Data/hora: 02/06/2024 02:32:20

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 05/08/2016
Data da última atualização: | 16/05/2017
Autoria: | RIVAS, E. B.; BÔDI, E. C. de A.; HARAKAVA, R.; GREGORI, F.; GONÇALVES, M. C.
Afiliação: | ELIANA BORGES RIVAS, INSTITUTO BIOLÓGICO; ESTEVÃO CARDOSO DE ALMEIDA BÔDI; RICARDO HARAKAVA, IB/CPDSV; FÁBIO GREGORI, FMVZ/USP; MARCOS CESAR GONÇALVES, IB/CPDSV.
Título: | Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 5, p. 615-622, maio 2016.
Idioma: | Inglês
Notas: | Título em português: Ocorrência e análise molecular de vírus quarentenário em áreas de cultivo de lírios no Brasil.
Conteúdo: | The objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus ? LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT?PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV?infected lilies and nine fragments of 600 nt from LSV?infected lilies were sequenced. Phylogenetic tree reconstruction showed a robust branch containing the LMoV Brazilian sequences, other LMoV isolates, TBV, and Tulip band breaking virus, suggesting that all are LMoV isolates, although they are clustered into two subgroups. Phylogenetic analysis also showed a robust branch supporting all Brazilian and other LSV sequences, except for an LSV Japanese isolate. Recombination analyses also showed an LMoV recombinant isolate, whereas no recombination events were found among LSV isolates. Lily mottle virus is the prevalent virus in lily crops in Brazil, in single and mixed infections with LSV or Cucumber mosaic virus (CMV).
Palavras-Chave: | Análise de recombinação; Recombination analysis; RT-PCR.
Thesagro: | Filogenia.
Thesaurus Nal: | Lilium; Lily mottle virus; Lily symptomless virus; Phylogeny; Reverse transcriptase polymerase chain reaction.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/146133/1/Occurrence-and-molecular.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59845-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS