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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
14/01/2011 |
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Data da última atualização: |
17/04/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, OSMUNDO B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. |
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Afiliação: |
ÉRICO A. R. VASCONCELOS, UNB; CELSO G. SANTANA; CLAUDIA VIEIRA GODOY, CNPSO; MARILIA SANTOS SILVA, CPAC; LEONORA R. S. MOREIRA, UNB; OSMUNDO B. OLIVEIRA-NETO; DANIEL PRICE, Durham University; ELAINE FITCHES, Durham University; EDIVALDO X. F. FILHO, UNB; ANGELA MEHTA DOS REIS, CENARGEN; JOHN A. GATEHOUSE, Durham University; MARIA FATIMA GROSSI DE SA, CENARGEN. |
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Título: |
A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control. |
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Palavras-Chave: |
Asian rust. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31109/1/cbb.errata.cvg.pdf
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Marc: |
LEADER 02303nam a2200253 a 4500
001 1873147
005 2018-04-17
008 2010 bl uuuu u00u1 u #d
100 1 $aVASCONCELOS, E. A. R.
245 $aA new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
260 $aIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec$c2010
520 $aAsian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control.
653 $aAsian rust
700 1 $aSANTANA, C. G.
700 1 $aGODOY, C. V.
700 1 $aSILVA, M. S.
700 1 $aMOREIRA, L. R. S.
700 1 $aOLIVEIRA-NETO, OSMUNDO B.
700 1 $aPRICE, D.
700 1 $aFITCHES, E.
700 1 $aFILHO, E. X. F.
700 1 $aMEHTA, A.
700 1 $aGATEHOUSE, J. A.
700 1 $aGROSSI-DE-SA, M. F.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 40 | |
| 22. |  | OLIVEIRA, E. T.; POPINIGIS, F.; FONSECA, J. R.; ANDRIGUETTO, J. R.; MIRANDA, L. C. Pós controle de qualidade de sementes básicas de feijão (Phaseolus vulgaris L.). In: REUNIÃO NACIONAL DE PESQUISA DE FEIJÃO, 2., 1987, Goiânia. Resumos. Brasilia, DF: EMBRAPA-CNPAF, 1987. Resumo 180. (EMBRAPA-CNPAF. Documentos, 20).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 32. | | FRAZAO, D. A. C.; FIGUEIREDO, F. J. C.; CARVALHO, J. E. U. de; POPINIGIS, F.; OLIVEIRA, R. P. de. Emergência e vigor de sementes de guaraná submetidas a pré-tratamentos térmicos. In: CONGRESSO BRASILEIRO DE SEMENTES, 2., 1981, Recife. Resumo dos trabalhos técnicos... Brasília, DF: ABRATES, 1981. p. 56.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 36. | | FRAZAO, D. A. C.; FIGUEIREDO, F. J. C.; CORREA, M. P. F.; OLIVEIRA, R. P. de; POPINIGIS, F. Tamanho da semente de guaraná e sua influencia na emergência e no vigor. In: CONGRESSO BRASILEIRO DE SEMENTES, 2., 1981, Recife. Resumo dos trabalhos técnicos... Brasília, DF: ABRATES, 1981. p. 91.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 40. | | FRAZAO, D. A. C.; FIGUEIREDO, F. J. C.; POPINIGIS, F.; CORREA, M. P. F.; GUEDES, A. L. C.; KATO, O. R. Ação de substâncias químicas na germinação de sementes de guaraná. In: CONGRESSO BRASILEIRO DE SEMENTES, 1., 1979, Curitiba. Resumos dos trabalhos técnicos... Curitiba: ABRATES, 1979. p. 67.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 40 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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