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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
07/08/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
MOURA, J. B.; VARGAS, A. C. de; GOUVEIA, G. V.; GOUVEIA, J. J. de S.; RAMOS-JÚNIOR, J. C.; BOTTON, S. de A.; PEREIRA, E. C.; COSTA, M. M. da. |
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Afiliação: |
Jusciêne B. Moura, Universidade Federal do Vale do São Francisco - Univasf/Laboratório de Microbiologia e Imunologia Animal; Agueda C. de Vargas, Universidade Federal de Santa Maria/UFSM/Laboratório de Bacteriologia - Labac, Centro de Ciências Rurais/Departamento de Medicina Veterinária Preventiva.; Gisele V. Gouveia, Universidade Federal do Vale do São Francisco - Univasf/Laboratório de Microbiologia e Imunologia Animal; João J. de S. Gouveia, Universidade Federal do Vale do São Francisco - Univasf/Laboratório de Microbiologia e Imunologia Animal; Juracy C. Ramos-Júnior, Clínica Veterinária São Francisco - Clivesf; Sônia de A. Botton, Universidade Federal de Santa Maria/UFSM/Laboratório de Bacteriologia - Labac, Centro de Ciências Rurais/Departamento de Medicina Veterinária Preventiva.; Eugênia C. Pereira, Universidade Federal de Pernambuco - UFPE,Departamento de Ciências Geográficas, Centro de Filosofia e Ciências Humanas; Mateus M. da Costa, Universidade Federal do Vale do São Francisco - Univasf/Laboratório de Microbiologia e Imunologia Animal. |
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Título: |
In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 4, p. 368-378, abril 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Atividade antimicrobiana in vitro do extrato orgânico de Cladonia substellata Vainio e ácido úsnico frente Staphylococcus spp. obtidos de cães e gatos. |
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Conteúdo: |
Cladonia substellata Vainio is a lichen found in different regions of the world, including the Northeast of Brazil. It contains several secondary metabolites with biological activity, including usnic acid, which has exhibited a wide range of biological activities. The aim of this study was to evaluate the in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid. Initially, Staphylococcus spp., derived from samples of skin and ears of dogs and cats with suspected pyoderma and otitis, were isolated and analyzed. In antimicrobial susceptibility testing against Staphylococcus spp., 77% (105/136) of the isolates were resistant to the antimicrobials tested. In the assessment of biofilm production, 83% (113/136) were classified as producing biofilm. In genetic characterization, 32% (44/136) were positive for blaZ, no isolate (0/136) was positive for the mecA gene, and 2% (3/136) were positive for the icaD gene. The in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid against Staphylococcus spp. ranged from 0.25mg/mL to 0.0019mg/mL, inhibiting bacterial growth at low concentrations. The substances were more effective against biofilm-producing bacteria (0.65mg/mL-0.42mg/mL) when compared to non-biofilm producing bacteria (2.52mg/mL-2.71mg/mL). Usnic acid and the organic extract of C. substellata can be effective in the treatment of pyoderma and otitis in dogs and cats caused by Staphylococcus spp. |
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Palavras-Chave: |
Antimicrobial activity; Antimicrobiano; Cladonia substellata; Lichen. |
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Thesagro: |
Bactéria; Biofilme; Cão; Gato; Gene. |
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Thesaurus Nal: |
Biofilm; Cats; Dogs; Genes; Lichens; Usnic acid. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162500/1/In-vitro-atimicrobial-activity.pdf
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Marc: |
LEADER 02796naa a2200397 a 4500
001 2073707
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aMOURA, J. B.
245 $aIn vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Atividade antimicrobiana in vitro do extrato orgânico de Cladonia substellata Vainio e ácido úsnico frente Staphylococcus spp. obtidos de cães e gatos.
520 $aCladonia substellata Vainio is a lichen found in different regions of the world, including the Northeast of Brazil. It contains several secondary metabolites with biological activity, including usnic acid, which has exhibited a wide range of biological activities. The aim of this study was to evaluate the in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid. Initially, Staphylococcus spp., derived from samples of skin and ears of dogs and cats with suspected pyoderma and otitis, were isolated and analyzed. In antimicrobial susceptibility testing against Staphylococcus spp., 77% (105/136) of the isolates were resistant to the antimicrobials tested. In the assessment of biofilm production, 83% (113/136) were classified as producing biofilm. In genetic characterization, 32% (44/136) were positive for blaZ, no isolate (0/136) was positive for the mecA gene, and 2% (3/136) were positive for the icaD gene. The in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid against Staphylococcus spp. ranged from 0.25mg/mL to 0.0019mg/mL, inhibiting bacterial growth at low concentrations. The substances were more effective against biofilm-producing bacteria (0.65mg/mL-0.42mg/mL) when compared to non-biofilm producing bacteria (2.52mg/mL-2.71mg/mL). Usnic acid and the organic extract of C. substellata can be effective in the treatment of pyoderma and otitis in dogs and cats caused by Staphylococcus spp.
650 $aBiofilm
650 $aCats
650 $aDogs
650 $aGenes
650 $aLichens
650 $aUsnic acid
650 $aBactéria
650 $aBiofilme
650 $aCão
650 $aGato
650 $aGene
653 $aAntimicrobial activity
653 $aAntimicrobiano
653 $aCladonia substellata
653 $aLichen
700 1 $aVARGAS, A. C. de
700 1 $aGOUVEIA, G. V.
700 1 $aGOUVEIA, J. J. de S.
700 1 $aRAMOS-JÚNIOR, J. C.
700 1 $aBOTTON, S. de A.
700 1 $aPEREIRA, E. C.
700 1 $aCOSTA, M. M. da
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 4, p. 368-378, abril 2017.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
16/12/2013 |
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Data da última atualização: |
10/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PEREIRA, G. S.; NUNES, E. S.; LAPERUTA, L. D. C.; BRAGA, M. F.; PENHA, H. A.; DINIZ, A. L.; MUNHOZ, C. F.; GAZAFFI, R.; GARCIA, A. A. F.; VIEIRA, M. L. C. |
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Afiliação: |
ESALQ/USP; ESALQ/USP; ESALQ/USP; MARCELO FIDELES BRAGA, CPAC; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP. |
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Título: |
Molecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Annals of Applied Biology, v. 162, n. 3, p. 347-361, 2013. |
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ISSN: |
0003-4746 |
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DOI: |
10.1111/aab.12028 |
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Idioma: |
Inglês |
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Conteúdo: |
One of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0 cM, with an average distance between adjacent markers of 3.8 cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping. MenosOne of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of w... Mostrar Tudo |
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Thesagro: |
Maracujá; Marcador genético; Marcador molecular; Melhoramento genético vegetal; Polimorfismo Genético. |
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Thesaurus NAL: |
Genetic improvement; Genetic markers; Genetic polymorphism; Passiflora alata; Passion fruits. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02903naa a2200373 a 4500
001 1973873
005 2014-12-10
008 2013 bl uuuu u00u1 u #d
022 $a0003-4746
024 7 $a10.1111/aab.12028$2DOI
100 1 $aPEREIRA, G. S.
245 $aMolecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species.
260 $c2013
520 $aOne of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0 cM, with an average distance between adjacent markers of 3.8 cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping.
650 $aGenetic improvement
650 $aGenetic markers
650 $aGenetic polymorphism
650 $aPassiflora alata
650 $aPassion fruits
650 $aMaracujá
650 $aMarcador genético
650 $aMarcador molecular
650 $aMelhoramento genético vegetal
650 $aPolimorfismo Genético
700 1 $aNUNES, E. S.
700 1 $aLAPERUTA, L. D. C.
700 1 $aBRAGA, M. F.
700 1 $aPENHA, H. A.
700 1 $aDINIZ, A. L.
700 1 $aMUNHOZ, C. F.
700 1 $aGAZAFFI, R.
700 1 $aGARCIA, A. A. F.
700 1 $aVIEIRA, M. L. C.
773 $tAnnals of Applied Biology$gv. 162, n. 3, p. 347-361, 2013.
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