| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
28/12/2023 |
|
Data da última atualização: |
22/01/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ROSA, P. M. da S.; GUEDES, P. H. E.; GARCIA, J. M.; OLIVEIRA, C. S. |
|
Afiliação: |
PAOLA MARIA DA SILVA ROSA, UNIVERSIDADE ESTADUAL PAULISTA; PEDRO HENRIQUE EVAGELISTA GUEDES; JOAQUIM MANSANO GARCIA, UNIVERSIDADE ESTADUAL PAULISTA; CLARA SLADE OLIVEIRA, CNPGL. |
|
Título: |
Cytoplasmic granules in bovine oocytes do not affect embryonic or fetal development. |
|
Ano de publicação: |
2023 |
|
Fonte/Imprenta: |
Zygote, v. 32, n. 1, p. 28-37, 2023. |
|
DOI: |
https://doi.org/10.1017/S0967199423000576 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Oocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence. MenosOocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytopla... Mostrar Tudo |
|
Palavras-Chave: |
Classificação morfológica; IVF; Oócito; Ooplasma; Ovócito. |
|
Thesagro: |
Bovino; Citoplasma; Embrião Animal; Granulo. |
|
Thesaurus Nal: |
Cytoplasm; Granules. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02681naa a2200301 a 4500
001 2160283
005 2024-01-22
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199423000576$2DOI
100 1 $aROSA, P. M. da S.
245 $aCytoplasmic granules in bovine oocytes do not affect embryonic or fetal development.$h[electronic resource]
260 $c2023
520 $aOocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence.
650 $aCytoplasm
650 $aGranules
650 $aBovino
650 $aCitoplasma
650 $aEmbrião Animal
650 $aGranulo
653 $aClassificação morfológica
653 $aIVF
653 $aOócito
653 $aOoplasma
653 $aOvócito
700 1 $aGUEDES, P. H. E.
700 1 $aGARCIA, J. M.
700 1 $aOLIVEIRA, C. S.
773 $tZygote$gv. 32, n. 1, p. 28-37, 2023.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 8 | |
| 2. |  | GUEDES, P. H. E.; DIAS, H. R. S.; FREITAS, C. de; CAMARGO, A. J. dos R.; PINNA, A. E.; NOGUEIRA, L. A. G. N.; OLIVEIRA, C. S. Post implantation measurements of female PIVE embryos can show risk of pregnancy loss in cows. In: Annual Meeting of the Brazilian Embryo Technology Society, 33rd. Resumo em Anais. Ilha de Comandatuba, Bahia. SBTE, 2019. 657.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
|    |
| 3. | | OLIVEIRA, C. S.; MARQUES, S. C. S.; GUEDES, P. H. E.; FEUCHARD, V. L.; CAMARGO, A. J. R.; FREITAS, C. de; CAMARGO, L. S. de A. Thermal-treatment protocol to induce thermotolerance in bovine embryos. Reproduction, Fertility and Development, v. 33, n. 7, p. 497-501, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 4. | | GUEDES, P. H. E.; OLIVEIRA, C. S.; DIAS, H. R. S.; FREITAS, C. de; CAMARGO, A. J. R.; SARAIVA, N. Z.; CAMARGO, L. S. de A. Ultrasound characteristics and pregnancy loss in bovine IVF-derived pregnancies. Livestock Science, v. 257, 104847, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 5. | | GUEDES, P. H. E.; PINNA, A. E.; MORAIS, R. de C. L.; SOUZA, G. N. de; JACOB, J. C. F.; CARVALHO, C. F. P. de M.; NOGUEIRA, L. A. G.; OLIVEIRA, C. S. Doppler evaluation of recipient cows treated with progesterone and estradiol. Animal Production Science, v. 62, n. 9, p. 836-843, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 6. | | OLIVEIRA, C. S.; GUEDES, P. H. E.; MARQUES, S. C. S.; SILVA, J. V. G.; CAMARGO, A. J. R.; FREITAS, C. de; SERAPIÃO, R. V.; Saraiva, N. Z.; CAMARGO, L. S. de A. Induction of survival mechanisms to thermal stress in bovine embryos. Animal Reproduction, v. 15, n. 3, p. 481, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 7. | | OLIVEIRA, C. S.; ROSA, P. M. da S.; ALVES, B. R. C.; MONTEIRO, C. A. S.; LEAL, G. R.; GUEDES, P. H. E.; CAMARGO, A. J. dos R.; SARAIVA, N. Z. Luteinizing hormone secretion, ovulatory capacity, and oocyte quality in peripubertal Gir heifers. Zygote, v. 31, n. 4, p. 342-349, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 8. | | GUEDES, P. H. E.; ROSA, P. M. S.; DIAS, H. R. S.; SERAPIÃO, R. V.; CAMARGO, A. J. R.; FREITAS, C. de; NOGUEIRA, L. A. G.; PINNA. A. E.; SILVA, J. V. G.; MARQUES, S. C. S.; OLIVEIRA, C. S. Morphometry after implantation of bovine embryos submitted to thermal stress during IVP. Animal Reproduction, v. 15, n. 3, p. 479, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| Registros recuperados : 8 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
