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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
01/09/2015 |
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Data da última atualização: |
29/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. B.; LOBO JUNIOR, M.; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. |
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Afiliação: |
MARÍLIA BARROS OLIVEIRA, UFG; MURILLO LOBO JUNIOR, CNPAF; MARIA FATIMA GROSSI DE SA, CENARGEN; SILVANA PETROFEZA, UFG. |
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Título: |
Exogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Plant Physiology, v. 182, p. 13-22, June 2015. |
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DOI: |
10.1016/j.jplph.2015.04.006 |
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Idioma: |
Inglês |
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Conteúdo: |
Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris - S. sclerotiorum pathosystem. MenosSclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalan... Mostrar Tudo |
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Palavras-Chave: |
Genes relacionadas com patogénese. |
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Thesagro: |
Doença de planta; Feijão; Fungo; Mofo Branco; Phaseolus vulgaris; Sclerotinia sclerotiorum. |
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Thesaurus Nal: |
Dry beans; Methyl jasmonate. |
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Categoria do assunto: |
--
H Saúde e Patologia |
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Marc: |
LEADER 02883naa a2200277 a 4500
001 2025021
005 2016-03-29
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jplph.2015.04.006$2DOI
100 1 $aOLIVEIRA, M. B.
245 $aExogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants.$h[electronic resource]
260 $c2015
520 $aSclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris - S. sclerotiorum pathosystem.
650 $aDry beans
650 $aMethyl jasmonate
650 $aDoença de planta
650 $aFeijão
650 $aFungo
650 $aMofo Branco
650 $aPhaseolus vulgaris
650 $aSclerotinia sclerotiorum
653 $aGenes relacionadas com patogénese
700 1 $aLOBO JUNIOR, M.
700 1 $aGROSSI-DE-SÁ, M. F.
700 1 $aPETROFEZA, S.
773 $tJournal of Plant Physiology$gv. 182, p. 13-22, June 2015.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 368 | |
| 22. |  | CORDEIRO, M. C. R.; SILVA, M. S.; MARTINS, N. F.; GROSSI-DE-SÁ, M. F. Expressão de fatores de transcrição tipo WRKY no banco Genoma Funcional de café (Coffea sp.). In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007. Biotecnologia aplicada à cadeia agroindustrial do café.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 23. | | ARANTES, I. C.; MULINARI, F.; QUEZADO, M.; GROSSI-DE-SÁ, M. F. Expressão heteróloga do inibidor de proteinase BTCI em Escherichia coli. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 81.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 24. | | BITTAR, P. D.; VIANA, A. A. B.; GROSSI-de-SÁ, M. F.; FREITAS, S. M. Expressão heteróloga de um inibidor de serinoprotease (BTCI) de Vigna unguiculata em plantas de Nicotiana tabacum. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. A era da genômica: da bioestatística à bioinformática: anais. Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2005. p. 625.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 25. | | DEL-SARTO, R. P.; SILVA, F. B.; MONTEIRO, A. C. S.; GROSSI-de-SÁ, M. F. Expressão da região pro de proteinase cisteínica de Acanthoscelides obtectus. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 47.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 28. | | ARBOLEDA, V. J. W.; JIMÉNEZ, A. V.; SILVA, M. S.; GROSSI DE SÁ, M. F. Caracterização parcial de enzimas proteolíticas intestinais de larvas de broca gigante da cana, Castnia licus (Drury). In: WORKSHOP INTERAÇÃO MOLECULAR PLANTA-PRAGAS, 2., 2007, Brasília, DF. II Workshop Interação Molecular Planta-Praga. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 43-48. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 229).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 34. | | BEVITORI, R.; POPIELARSKA-KONIECZNA, M.; SANTOS, E. M. dos; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation. Protoplasma, New York, v. 251, n. 3, p. 545-554, May 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 35. | | MARCELLINO, L. H.; NESHICH, G.; GROSSI DE SA, M. F.; KREBBERS, E.; GANDER, E. S. Modified 2S albumins with improved tryptophan content are correctly expressed in transgenic tobacco plants. FEBS Letters, Amsterdam, v.385, n.3, p.154-158, 1996. p.154-158| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 38. | | LIMA, T. B.; GROSSI DE SÁ, M. F.; FRANCO, O. L.; LEONARDECZ, E. Phytochemistry characterization of the effect of Cagaita (Eugenia dysenterica) in laxative and antidiarreic compound. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts. [S.l.]: SBBq, 2007. Não paginado.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 40. | | FRANCO, O. L.; RIGDEN, D. J.; MELO, F. R.; GROSSI-DE-SÁ, M. F. Plant alpha-amylase inhibitors and their interaction with insect alfa-amylases: structure, function and potential for crop protection. European Journal of Biochemistry, New York, NY, v. 269, n. 2, p. 397-412, 2002.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 368 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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