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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. |
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Afiliação: |
S. WOHLRES-VIANA, UFJF; E. K. N. ARASHIRO, UFF; T. P. MINARE, Universidade José do Rosario Vellano, Alfenas, MG; C. A. C. FERNANDES, Universidade Jose do Rosario Vellano / Biotran Biotecnologia Animal, Alfenas, MG; J. G. V. GRAZIA, UFMG; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Theriogenology, v. 126, p. 68-74, 2019. |
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DOI: |
10.1016/j.theriogenology.2018.12.004 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype. MenosAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production. |
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Thesaurus Nal: |
Embryo transfer; Luteinizing hormone. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02857naa a2200253 a 4500
001 2106166
005 2023-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.theriogenology.2018.12.004$2DOI
100 1 $aWOHLRES-VIANA, S.
245 $aDifferential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle.$h[electronic resource]
260 $c2019
520 $aAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype.
650 $aEmbryo transfer
650 $aLuteinizing hormone
653 $aIn vivo embryo production
700 1 $aARASHIRO, E. K. N.
700 1 $aMINARE, T. P.
700 1 $aFERNANDES, C. A. C.
700 1 $aGRAZIA, J. G. V.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aMACHADO, M. A.
700 1 $aVIANA, J. H. M.
773 $tTheriogenology$gv. 126, p. 68-74, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 21 | |
| 3. |  | GRÁZIA, J. G. V. de; BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; GARCIA, R. M. G.; IGUMA, L. T.; VIANA, J. H. M. Avaliação do acúmulo de lipídeos em oócitos de vacas Gir (Bos indicus) e Holandesa (Bos taurus). In: MOSTRA CIENTÍFICA, 16.; SEMANA DE BIOLOGIA DA UFJF, 33., 2010, Juiz de Fora. Anais... Juiz de Fora: UFJF/Dacbio, 2010. p. 43-46.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | AMARAL, T. F.; GRAZIA, J. G. V. de; MARTINHAO, L A. G.; DE COL, F.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; HANSEN, P. J. Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium. Scientific Reports, v. 12, 7503, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | BOLPATO, M. C. L.; SOUZA, E. D. de; GRÁZIA, J. G. V. de; ASSUNÇÃO, C. M. de; VIANA, J. H. M.; CAMARGO, L. S. de A. Efeito da raça e da estação do ano sobre a produção in vitro de embriões bovinos na região da Zona da Mata, MG. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | MARTINS, L. P.; MARTINHÃO, L. A. G.; GARCIA, I. N.; RIBAS, D. N.; GRÁZIA, J. G. V. de; FIGUEIREDO, R. A.; VIANA, J. H. M. Cryotolerance of in vitro produced bovine embryos originated from oocytes matured in medium supplemented with recombinant human FSH. Animal Reproduction, v. 19, n. 2, 2022. e22078. Proceedings of the 35rd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, August 2022. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; GRAZIA, J. G. V.; CAMARGO, L. S. de A.; MACHADO, M. A.; VIANA, J. H. M. Could the differential expression of luteinizing hormone receptor isoforms explain the varaiability in superovulatory responses in cattle? In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 41., 2015, Versailles. Reproductive performance: at the crossroads of genetics and the environment: abstracts. Versailles: IETS, 2015. p. 261.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | ASSUNÇÃO, C. M.; GRAZIA, J. G. V.; CAMPOS JR. P. H. A.; PEREIRA, M. M.; AZEVEDO, A. L. S.; QUINTÃO, C. C.; CAMARGO, L. S. de A. Efeito da mitomicina C sobre a viabilidade e ciclo celular de fibroblasto adultos bovinos após diferentes tempos de exposição. Acta Scientiae Veterinariae, v. 38, p. 401, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | GRÁZIA, J. G. V. de; ARASHIRO, E. K. N.; PALHAO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; VIANA, J. H. M. Is it possible to increase the efficiency of in vitro embryo production in Holstein preselecting donors with more oocyte production? Animal Reproduction, v.10, n.3, p. 526, Jul./Sept. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | GRAZIA, J. G. V.; LACERDA, L. G.; SIQUEIRA, L. G. B.; PELLEGRINO, C. A. G.; GRAPIUNA, L. S.; SOLLECITO, N. V.; VIANA, J. H. M. Effects of Gas Tension During Culture upon Development of Bovine Embryos from Beef (Nellore) and Dairy (Girolando) Breeds. In: International Embryo Technology Society and the Society for Reproductive Biology, v. 30 n. 1. Resumo em Anais. Bangkok, Tailandia. IETS, 2018. 207.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | GRÁZIA, J. G. V.; GOIS, A. C. N.; PELLEGRINO, A. P.; MARQUES-JÚNIOR, A. P.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Effect of in vitro maturation (IVM) method and gas tension during culture upon in vitro production of F1 embryos (Nelore x Angus) using Y-sorted semen. Animal Reproduction, v. 15, n. 3, p. 401, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | CARVALHO, B. C. de; BRANDÃO, F. Z.; SANTOS, G. B.; VARAGO, F. C.; OTTO, P. I.; VARGAS, M. W.; GRÁZIA, J. G. V.; CAMARGO, L. S. de A. Oocyte quality in post-partum of 3/4 and 7/8 Holstein x Zebu primiparous crossbred cows. Animal Reproduction, v. 10, n. 3, p. 559, Jul./Sept. 2013. Suplemento. Edição dos abstracts do 27º Annual Meeting of the Brazilian Embryo Technology Society, 2013, Praia do Forte, BA.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | POLISSENI, J.; SERAPIÃO, R. V.; CAMPOS JR. P. H. A.; GRAZIA, J. G. V. DE; MILEN, L. C.; CAMARGO, L. S. de A.; GUERRA, M. DE O.; PETERS, V. M. Tecnica de TUNEL em embriões de ratas Wistar: avaliação da qualidade e da capacidade de desenvolvimento dos embriões. Revista Interdisciplinar de Estudos Experimentais, v. 1, n. 3, p. 110-113, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | ARASHIRO, E. K. N.; GRAZIA, J. G. V.; SANTOS, J. L. R.; BELMONTE, S. L. R.; PALHÃO, M. P.; FERNANDES, C. A. C.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Use of three-dimensional models as an alternative to evalue body condition in cattle: preliminary results. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 28., 2014, Natal. Proceedings...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | CARVALHO, B. C. de; SANTOS, G. B.; BRANDÃO, F. Z.; VARAGO, F. C.; VARGAS, M. W.; CAMARGO, L. S. de A.; MACHADO, F. S.; CAMPOS, M. M.; GRAZIA, J. G. V. Follicular concentration of IGF-I and insulin in the postpartum of girolando crossbred cows. Animal Reproduction, v. 11, n. 3, p. 451, 2014. Edição dos proceedings do 28º Annual Meeting of the Brazilian Embryo Technology Society, 2014, Natal.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. Theriogenology, v. 126, p. 68-74, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | ASSUNÇÃO, C. M.; GRAZIA, J. G. V.; CAMPOS JR. P. H. A.; PEREIRA, M. M.; AZEVEDO, A. L. S.; QUINTAO, C. C. R.; VIANA, J. H. M.; CAMARGO, L. S. de A. Effect of mitomycin C on viability and cell cycle of bovine adult fibroblasts after different exposure times. Acta Scientiae Veterinariae, v. 38, p. s797, 2010. Supl.2.
Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 20. | | AMARAL, T. F.; GRÁZIA, J. G. V.; GONELLA-DIAZA, A. M.; MARTINHÃO, L. A. G.; HEREDIA, D.; MELO, G. D.; POHLER, K. G.; ESTRADA-CORTÉS, E.; DIKMEN, S.; SOSA, F.; JENSEN, L. M.; SANG, L.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; HANSEN, P. J. Actions of DKK1 on the bovine embryo during the morula-to-blastocyst stage of development on pregnancy outcomes and placental hormone secretion after embryo transfer. Reproduction, Fertility and Development, v. 34, n. 2, p. 279, 2022. Edição dos abstracts da Annual Conference of the International Embryo Transfer Society, 48., Savannah, Geórgia, 2022.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 21 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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