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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
23/03/2009 |
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Data da última atualização: |
08/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GAMA, M. A. S.; GARNSWORTHY, P. C.; GRIINARI, J. M.; LEME, P. R.; RODRIGUES, P. H. M.; SOUZA, L. W. O.; LANNA, D. P. D. |
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Afiliação: |
Marco Antonio Sundfeld da Gama, Embrapa Gado de Leite; P. C. Garnsworthy University of Nottingham School of Biosciences Loughborough UK; J. M. Griinari University of Helsinki Finland; P. R. Leme, USP; P. H. M. Rodrigues, USP; L. W. O. Souza, USP; Dante Pazzanese Duarte Lanna, ESALQ/USP. |
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Título: |
Diet - inducet milk fat depression: Assoication with changes in milk fatty acid composition and fluidity of milk fat. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Livestock Science, v. 115, n. 2/3, p. 319-331, 2008. |
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DOI: |
https://doi.org/10.1016/j.livsci.2007.08.006 |
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Idioma: |
Inglês |
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Conteúdo: |
This experiment was designed to examine changes in milk fatty acids during fish oil-induced milk fat depression (MFD) and to test the theory that these changes are related to milk fat fluidity. The experiment was divided into three periods: 1) Baseline: all cows (n = 12) received a high fiber diet without fish oil (FO) for 12 days; 2) Treatment: 4 cows/group received the following treatments for 21 days: a) Low fiber diet without FO (LF), b) High fiber diet + FO (HF + FO) and c) Low fiber diet + FO (LF + FO); 3) Post-treatment: cows returned to the baseline diet and were monitored for 12 days. FO was included at 1.6% DM and HF and LF diets had 40 and 26% NDF, respectively. Milk fat content and yield were unchanged by the LF diet, but were reduced by FO diets at both dietary fiber levels and recovered in the post-treatment period. FO diets caused a pronounced reduction in stearic and oleic acid concentrations in milk fat and an equally pronounced increase in trans-18:1 fatty acid concentrations. Milk fat mean melting point (MMP) was correlated with MFD (r = 0.73) and with milk oleic acid concentration (r = − 0.92). The ratio of oleic:stearic in milk fat increased gradually and consistently in response to FO. Trans-C18:1 isomers with double bounds at carbon ≤ 10 increased with greater MFD and those with double bonds at carbon ≥ 11 decreased with greater MFD. Trans-9 cis-11 CLA explained more than 80% of MFD and was strongly correlated with trans-10 C18:1. Maintenance of MMP below 39–40 °C suggests that the mammary gland was able to secrete only milk fat with adequate fluidity and that MFD could be an adaptation mechanism to prevent secretion of milk with higher MMP. MenosThis experiment was designed to examine changes in milk fatty acids during fish oil-induced milk fat depression (MFD) and to test the theory that these changes are related to milk fat fluidity. The experiment was divided into three periods: 1) Baseline: all cows (n = 12) received a high fiber diet without fish oil (FO) for 12 days; 2) Treatment: 4 cows/group received the following treatments for 21 days: a) Low fiber diet without FO (LF), b) High fiber diet + FO (HF + FO) and c) Low fiber diet + FO (LF + FO); 3) Post-treatment: cows returned to the baseline diet and were monitored for 12 days. FO was included at 1.6% DM and HF and LF diets had 40 and 26% NDF, respectively. Milk fat content and yield were unchanged by the LF diet, but were reduced by FO diets at both dietary fiber levels and recovered in the post-treatment period. FO diets caused a pronounced reduction in stearic and oleic acid concentrations in milk fat and an equally pronounced increase in trans-18:1 fatty acid concentrations. Milk fat mean melting point (MMP) was correlated with MFD (r = 0.73) and with milk oleic acid concentration (r = − 0.92). The ratio of oleic:stearic in milk fat increased gradually and consistently in response to FO. Trans-C18:1 isomers with double bounds at carbon ≤ 10 increased with greater MFD and those with double bonds at carbon ≥ 11 decreased with greater MFD. Trans-9 cis-11 CLA explained more than 80% of MFD and was strongly correlated with trans-10 C18:1. Maintenance of MMP be... Mostrar Tudo |
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Palavras-Chave: |
Conjugated linoleic acids; Fish oil; Milk fat depression; Milk fat fluidity; Trans-fatty acids. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/596411/1/Diet-inducet-milk-fat-depression.pdf
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Marc: |
LEADER 02550naa a2200265 a 4500
001 1596411
005 2024-02-08
008 2008 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.livsci.2007.08.006$2DOI
100 1 $aGAMA, M. A. S.
245 $aDiet - inducet milk fat depression$bAssoication with changes in milk fatty acid composition and fluidity of milk fat.$h[electronic resource]
260 $c2008
520 $aThis experiment was designed to examine changes in milk fatty acids during fish oil-induced milk fat depression (MFD) and to test the theory that these changes are related to milk fat fluidity. The experiment was divided into three periods: 1) Baseline: all cows (n = 12) received a high fiber diet without fish oil (FO) for 12 days; 2) Treatment: 4 cows/group received the following treatments for 21 days: a) Low fiber diet without FO (LF), b) High fiber diet + FO (HF + FO) and c) Low fiber diet + FO (LF + FO); 3) Post-treatment: cows returned to the baseline diet and were monitored for 12 days. FO was included at 1.6% DM and HF and LF diets had 40 and 26% NDF, respectively. Milk fat content and yield were unchanged by the LF diet, but were reduced by FO diets at both dietary fiber levels and recovered in the post-treatment period. FO diets caused a pronounced reduction in stearic and oleic acid concentrations in milk fat and an equally pronounced increase in trans-18:1 fatty acid concentrations. Milk fat mean melting point (MMP) was correlated with MFD (r = 0.73) and with milk oleic acid concentration (r = − 0.92). The ratio of oleic:stearic in milk fat increased gradually and consistently in response to FO. Trans-C18:1 isomers with double bounds at carbon ≤ 10 increased with greater MFD and those with double bonds at carbon ≥ 11 decreased with greater MFD. Trans-9 cis-11 CLA explained more than 80% of MFD and was strongly correlated with trans-10 C18:1. Maintenance of MMP below 39–40 °C suggests that the mammary gland was able to secrete only milk fat with adequate fluidity and that MFD could be an adaptation mechanism to prevent secretion of milk with higher MMP.
653 $aConjugated linoleic acids
653 $aFish oil
653 $aMilk fat depression
653 $aMilk fat fluidity
653 $aTrans-fatty acids
700 1 $aGARNSWORTHY, P. C.
700 1 $aGRIINARI, J. M.
700 1 $aLEME, P. R.
700 1 $aRODRIGUES, P. H. M.
700 1 $aSOUZA, L. W. O.
700 1 $aLANNA, D. P. D.
773 $tLivestock Science$gv. 115, n. 2/3, p. 319-331, 2008.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
24/11/2020 |
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Data da última atualização: |
24/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTOS, L. L.; SALGADO, J. A.; DRUMMOND, M. G.; BASTIANETTO, E.; SANTOS, C. P.; BRASIL, B. dos S. A. F.; TACONELI, C. A.; OLIVEIRA, D. A. A. |
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Afiliação: |
Lívia L. Santos, Universidade Federal de Minas Gerais; Jordana A. Salgado, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Marcela G. Drummond, Myleus Biotecnologia; Eduardo Bastianetto, Universidade Federal de Minas Gerais; Clóvis P. Santos, Universidade Estadual do Norte Fluminense Darcy Ribeiro; BRUNO DOS SANTOS A F BRASIL, SPD; Cesar A. Taconeli, Universidade Federal do Paraná; Denise A. A. Oliveira, Universidade Federal de Minas Gerais. |
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Título: |
Molecular method for the semiquantitative identification of gastrointestinal nematodes in domestic ruminants. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Parasitology Research, v. 119, p. 529-543, Feb. 2020. |
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DOI: |
https://doi.org/10.1007/s00436-019-06569-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Standard diagnostic methods currently in use for the identification of helminth infections in ruminants are based on the morphological analysis of immature and adult stages of parasites. This paper describes a method for the semiquantitative identification of nematodes, mainly Trichostrongyloidea, at species-level resolution. The method is based on amplification and fragment analysis followed by minisequencing of the ITS-2 region (internal transcribed spacer 2) of the ribosomal DNA of parasite eggs or larvae. This method allows for the identification of seven genera (Chabertia, Cooperia, Haemonchus, Oesophagostomum, Ostertagia, Teladorsagia, and Trichostrongylus) and 12 species (Chabertia ovina, Cooperia curticei, Cooperia punctata, Cooperia oncophora/Cooperia surnabada, Haemonchus contortus, Haemonchus placei, Haemonchus longistipes, Oesophagostomum asperum, Oesophagostomum radiatum, Ostertagia ostertagi, Trichostrongylus axei, and Trichostrongylus colubriformis) of infectious nematodes of domestic ruminants. The concordance between the morphological and molecular analyses in the detection of genera ranged from 0.84 to 0.99, suggesting the proposed detection method is specific, semiquantitative, less laborious, and highly cost-efficient. |
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Palavras-Chave: |
ITS-2; SNaPshot minisequencing. |
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Thesagro: |
Nematóide; Parasito de Animal; Parasitose; Ruminante. |
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Thesaurus NAL: |
Gastrointestinal nematodes; Ruminants; Veterinary parasitology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02249naa a2200325 a 4500
001 2127014
005 2020-11-24
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00436-019-06569-3$2DOI
100 1 $aSANTOS, L. L.
245 $aMolecular method for the semiquantitative identification of gastrointestinal nematodes in domestic ruminants.$h[electronic resource]
260 $c2020
520 $aStandard diagnostic methods currently in use for the identification of helminth infections in ruminants are based on the morphological analysis of immature and adult stages of parasites. This paper describes a method for the semiquantitative identification of nematodes, mainly Trichostrongyloidea, at species-level resolution. The method is based on amplification and fragment analysis followed by minisequencing of the ITS-2 region (internal transcribed spacer 2) of the ribosomal DNA of parasite eggs or larvae. This method allows for the identification of seven genera (Chabertia, Cooperia, Haemonchus, Oesophagostomum, Ostertagia, Teladorsagia, and Trichostrongylus) and 12 species (Chabertia ovina, Cooperia curticei, Cooperia punctata, Cooperia oncophora/Cooperia surnabada, Haemonchus contortus, Haemonchus placei, Haemonchus longistipes, Oesophagostomum asperum, Oesophagostomum radiatum, Ostertagia ostertagi, Trichostrongylus axei, and Trichostrongylus colubriformis) of infectious nematodes of domestic ruminants. The concordance between the morphological and molecular analyses in the detection of genera ranged from 0.84 to 0.99, suggesting the proposed detection method is specific, semiquantitative, less laborious, and highly cost-efficient.
650 $aGastrointestinal nematodes
650 $aRuminants
650 $aVeterinary parasitology
650 $aNematóide
650 $aParasito de Animal
650 $aParasitose
650 $aRuminante
653 $aITS-2
653 $aSNaPshot minisequencing
700 1 $aSALGADO, J. A.
700 1 $aDRUMMOND, M. G.
700 1 $aBASTIANETTO, E.
700 1 $aSANTOS, C. P.
700 1 $aBRASIL, B. dos S. A. F.
700 1 $aTACONELI, C. A.
700 1 $aOLIVEIRA, D. A. A.
773 $tParasitology Research$gv. 119, p. 529-543, Feb. 2020.
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