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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
28/11/2017 |
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Data da última atualização: |
28/11/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MENDES, N. L.; BRAGA, E. C. de O.; SANTIAGO, M. C. P. de A.; NASCIMENTO, L. da S. de M. do; PACHECO, S.; BORGUINI, R. G.; GOUVÊA, A. C. M. S.; GODOY, R. L. de O. |
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Afiliação: |
Nathália Leal Mendes, UFRRJ; Elaine Cristina de Oliveira Braga, UFRJ; MANUELA CRISTINA P DE A SANTIAGO, CTAA; LUZIMAR DA SILVA DE M DO NASCIMENTO, CTAA; SIDNEY PACHECO, CTAA; RENATA GALHARDO BORGUINI, CTAA; Ana Cristina Miranda Senna Gouvêa; RONOEL LUIZ DE OLIVEIRA GODOY, CTAA. |
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Título: |
Evaluation of influence of two different drying methods in the phenolic content of jamelão fruit peel. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS , 12., 2017, Campinas. Ciência de Alimentos e seu Impacto no Mundo em Transformação. Campinas: UNICAMP, 2017. |
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Idioma: |
Inglês |
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Notas: |
SLACA, 12. De 4 a 7 de Novembro de 2017. |
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Conteúdo: |
The jamelão (Syzygium cumini (L) Skeels.) fruit properties are generally related to the antioxidant activity of the phenolic compounds present in its composition. Flavonoids, mainly anthocyanins, and phenolic acids are the major compounds present in this fruit. The aim of this work was to compare the influence of two drying methods (convective drying and freeze drying) in the phenolic content of jamelão fruit peel. Jamelão fruits were collected at Guaratiba, located in Rio de Janeiro, RJ. The analysed samples were: in natura peel (frozen), dried peel (T = 60 ° C for 20 h) and freeze- dried peel. After drying, the material was ground and sieved (Mesh 48). The analyzes were done in triplicate, using 1.0 g of sample. The extraction was done in two stages, the first one using methanol and acetone to obtain free phenolics, and a second stage of hydrolysis, to obtain hydrolyzable phenolics. The profile analysis was performed by High Performance Liquid Chromatography with a photodiode array detector. The phenolic substances found in all extractions were: rutin and quercetin, respectively, 0.0128 mg.g-1 and 0.081 mg.g-1 for the in natura peel, 0.115 mg.g-1 and 0.082 mg.g-1 for the dried peel, and 0.142 mg.g-1 and 0.088 mg.g-1 for the freeze dried peel. Luteolin was found in the dried peel (0.146 mg.g-1), and the values were slightly higher than those found for freeze-dried (0.129 mg.g.g-1) and in natura peel (0.132 mg.g-1) . After hydrolysis step, gallic and p-coumaric acids were found (6,630 mg.g-1 and 0,074 mg.g-1, respectively) in the dried peel and freeze-dried peel (4,179 mg.g-1 and 0,056 mg.g-1). These compounds were not detected in the in natura peel. In general, jamelão dried peel presented larger amounts of phenolics substances than the freeze-dried peel. MenosThe jamelão (Syzygium cumini (L) Skeels.) fruit properties are generally related to the antioxidant activity of the phenolic compounds present in its composition. Flavonoids, mainly anthocyanins, and phenolic acids are the major compounds present in this fruit. The aim of this work was to compare the influence of two drying methods (convective drying and freeze drying) in the phenolic content of jamelão fruit peel. Jamelão fruits were collected at Guaratiba, located in Rio de Janeiro, RJ. The analysed samples were: in natura peel (frozen), dried peel (T = 60 ° C for 20 h) and freeze- dried peel. After drying, the material was ground and sieved (Mesh 48). The analyzes were done in triplicate, using 1.0 g of sample. The extraction was done in two stages, the first one using methanol and acetone to obtain free phenolics, and a second stage of hydrolysis, to obtain hydrolyzable phenolics. The profile analysis was performed by High Performance Liquid Chromatography with a photodiode array detector. The phenolic substances found in all extractions were: rutin and quercetin, respectively, 0.0128 mg.g-1 and 0.081 mg.g-1 for the in natura peel, 0.115 mg.g-1 and 0.082 mg.g-1 for the dried peel, and 0.142 mg.g-1 and 0.088 mg.g-1 for the freeze dried peel. Luteolin was found in the dried peel (0.146 mg.g-1), and the values were slightly higher than those found for freeze-dried (0.129 mg.g.g-1) and in natura peel (0.132 mg.g-1) . After hydrolysis step, gallic and p-coumaric acids were fo... Mostrar Tudo |
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Palavras-Chave: |
HPLC- PDA; Phenolics. |
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Thesagro: |
Myrtaceae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02676nam a2200241 a 4500
001 2080957
005 2017-11-28
008 2017 bl uuuu u00u1 u #d
100 1 $aMENDES, N. L.
245 $aEvaluation of influence of two different drying methods in the phenolic content of jamelão fruit peel.$h[electronic resource]
260 $aIn: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS , 12., 2017, Campinas. Ciência de Alimentos e seu Impacto no Mundo em Transformação. Campinas: UNICAMP$c2017
500 $aSLACA, 12. De 4 a 7 de Novembro de 2017.
520 $aThe jamelão (Syzygium cumini (L) Skeels.) fruit properties are generally related to the antioxidant activity of the phenolic compounds present in its composition. Flavonoids, mainly anthocyanins, and phenolic acids are the major compounds present in this fruit. The aim of this work was to compare the influence of two drying methods (convective drying and freeze drying) in the phenolic content of jamelão fruit peel. Jamelão fruits were collected at Guaratiba, located in Rio de Janeiro, RJ. The analysed samples were: in natura peel (frozen), dried peel (T = 60 ° C for 20 h) and freeze- dried peel. After drying, the material was ground and sieved (Mesh 48). The analyzes were done in triplicate, using 1.0 g of sample. The extraction was done in two stages, the first one using methanol and acetone to obtain free phenolics, and a second stage of hydrolysis, to obtain hydrolyzable phenolics. The profile analysis was performed by High Performance Liquid Chromatography with a photodiode array detector. The phenolic substances found in all extractions were: rutin and quercetin, respectively, 0.0128 mg.g-1 and 0.081 mg.g-1 for the in natura peel, 0.115 mg.g-1 and 0.082 mg.g-1 for the dried peel, and 0.142 mg.g-1 and 0.088 mg.g-1 for the freeze dried peel. Luteolin was found in the dried peel (0.146 mg.g-1), and the values were slightly higher than those found for freeze-dried (0.129 mg.g.g-1) and in natura peel (0.132 mg.g-1) . After hydrolysis step, gallic and p-coumaric acids were found (6,630 mg.g-1 and 0,074 mg.g-1, respectively) in the dried peel and freeze-dried peel (4,179 mg.g-1 and 0,056 mg.g-1). These compounds were not detected in the in natura peel. In general, jamelão dried peel presented larger amounts of phenolics substances than the freeze-dried peel.
650 $aMyrtaceae
653 $aHPLC- PDA
653 $aPhenolics
700 1 $aBRAGA, E. C. de O.
700 1 $aSANTIAGO, M. C. P. de A.
700 1 $aNASCIMENTO, L. da S. de M. do
700 1 $aPACHECO, S.
700 1 $aBORGUINI, R. G.
700 1 $aGOUVÊA, A. C. M. S.
700 1 $aGODOY, R. L. de O.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Milho e Sorgo. |
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Data corrente: |
23/11/2011 |
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Data da última atualização: |
28/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RUIZ, J. C.; D'AFONSECA, V.; SILVA, A.; ALI, A.; PINTO, A. C.; SANTOS, A. R.; ROCHA, A. A. M. C.; LOPES, D. O.; DORELLA, F. A.; PACHECO, L. G. C.; COSTA, M. P.; TURK, M. Z.; SEYFFERT, N.; MORAES, P. M. R. O.; SOARES, S. C.; ALMEIDA, S. S.; CASTRO, T. L. P.; ABREU, V. A. C.; TROST, E.; BAUMBACH, J.; TAUCH, A.; SCHNEIDER, M. P. C.; McCULLOCH, J.; CERDEIRA, L. T.; RAMOS, R. T. J.; ZERLOTINI, A.; DOMINITINI, A.; RESENDE, D. M.; COSER, E. M.; OLIVEIRA, L. M.; PEDROSA, A. L.; VIEIRA, C. U.; GUIMARAES, C. T.; BARTHOLOMEU, D. C.; OLIVEIRA, D. M.; SANTOS, F. R.; RABELO, E. M.; LOBO, F. P.; FRANCO, G. R.; COSTA, A. F.; CASTRO, I. M.; DIAS, S. R. C.; FERRO, J. A.; ORTEGA, J. M.; PAIVA, L. V.; ALMEIDA, J. F.; GOULART, L. R.; FERRO, M. I. T.; CARNEIRO, N. P.; FALCÃO, P. R. K.; GRYNBERG, P.; TEIXEIRA, S. M. R.; BROMMONSCHENKEL, S.; OLIVEIRA, S. C.; MEYER, R.; MOORE, R. J.; MIYOSHI, A.; OLIVEIRA, G. C.; AZEVEDO, V. |
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Afiliação: |
CLAUDIA TEIXEIRA GUIMARAES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS. |
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Título: |
Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Plos One, San Francisco, v. 6, n. 4, p. 1-16, 2011. |
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DOI: |
10.1371/journal.pone.0018551 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity. Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal transfer. Conclusions: These particular genome characteristics of C. pseudotuberculosis, as well as its acquired virulence factors in pathogenicity islands, provide evidence of its lifestyle and of the pathogenicity pathways used by this pathogen in the infection process. All genomes cited in this study are available in the NCBI Genbank database (http://www.ncbi.nlm.nih.gov/genbank/) under accession numbers CP001809 and CP001829. MenosBackground: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity. Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal t... Mostrar Tudo |
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Palavras-Chave: |
Análise de genoma; Genoma de C pseudotuberculosis; Ilhas de patogenicidade. |
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Thesagro: |
Corynebacterium Pseudotuberculosis; Genética; Patógeno. |
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Thesaurus NAL: |
Caseous lymphadenitis; Genome; Pathogenicity. |
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Categoria do assunto: |
--
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/47474/1/Evidence-reductive.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53621/1/journal.pone.0018551.pdf
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Marc: |
LEADER 04503naa a2200937 a 4500
001 1906799
005 2017-09-28
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0018551$2DOI
100 1 $aRUIZ, J. C.
245 $aEvidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.$h[electronic resource]
260 $c2011
520 $aBackground: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity. Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal transfer. Conclusions: These particular genome characteristics of C. pseudotuberculosis, as well as its acquired virulence factors in pathogenicity islands, provide evidence of its lifestyle and of the pathogenicity pathways used by this pathogen in the infection process. All genomes cited in this study are available in the NCBI Genbank database (http://www.ncbi.nlm.nih.gov/genbank/) under accession numbers CP001809 and CP001829.
650 $aCaseous lymphadenitis
650 $aGenome
650 $aPathogenicity
650 $aCorynebacterium Pseudotuberculosis
650 $aGenética
650 $aPatógeno
653 $aAnálise de genoma
653 $aGenoma de C pseudotuberculosis
653 $aIlhas de patogenicidade
700 1 $aD'AFONSECA, V.
700 1 $aSILVA, A.
700 1 $aALI, A.
700 1 $aPINTO, A. C.
700 1 $aSANTOS, A. R.
700 1 $aROCHA, A. A. M. C.
700 1 $aLOPES, D. O.
700 1 $aDORELLA, F. A.
700 1 $aPACHECO, L. G. C.
700 1 $aCOSTA, M. P.
700 1 $aTURK, M. Z.
700 1 $aSEYFFERT, N.
700 1 $aMORAES, P. M. R. O.
700 1 $aSOARES, S. C.
700 1 $aALMEIDA, S. S.
700 1 $aCASTRO, T. L. P.
700 1 $aABREU, V. A. C.
700 1 $aTROST, E.
700 1 $aBAUMBACH, J.
700 1 $aTAUCH, A.
700 1 $aSCHNEIDER, M. P. C.
700 1 $aMcCULLOCH, J.
700 1 $aCERDEIRA, L. T.
700 1 $aRAMOS, R. T. J.
700 1 $aZERLOTINI, A.
700 1 $aDOMINITINI, A.
700 1 $aRESENDE, D. M.
700 1 $aCOSER, E. M.
700 1 $aOLIVEIRA, L. M.
700 1 $aPEDROSA, A. L.
700 1 $aVIEIRA, C. U.
700 1 $aGUIMARAES, C. T.
700 1 $aBARTHOLOMEU, D. C.
700 1 $aOLIVEIRA, D. M.
700 1 $aSANTOS, F. R.
700 1 $aRABELO, E. M.
700 1 $aLOBO, F. P.
700 1 $aFRANCO, G. R.
700 1 $aCOSTA, A. F.
700 1 $aCASTRO, I. M.
700 1 $aDIAS, S. R. C.
700 1 $aFERRO, J. A.
700 1 $aORTEGA, J. M.
700 1 $aPAIVA, L. V.
700 1 $aALMEIDA, J. F.
700 1 $aGOULART, L. R.
700 1 $aFERRO, M. I. T.
700 1 $aCARNEIRO, N. P.
700 1 $aFALCÃO, P. R. K.
700 1 $aGRYNBERG, P.
700 1 $aTEIXEIRA, S. M. R.
700 1 $aBROMMONSCHENKEL, S.
700 1 $aOLIVEIRA, S. C.
700 1 $aMEYER, R.
700 1 $aMOORE, R. J.
700 1 $aMIYOSHI, A.
700 1 $aOLIVEIRA, G. C.
700 1 $aAZEVEDO, V.
773 $tPlos One, San Francisco$gv. 6, n. 4, p. 1-16, 2011.
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