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| 46. |  | CARPANEZZI, A. A.; ZANON, A.; IEDE, E. T.; STURION, J. A.; GRACA, M. E. C.; LOURENCO, R. S. Diretrizes de pesquisa aplicada para plantios de erva-mate no Brasil. In: CONGRESSO FLORESTAL DO PARANA, 2., 1988, Curitiba. Anais dos resumos. Curitiba: Instituto Florestal do Parana, 1988. p.59.| Biblioteca(s): Embrapa Florestas. |
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| 47. | | CARPANEZZI, A. A.; CARDOSO, A.; VALIO, I. F. M.; GRACA, M. E. C.; IEDE, E. T.; HIGA, R. C. V. Queda anormal de folhas de erva-mate (Ilex paraguariensis St. Hil.) em 1983. In: SEMINÁRIO SOBRE ATUALIDADES E PERSPECTIVAS FLORESTAIS, 10., 1983, Curitiba. Silvicultura da erva-mate (Ilex paraguariensis): anais... Curitiba: EMBRAPA-CNPF, 1985. p. 141-145. (EMBRAPA-CNPF. Documentos, 15).| Biblioteca(s): Embrapa Florestas. |
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| 48. | | MEDEIROS, A. C. de S.; ABREU, D. C. A. de; BOSCARDIM, D. M.; GRAÇA, M. E. C.; KALIL FILHO, A. N. Efeitos do substrato, temperatura e da água na germinação de sementes de Escallonia montevidensis Cham. & Schl. Dc. In: CONGRESSO NACIONAL DE BOTÂNICA, 51., 2000, Brasília. Resumos. Brasília: Sociedade Botânica do Brasil, 2000. p.73. T0231. Resumo.| Biblioteca(s): Embrapa Florestas. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/08/2021 |
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Data da última atualização: |
11/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
LI, Y.; TRÍBULO, P.; BAKHTIARIZADEH, M. R.; SIQUEIRA, L. G. B.; JI, T.; RIVERA, R. M.; HANSEN, P. J. |
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Afiliação: |
YAHAN LI, University of Missouri; PAULA TRÍBULO, Instituto de Reproduccion Animal Córdoba; MOHAMMAD REZA BAKHTIARIZADEH, University of Tehran, Iran; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; TIEMING JI, University of Missouri; ROCÍO MELISSA RIVERA, University of Missouri; PETER JAMES HANSEN, University of Florida. |
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Título: |
Conditions of embryo culture from days 5 to 7 of development alter the DNA methylome of the bovine fetus at day 86 of gestation. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Assisted Reproduction and Genetics, v. 37, p. 417-426, 2020. |
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DOI: |
https://doi.org/10.1007/s10815-019-01652-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Purpose: We tested whether in vitro production (IVP) causes changes in DNAmethylation in fetal liver and skeletalmuscle and if exposure of cultured embryos to colony-stimulating factor 2 (CSF2) alters DNA methylation. Methods: Female fetuses were produced by artificial insemination or transfer of an IVP embryo. Embryos were treated fromdays 5 to 7 after fertilization with CSF2 or vehicle. DNA methylation in fetal liver and skeletal muscle was determined by postbisulfite adaptor tagging-based sequencing. The degree of DNA methylation for CpG sites in 50-bp windows of the promoter region 500 bp upstream of the transcriptional start site was compared between treatments. Results For liver, there were 12 genes (6%of those analyzed) in which DNA methylation was affected by treatment, with one 50-bp window per gene affected by treatment. For muscle, the degree ofDNAmethylation was affected by treatment for 32windows(19% of the total windows analyzed) representing 28 distinct genes (23% of analyzed genes). For 19 of the 28 genes in muscle, the greatest deviation in DNA methylation was for the CSF2 group. Conclusion: Results are consistent with alterations in the methylome being one of the mechanisms by which IVP can result in altered fetal development and postnatal function in the resultant offspring. In addition, results indicate that maternally derived cell-signaling molecules can regulate the pattern of DNA methylation. |
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Palavras-Chave: |
Fertilização in vitro; Programação fetal. |
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Thesagro: |
Bovino; DNA; Embrião Animal; Metilação; Reprodução Animal. |
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Thesaurus NAL: |
DNA methylation; In vitro fertilization. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02412naa a2200313 a 4500
001 2133460
005 2021-08-11
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10815-019-01652-1$2DOI
100 1 $aLI, Y.
245 $aConditions of embryo culture from days 5 to 7 of development alter the DNA methylome of the bovine fetus at day 86 of gestation.$h[electronic resource]
260 $c2020
520 $aPurpose: We tested whether in vitro production (IVP) causes changes in DNAmethylation in fetal liver and skeletalmuscle and if exposure of cultured embryos to colony-stimulating factor 2 (CSF2) alters DNA methylation. Methods: Female fetuses were produced by artificial insemination or transfer of an IVP embryo. Embryos were treated fromdays 5 to 7 after fertilization with CSF2 or vehicle. DNA methylation in fetal liver and skeletal muscle was determined by postbisulfite adaptor tagging-based sequencing. The degree of DNA methylation for CpG sites in 50-bp windows of the promoter region 500 bp upstream of the transcriptional start site was compared between treatments. Results For liver, there were 12 genes (6%of those analyzed) in which DNA methylation was affected by treatment, with one 50-bp window per gene affected by treatment. For muscle, the degree ofDNAmethylation was affected by treatment for 32windows(19% of the total windows analyzed) representing 28 distinct genes (23% of analyzed genes). For 19 of the 28 genes in muscle, the greatest deviation in DNA methylation was for the CSF2 group. Conclusion: Results are consistent with alterations in the methylome being one of the mechanisms by which IVP can result in altered fetal development and postnatal function in the resultant offspring. In addition, results indicate that maternally derived cell-signaling molecules can regulate the pattern of DNA methylation.
650 $aDNA methylation
650 $aIn vitro fertilization
650 $aBovino
650 $aDNA
650 $aEmbrião Animal
650 $aMetilação
650 $aReprodução Animal
653 $aFertilização in vitro
653 $aProgramação fetal
700 1 $aTRÍBULO, P.
700 1 $aBAKHTIARIZADEH, M. R.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aJI, T.
700 1 $aRIVERA, R. M.
700 1 $aHANSEN, P. J.
773 $tJournal of Assisted Reproduction and Genetics$gv. 37, p. 417-426, 2020.
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