 kb of phage DNA was observed in the nonpathogenic strains. MLST clustered the HP isolates in one group and the low and nonpathogenic isolates in another. Only the nonpathogenic isolates matched sequence type 10; the other isolates did not match any type available in the MLST database. Conclusions: The hypothesis that some PmA strains are primary pathogens and cause disease in pigs without any co-factor was confirmed. The pfhA region, comprising the genes tpsB1, tpsB2, pfhA1 and pfhA2, is related to the pathogenicity of PmA. The HP strains can cause necrotic bronchopneumonia, fibrinous pleuritis and pericarditis in pigs and can be identified by PCR amplification of the gene pfhA2.
650 $aBronchopneumonia
650 $aMultilocus sequence typing
650 $aPathogenicity
650 $aPolymerase chain reaction
650 $aRespiratory tract diseases
650 $aSwine
650 $aVirulent strains
650 $aDoença Respiratória
650 $aSuíno
653 $aBroncopneumonia
653 $aPCR
653 $aPfhA
653 $aPoliserositis
653 $aPolyserositis
700 1 $aMORES, M. A. Z.
700 1 $aREBELATTO, R.
700 1 $aKICH, J. D.
700 1 $aCANTAO, M. E.
700 1 $aKLEIN, C. S.
700 1 $aGUEDES, R. M. C.
700 1 $aCOLDEBELLA, A.
700 1 $aBARCELLOS, D. E. S. N. de
700 1 $aMORES, N.
773 $tBMC Veterinary Research$gv. 14, n. 244, 2018.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 16 | |
| 1. |  | PALADINO, E. S.; GABARDO, M. de P.; LUNARDI, P. N.; MORES, N.; GUEDES, R. M. C. Anatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n, 10, p. 1091-1100, outubro 2017. Título em português: Aspectos pneumônicos anatomo-patológicos associados a Pasteurella multocida de alta patogenicidade em suínos de terminação.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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| 4. | | DALLANORA, D.; PIEROZAN, R. L.; KICH, J. D.; MACHADO, G. S.; COLDEBELLA, A. MORÉS, N.; GUEDES, R. M. C. Comparative efficacy of tiamulin (denagard®) associated with chlortetracycline (CTC), florfenicol and tilmicosin on Mycoplasma hyopneumoniae -infected pigs experimentally inoculated with Actinobacillus pleuropneumoniae and Pasteurella multocida type A. In: INTERNATIONAL PIG VETERINARY SOCIETY, 20., 2008, Durban, South Africa. Proceedings. Durban: IPVS, 2008. p. 498| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 5. | | DALLANORA, D.; PIEROZAN, R. L.; KICH, J. D.; MACHADO, G. S.; COLDEBELLA, A. MORÉS, N.; GUEDES, R. M. C. Comparative speed of action of different drugs used for the control of swine respiratory disease. In: INTERNATIONAL PIG VETERINARY SOCIETY, 20., 2008, Durban, South Africa. Proceedings. Durban: IPVS, 2008. p. 378 Projeto/Plano de Ação: 10.07.11013-0.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 6. | | DIAS, A. S.; COSTA, E. A.; RAJÃO, D. de S.; GUEDES, R. M. C.; ZANELLA, J. R. C.; LOBATO, Z. I. P. Distribution of antibodies against influenza virus in pigs from farrow-to-finish farms in Minas Gerais state, Brazil. Influenza and Other Respiratory Viruses, v. 3, p. 161-167, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | SATO, J. P. H.; PAGNO, K. A.; MARQUES, B. M. F. P. P.; GAVA, D.; SCHAEFER, R.; PISSETTI, C.; GUEDES, R. M. C. A high mortality of H1N1pdm vaccinated sows related with H1N2 subtype. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 26., 2022, Rio de Janeiro. Proceedings... Rio de Janeiro, RJ: IPVS: Abraves, 2022. p. 368.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | PALADINO, E. S.; GABARDO, M. P.; LUNARDI, P. N.; OLIVEIRA FILHO, J. X.; MORES, M. A. Z.; MORES, N.; GUEDES, R. M. C. Profile of porcine respiratory disease complex associated with Pasteurella multocida in Minas Gerais state, Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 22., 2012, Jeju. Proceedings... Jeju: IPVS, 2012. p. 763. Projeto/Plano de Ação: 02.10.61600-01.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 9. | | MENEGUZZI, M.; PISSETTI, C.; REBELATTO, R.; TRACHSEL, J.; KUCHIISHI, S. S.; REIS, A. T.; GUEDES, R. M. C.; LEÃO, J. A.; REICHEN, C.; KICH, J. D. Re-emergence of salmonellosis in hog farms: outbreak and bacteriological characterization. Microorganisms, v. 9, n. 947. 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 10. | | MENEGUZZI, M.; PISSETTI, C.; REBELATTO, R.; KUCHIISHI, S. S.; COSTA, A. T. R.; GUEDES, R. M. C.; LEÃO, J. A.; KICH, J. D. Resistência a colistina em isolados de samonella de casos clínicos de suínos no Brasil. In: CONGRESSO DA ABRAVES, 18., 2017, Goiânia. Suinocultura brasileira na era da tecnologia e da sustentabilidade: anais. Concórdia: Embrapa Suínos e Aves, 2017. v. 1. Palestras. p. 27-28.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 11. | | PEREIRA, C. E. R.; VANNUCCI, F. A.; GABARDO, M. de P.; SANTOS, L. F. dos; MORES, N.; GUEDES, R. M. C. Mycoplasma hyorhinis infection in early cases of mycoplasmal pneumonia in swine and evaluation of diagnostic assays. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n, 10, p. 1057-1063, outubro 2017. Título em português: Infecção por Mycoplasma hyorhinis em casos precoces de pneumonia micoplásmica em suínos e comparação entre técnicas diagnósticas.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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| 12. | | MENEGUZZI, M.; KICH, J. D.; REBELATTO, R.; PISSETTI, C.; KUCHIISHI, S. S.; REIS, A. T.; GUEDES, R. M. C.; LEÃO, J. A.; REICHEN, C. Salmonella clinical isolates from Brazilian pig herds: genetic relationship and antibiotic resistance profiling. In: INTERNATIONAL SYMPOSIUM ON THE EPIDEMIOLOGY AND CONTROL OF BIOLOGICAL, CHEMICAL AND PHYSICAL HAZARDS IN PIG AND PORK, 12., 2017, Foz do Iguaçu. Proceedings book... Concórdia: Embrapa Suínos e Aves, 2017. p. 170-174. SafePork 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 13. | | OLIVEIRA FILHO, J. X. de; MORES, M. A. Z.; REBELATTO, R.; KICH, J. D.; CANTAO, M. E.; KLEIN, C. S.; GUEDES, R. M. C.; COLDEBELLA, A.; BARCELLOS, D. E. S. N. de; MORES, N. Pathogenic variability among Pasteurella multocida type A isolates from Brazilian pig farms. BMC Veterinary Research, v. 14, n. 244, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 14. | | DANIEL, A. G. S.; SATO, J. P. H.; GABARDO, M. P.; RESENDE, T. P.; BARCELLOS, D. E. S. N. de; PEREIRA, C. E. R.; VANNUCCI, F. A.; GUEDES, R. M. C. Minimum inhibitory concentration of Brazilian Brachyspira hyodysenteriae strains. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 4, p. 331-338, abril 2017. Título em português: Concentração inibitória mínima de cepas Brachyspira hyodysenteriae brasileiras.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 15. | | RAJÃO, D. S.; ALVES, F.; DEL PUERTO, H. L.; BRAZ, G. F.; OLIVEIRA, F. G.; ZANELLA, J. R. C.; SCHAEFER, R.; REIS, J. K. P. dos; GUEDES, R. M. C.; LOBATO, Z. I. P.; LEITE, R. C. Serological evidence of swine influenza in Brazil. Influenza and Other Respiratory Viruses, v. 7, n. 2, p. 109-112, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 16. | | MORES, N.; MORES, M. A. Z.; OLIVEIRA FILHO, J. X.; KLEIN, C. S.; CARON, L.; COLDEBELLA, A.; REBELATTO, R.; SLUSZZ, T.; GUEDES, R. M. C.; ALBERTON, G. C.; DUTRA, V.; MORENO, A. H.; SOBESTIANSKY, J; MATOS, M. P. C.; BARCELLOS, D. S. N. Study of the pathogenesis and development of diagnostic methods and control of pneumonic pasteurellosis in pigs. In: SIMPÓSIO EMBRAPA LabEx EUA DE SANIDADE ANIMAL, 2., 2012, Brasília. Anais... Brasília: Embrapa Estudos e Capacitação, 2012. p. 68-69. Projeto/Plano de Ação: 02.10.06.016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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