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Registro Completo |
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
Data corrente: |
11/02/2015 |
Data da última atualização: |
22/02/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; SILVA, O. F.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. S.; BITTENCOURT, D. M. C.; MILLER, R. N. G. |
Afiliação: |
GLAUCIA E. O. MIDORIKAWA, UnB; MARIA DE LOURDES MENDES DE SOUZA, CTAA; OTNIEL FREITAS SILVA, CTAA; JUREMA DO SOCORRO AZEVEDO DIAS, CPAF-AP; LUIS I. B. KANZAKI, UnB; ROGERIO E. HANADA, INPA; RENATA M. L. C. MESQUITA, INPA; RIVADALVE COELHO GONCALVES, CPAF-AC; VIRGINIA DE SOUZA ALVARES, CPAF-AC; DANIELA MATIAS DE C BITTENCOURT, CPAA; ROBERTO N. G. MILLER, UnB. |
Título: |
Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
BMC Microbiology, v. 14, n. 138, 2014. |
DOI: |
10.1186/1471-2180-14-138 |
Idioma: |
Inglês |
Conteúdo: |
Background: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species. MenosBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius ... Mostrar Tudo |
Palavras-Chave: |
Castanha-do-brasil; Pragas e doenças. |
Thesagro: |
Aflatoxina. |
Thesaurus Nal: |
Aspergillus. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102994/1/castanha-Robert.pdf
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Marc: |
LEADER 02820naa a2200301 a 4500 001 2008479 005 2016-02-22 008 2014 bl --- 0-- u #d 024 7 $a10.1186/1471-2180-14-138$2DOI 100 1 $aMIDORIKAWA, G. E. O. 245 $aCharacterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level.$h[electronic resource] 260 $c2014 520 $aBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species. 650 $aAspergillus 650 $aAflatoxina 653 $aCastanha-do-brasil 653 $aPragas e doenças 700 1 $aSOUZA, M. de L. M. de 700 1 $aSILVA, O. F. 700 1 $aDIAS, J. do S. A. 700 1 $aKANZAKI, L. I. B. 700 1 $aHANADA, R. E. 700 1 $aMESQUITA, R. M. L. C. 700 1 $aGONCALVES, R. C. 700 1 $aALVARES, V. S. 700 1 $aBITTENCOURT, D. M. C. 700 1 $aMILLER, R. N. G. 773 $tBMC Microbiology$gv. 14, n. 138, 2014.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Registros recuperados : 44 | |
9. | | PAIM, G. A.; LIMA, Y. S.; SILVA, E. M. S.; CORREIA, R. C. Levantamento socioeconômico e perfil dos apicultores do município de Remanso, BA. In: CONGRESSO BRASILEIRO DE APICULTURA, 22.; CONGRESSO BRASILEIRO DE MELIPONICULTURA, 7., 2018, Joinville, SC. Polinização, tecnologia oportunidades e desafio para o criador de abelha no Brasil: anais. Joinville, SC: CBA, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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11. | | NEVES, R. A. D; LIMA, H. E. de; SILVA, E. E. da; QUEIROZ, E. S.; LIMA, Y. P. Composto orgânico produzido com resíduos da poda fitossanitária de cupuaçuzeiros infestados por vassoura-de-bruxa para produção de mudas de hortaliças. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 25., 2017; REUNIÃO ANUAL DA SOCIEDADE INTERAMERICANA DE HORTICULTURA TROPICAL, 62., 2017. Resumos... Porto Seguro, BA: SIHT, 2017.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Roraima. |
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14. | | MARQUES, J. B. B.; FERREIRA, J. L.; CASTAGNINO, J. O. G.; MARQUES, M. B.; LIMA, Y. R. Economic result of rice crop in the 2019 grow season. In: INTERNATIONAL TEMPERATE RICE CONFERENCE, 7., 2020, Pelotas. Science & Innovation: feeding a world of 10 billion people: proceedings. Brasília, DF: Embrapa, 2020. Não paginado.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sul. |
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17. | | SANTOS, K. P. dos; LIMA, Y. S.; BONFIM, B. R. de S.; MARINHO, A. M. C.; RIBEIRO, M. de F. Nicho trófico de Abelha-branca (Frieseomelitta doederelini) na região do Submédio do Vale do São Francisco. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 12., 2017, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2017. p. 219-225. (Embrapa Semiárido. Documentos, 279).Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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18. | | OLIVEIRA, P. de A.; ALÉCIO, M. R.; PIMENTEL, F. A.; LIMA, Y. C.; FAZOLIN, M.; VEIGA JÚNIOR, V. F. Quantificação dos constituintes majoritários de extratos de timbó (Derris ssp e Lonchocarpus ssp). In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 34., 2011, Florianópolis. Química para um mundo melhor. Florianópolis: SBQ, 2011. 1 p.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Acre. |
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19. | | ARZABE, C.; SKUK, G.; SANTANA, G. G.; DELFIM, F. R.; LIMA, Y. C. C. de; ABRANTES, S. H. F. Herpetofauna da área de Curimataú, Paraíba. In: ARAÚJO, F. S. de; RODAL, M. J. N.; BARBOSA, M. R. de V. (Org.). Análise das variações da biodiversidade do bioma caatinga: suporte a estratégias regionais de conservação. Brasília, DF: Ministério do Meio Ambiente, Secretaria de Biodiversidade e Florestas, 2005. p.259-273. (Biodiversidade, 12).Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Meio-Norte. |
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20. | | NEVES, R. A. D.; LIMA, H. E. de; SILVA, E. E. da; QUEIROZ, E. S.; LIMA, Y. P. de. Utilização de composto orgânico produzido a partir de resíduos da poda fitossanitária de cupuaçuzeiros infectados por Moniliophthora Perniciosa e de diferentes fontes de nitrogênio como substrato para produção de mudas de hortaliças. In: GOMIDE, P. H. O.; FALCÃO, M. T. (Org.). Sociobiodiversidade Amazônica: saberes, olhares e práticas agroecológicas. 1. ed. Boa Vista, RR : UERR Edições, 2021.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Roraima. |
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Registros recuperados : 44 | |
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