Data/hora: 03/06/2024 10:16:52

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido; Embrapa Tabuleiros Costeiros.
Data corrente: | 04/09/2020
Data da última atualização: | 27/12/2020
Autoria: | NORIEGA, D. D.; ARRAES, F. B. M.; ANTONINO, J. D.; MACEDO, L. L. P.; FONSECA, F. C. A.; TOGAWA, R. C.; GRYNBERG, P.; SILVA, M. C. M.; NEGRISOLI JUNIOR, A. S.; MORGANTE, C. V.; GROSSI-DE-SA, M. F.
Afiliação: | DANIEL D. NORIEGA, UNB; FABRICIO B. M. ARRAES, UFRGS; JOSÉ DIJAIR ANTONINO, UFRPE; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; FERNANDO C. A. FONSECA, UNB; ROBERTO COITI TOGAWA, Cenargen; PRISCILA GRYNBERG, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ALDOMARIO SANTO NEGRISOLI JUNIOR, CPATC; CAROLINA VIANNA MORGANTE, CPATSA; MARIA FATIMA GROSSI DE SA, Cenargen.
Título: | Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source.
Ano de publicação: | 2020
Fonte/Imprenta: | PLoS ONE, v. 15, n. 8, e0235575, 2020.
DOI: | https://doi.org/10.1371/journal.pone.0235575
Idioma: | Inglês
Conteúdo: | The sugarcane borer (Diatraea saccharalis, Fabricius, 1794) is a devastating pest that causes millions of dollars of losses each year to sugarcane producers by reducing sugar and ethanol yields. The control of this pest is difficult due to its endophytic behavior and rapid development. Pest management through biotechnological approaches has emerged in recent years as an alternative to currently applied methods. Genetic information about the target pests is often required to perform biotechnology-based management. The genomic and transcriptomic data for D. saccharalis are very limited. Herein, we report a tissue-specific transcriptome of D. saccharalis larvae and a differential expression analysis highlighting the physiological characteristics of this pest in response to two different diets: sugarcane and an artificial diet. Sequencing was performed on the Illumina HiSeq 2000 platform, and a de novo assembly was generated. A total of 27,626 protein-coding unigenes were identified, among which 1,934 sequences were differentially expressed between treatments. Processes such as defence,  igestion, detoxification, signaling, and transport were highly represented among the differentially expressed genes (DEGs). Furthermore, seven aminopeptidase genes were identified as candidates to encode receptors of Cry proteins, which are toxins of Bacillus thuringiensis used to control lepidopteran pests. Since plantinsect interactions have produced a considerable number of adaptive responses in hosts and herbivorous insects, the success of phytophagous insects relies on their ability to overcome challenges such as the response to plant defences and the intake of nutrients. In this study, we identified metabolic pathways and specific genes involved in these processes. Thus, our data strongly contribute to the knowledge advancement of insect transcripts, which can be a source of target genes for pest management
Palavras-Chave: | Controle biotecnológico; Controle de praga; D saccharalis; Genetic Aspects; Manejo da praga; Physiological Aspects.
Thesagro: | Biotecnologia; Cana de Açúcar; Diatraea Saccharalis; Dieta; Doença; Praga.
Thesaurus Nal: | Biotechnology; Genomics; Pest control; Pest management; Sugarcane; Transcription factors.

Categoria do assunto:

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O Insetos e Entomologia

URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/215768/1/journal.pone.0235575.pdf
Registro original: | Embrapa Tabuleiros Costeiros (CPATC)

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