|
|
Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Uva e Vinho. |
Data corrente: |
14/03/2024 |
Data da última atualização: |
14/03/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FAJARDO, T. V. M.; GRYNBERG, P.; TOGAWA, R. C.; SILVA, J. M. F.; SILVA, F. N. da; NICKEL, O. |
Afiliação: |
THOR VINICIUS MARTINS FAJARDO, CNPUV; PRISCILA GRYNBERG, Cenargen; ROBERTO COITI TOGAWA, Cenargen; JOÃO MARCOS FAGUNDES SILVA, INSTITUTE FOR INTEGRATIVE SYSTEMS BIOLOGY; FABIO NASCIMENTO DA SILVA, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; OSMAR NICKEL, CNPUV. |
Título: |
Analyzes of mealybug (Pseudococcus longispinus) virome reveal grapevine viruses diversity. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Tropical Plant Pathology, [online], March 2024. |
DOI: |
https://doi.org/10.1007/s40858-024-00647-3 |
Idioma: |
Inglês |
Conteúdo: |
The long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps. longispinus virome in Brazil focusing on grapevine viruses. MenosThe long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps... Mostrar Tudo |
Palavras-Chave: |
High throughput sequencing; Vector. |
Thesaurus Nal: |
Ampelovirus; Closterovirus; Vitivirus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1162846/1/s40858-024-00647-3.pdf
|
Marc: |
LEADER 02312naa a2200253 a 4500 001 2162846 005 2024-03-14 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s40858-024-00647-3$2DOI 100 1 $aFAJARDO, T. V. M. 245 $aAnalyzes of mealybug (Pseudococcus longispinus) virome reveal grapevine viruses diversity.$h[electronic resource] 260 $c2024 520 $aThe long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps. longispinus virome in Brazil focusing on grapevine viruses. 650 $aAmpelovirus 650 $aClosterovirus 650 $aVitivirus 653 $aHigh throughput sequencing 653 $aVector 700 1 $aGRYNBERG, P. 700 1 $aTOGAWA, R. C. 700 1 $aSILVA, J. M. F. 700 1 $aSILVA, F. N. da 700 1 $aNICKEL, O. 773 $tTropical Plant Pathology, [online], March 2024.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Acre. Para informações adicionais entre em contato com cpafac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Acre. |
Data corrente: |
16/01/2008 |
Data da última atualização: |
10/11/2023 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
COSTA, F. H. da S.; PASQUAL, M.; SANTOS, A. M. dos; CASTRO, E. M. de; PEREIRA, J. E. S.; PIO, L. A.; COSTA, L. C. do. |
Afiliação: |
FREDERICO HENRIQUE DA SILVA COSTA, UFLA; MOACIR PASQUAL, UFLA; ADRIENE MATOS DOS SANTOS, UFLA; EVARISTO MAURO DE CASTRO, UFLA; JONNY EVERSON SCHERWINSKI PEREIRA, CPAF-AC; LEILA APARECIDA PIO, UFLA; LARISSA CORREA DO BOMFIM COSTA, UFLA. |
Título: |
Relação entre o conteúdo relativo de água, densidade estomática e formação de cera epicuticular em folhas de plantas micropropagadas de bananeira. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 16.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 3.; SIMPÓSIO DE PLANTAS ORNAMENTAIS NATIVAS, 1., 2007, Goiânia. Biodiversidade e competitividade: buscando novas opções: anais. Campinas: Sociedade Brasileira de Floricultura e Plantas Ornamentais, 2007. |
Páginas: |
p. 1145-1148. |
Idioma: |
Português |
Palavras-Chave: |
Musa spp. |
Thesagro: |
Água; Banana. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00985nam a2200217 a 4500 001 1505915 005 2023-11-10 008 2007 bl uuuu u00u1 u #d 100 1 $aCOSTA, F. H. da S. 245 $aRelação entre o conteúdo relativo de água, densidade estomática e formação de cera epicuticular em folhas de plantas micropropagadas de bananeira.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 16.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 3.; SIMPÓSIO DE PLANTAS ORNAMENTAIS NATIVAS, 1., 2007, Goiânia. Biodiversidade e competitividade: buscando novas opções: anais. Campinas: Sociedade Brasileira de Floricultura e Plantas Ornamentais$c2007 300 $ap. 1145-1148. 650 $aÁgua 650 $aBanana 653 $aMusa spp 700 1 $aPASQUAL, M. 700 1 $aSANTOS, A. M. dos 700 1 $aCASTRO, E. M. de 700 1 $aPEREIRA, J. E. S. 700 1 $aPIO, L. A. 700 1 $aCOSTA, L. C. do
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Acre (CPAF-AC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|