Data/hora: 05/06/2024 04:46:40

Biblioteca(s): | Embrapa Agroindústria de Alimentos; Embrapa Agroindústria Tropical.
Data corrente: | 01/10/2020
Data da última atualização: | 02/10/2020
Autoria: | SANTOS, K. P. dos; SILVA, C. M.; BRIGIDA, A. I. S.; GONÇALVES, L. R. B.
Afiliação: | Kimberle Paiva dos Santos, Universidade Federal do Ceará; CAROLINE MELLINGER SILVA, CTAA; ANA IRAIDY SANTA BRIGIDA, CNPAT; Luciana Rocha Barros Gonçalves, Universidade Federal do Ceará.
Título: | Modifying alcalase activity and stability by immobilization onto chitosan aiming at the production of bioactive peptides by hydrolysis of tilapia skin gelatin.
Ano de publicação: | 2020
Fonte/Imprenta: | Process biochemistry, v. 97, p. 27-36, 2020.
DOI: | https://doi.org/10.1016/j.procbio.2020.06.019
Idioma: | Inglês
Conteúdo: | The protease from Bacillus licheniformis, commercially known as Alcalase (R), was insolubilized and stabilized by immobilization onto activated chitosan. Activation with different agents, such as glutaraldehyde (GLU-Chi), glyoxyl (GLY-Chi) and divinyl sulfone (DVS-Chi) was investigated. The effect of the immobilization protocol, for instance different pH and times, were also evaluated. GLU-Chi showed the highest activity (35.6UNPA/g) with the smallest substrate (N-Boc-L-alanine p-nitrophenyl-ester, NPA), while GLY-Chi showed the highest activity (1.5 UAzocasein/g) using the greatest substrate (azocasein). A 24-h immobilization period was enough to stabilize the enzyme using the three supports under almost all conditions. Operational stability in azocasein hydrolysis was assayed and GLU-Chi showed no activity loss during 5 cycles. DVS-Chi retained around 70 % of its initial activity after the fifth cycle, whereas GLY-Chi activity retained only 10 %. Finally, the biocatalysts were used in the hydrolysis of tilapia skin gelatin aiming the production of peptides with antioxidant activity. The protein hy-drolysates obtained using GLU-Chi presented the highest antioxidant activity (36.7 mu M Trolox Eq). However, the best results of operational stability were obtained using DVS-Chi, which did not lose its initial activity after 3 consecutive cycles of gelatin hydrolysis.
Palavras-Chave: | Alcalase; Immobilization; Skin gelatin; Stability.
Thesagro: | Tilápia.
Thesaurus Nal: | Chitosan; Food technology; Protein hydrolysates.
Categoria do assunto: | --
Registro original: | Embrapa Agroindústria de Alimentos (CTAA)

Exemplares:

CTAA - (15026-1) - UPC - AP - DD -  -  -  - DIS
CNPAT - (16501-1) - UPC - AP - DD -  -  - ART20078 - DIS