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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
17/02/2023 |
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Data da última atualização: |
01/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BASTOS, B. D. M.; SILVA, N. P. da S.; GONÇALVES, P. R.; CANDIDO, A. E. C. M.; BARBERINO, R. de S.; MONTE, A. P. O. do; NOGUEIRA, D. M.; CORDEIRO, M. F.; MATOS, M. H. T. de; LOPES JUNIOR, E. S. |
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Afiliação: |
BRUNA DIAS MANGUEIRA BASTOS, UNIVASF; MARIA NAIARA PEREIRA DA SILVA, UNIVASF; PRICILA RAMOS GONÇALVES, UNIVASF; ANA ELISA CUSTÓDIO MONTES CÂNDIDO, UNIVASF; RICÁSSIO DE SOUSA BARBERINO, UNIVASF; ALANE PAINS OLIVEIRA DO MONTE, UNIVASF; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, UNIVASF; MARIA HELENA TAVARES DE MATOS, UNIVASF; EDILSON SOARES LOPES JÚNIOR, UNIVASF. |
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Título: |
Effect of different gonadotropins on in vitro maturation of sheep oocytes. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, v. 43, N. 6, p. 2731-2742, nov./dez. 2022. |
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ISSN: |
1676-546X |
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DOI: |
https://doi.org/10.5433/1679-0359.2022v43n6p2731 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes. |
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Palavras-Chave: |
GSH; Hoechst 33342; Hormônio folículo estimulante; Metafase II; Oócitos ovinos. |
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Thesagro: |
Hormônio Animal; Maturação Artificial; Ovino. |
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Thesaurus Nal: |
Follicle-stimulating hormone; Metaphase; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1151836/1/Efeito-de-diferentes-gonadotrofinas-na-maturacao.-2022.pdf
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Marc: |
LEADER 02307naa a2200385 a 4500
001 2151836
005 2023-03-01
008 2022 bl uuuu u00u1 u #d
022 $a1676-546X
024 7 $ahttps://doi.org/10.5433/1679-0359.2022v43n6p2731$2DOI
100 1 $aBASTOS, B. D. M.
245 $aEffect of different gonadotropins on in vitro maturation of sheep oocytes.$h[electronic resource]
260 $c2022
520 $aThe aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes.
650 $aFollicle-stimulating hormone
650 $aMetaphase
650 $aSheep
650 $aHormônio Animal
650 $aMaturação Artificial
650 $aOvino
653 $aGSH
653 $aHoechst 33342
653 $aHormônio folículo estimulante
653 $aMetafase II
653 $aOócitos ovinos
700 1 $aSILVA, N. P. da S.
700 1 $aGONÇALVES, P. R.
700 1 $aCANDIDO, A. E. C. M.
700 1 $aBARBERINO, R. de S.
700 1 $aMONTE, A. P. O. do
700 1 $aNOGUEIRA, D. M.
700 1 $aCORDEIRO, M. F.
700 1 $aMATOS, M. H. T. de
700 1 $aLOPES JUNIOR, E. S.
773 $tSemina: Ciências Agrárias$gv. 43, N. 6, p. 2731-2742, nov./dez. 2022.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
22/04/2016 |
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Data da última atualização: |
22/04/2016 |
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Autoria: |
BORSANELLI, A. C.; GAETTI-JARDIM JÚNIOR, E.; SCHWEITZER, C. M.; DOBEREINER, J.; DUTRA, I. S. |
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Afiliação: |
ANA CAROLINA BORSANELLI, UNESP; ELERSON GAETTI-JARDIM JÚNIOR, UNESP; CHRISTIANE MARIE SCHWEITZER, UNESP; JURGEN DOBEREINER; IVERALDO S. DUTRA, UNESP. |
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Título: |
Persence of porphyromonas and prevotella species in the oral microflora of cattle with periodontitis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 10, p. 829-834, out. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Bovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26) and in gingival sulcus of animals considered periodontally healthy (n=25). Presence of the microorganisms was evaluated by independent- culture medium diagnostic method, using polymerase chain reaction (PCR) with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%), P. melaninogenica (73.1%) and P. intermedia (61.5%) were the most predominant in samples of cattle with periodontitis. Regarding non-injured gingival sulcus of cattle, P. endodontalis (40%) and P. loeschei (40%) prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003), P. endodontalis (p=0.0023), Prevotella buccae (p=0.0017), P. intermedia (p=0.0020), P. melaninogenica (p=0.00006) and P. oralis (p=0.0028) is correlated with bovine periodontitis. |
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Palavras-Chave: |
Periodontite bovina; Porphyromonas spp; Prevotella spp. |
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Thesagro: |
Gado. |
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Thesaurus NAL: |
Cattle; Periodontitis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142498/1/Presence-of-Porphyromonas.pdf
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Marc: |
LEADER 02190naa a2200241 a 4500
001 2043797
005 2016-04-22
008 2015 bl uuuu u00u1 u #d
100 1 $aBORSANELLI, A. C.
245 $aPersence of porphyromonas and prevotella species in the oral microflora of cattle with periodontitis.
260 $c2015
520 $aBovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26) and in gingival sulcus of animals considered periodontally healthy (n=25). Presence of the microorganisms was evaluated by independent- culture medium diagnostic method, using polymerase chain reaction (PCR) with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%), P. melaninogenica (73.1%) and P. intermedia (61.5%) were the most predominant in samples of cattle with periodontitis. Regarding non-injured gingival sulcus of cattle, P. endodontalis (40%) and P. loeschei (40%) prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003), P. endodontalis (p=0.0023), Prevotella buccae (p=0.0017), P. intermedia (p=0.0020), P. melaninogenica (p=0.00006) and P. oralis (p=0.0028) is correlated with bovine periodontitis.
650 $aCattle
650 $aPeriodontitis
650 $aGado
653 $aPeriodontite bovina
653 $aPorphyromonas spp
653 $aPrevotella spp
700 1 $aGAETTI-JARDIM JÚNIOR, E.
700 1 $aSCHWEITZER, C. M.
700 1 $aDOBEREINER, J.
700 1 $aDUTRA, I. S.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 10, p. 829-834, out. 2015.
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Embrapa Unidades Centrais (AI-SEDE) |
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