Data/hora: 10/06/2024 05:30:24

Biblioteca(s): 	Embrapa Unidades Centrais.	
Data corrente: 	15/02/2016	
Data da última atualização: 	29/12/2017	
Autoria: 	CAMPOS, A. P.; MIRANDA, D. F. H.; ALVES, G. B. B.; CARNEIRO, M. S.; PRIANTI, M. G.; GONÇALVES, L. M. F.; CASTRO, V.; COSTA, F. A. L.	
Afiliação: 	ÂNGELA P. CAMPOS, UFPI; DAYANE F. H. MIRANDA, UFPI; GEÓRGIA B. B. ALVES, UFPI; MICHERLENE S. CARNEIRO, UFPI; MARIA G. PRIANTI, UFPI; LARISSA M. F. GONÇALVES, UFPI; VANESSA CASTRO, Instituo Biológico de São Paulo; FRANCISCO A. L. COSTA, UFPI.	
Título: 	The role of alveolar type II cells in swine leptospirosis.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 35, n. 7, p. 620-626. jul. 2015.	
Idioma: 	Inglês	
Notas: 	Título em português: O papel das células alveolares tipo II na leptospirose suína.	
Conteúdo: 	This study aimed to investigate a possible relationship between alveolar type II cells and the inflammatory response to infection with Leptospira spp., and thus comprise a further element that can be involved in the pathogenesis of lung injury in naturally infected pigs. The study group consisted of 73 adult pigs that were extensively reared and slaughtered in Teresina, Piauí state, and Timon, Maranhão state, Brazil. The diagnosis of leptospirosis was made using the microscopic agglutination test (MAT) aided by immunohistochemistry and polymerase chain reaction. The MAT registered the occurrence of anti-Leptospira antibodies in 10.96% (8/73) of the pigs. Immunohistochemistry allowed for the visualization of the Leptospira spp. antigen in the lungs of 87.67% (64/73) of the pigs. There was hyperplasia of bronchus-associated lymphoid tissue and circulatory changes, such as congestion of alveolar septa, parenchymal hemorrhage and edema within the alveoli. Lung inflammation was more intense (p = 0.0312) in infected animals, which also showed increased thickening of the alveolar septa (p = 0.0006). Evaluation of alveolar type II (ATII) cells using an anti-TTF-1 (Thyroid Transcription Factor-1) antibody showed that there were more immunostained cells in the non-infected pigs (53.8%) than in the infected animals (46.2%) and that there was an inverse correlation between TTF-1 positive cells and the inflammatory infiltrate. There was no amplification of Leptospira DNA in the lung samples, but leptospiral DNA amplification was observed in the kidneys. The results of this study showed that a relationship exists between a decrease in alveolar type II cells and a leptospire infection. Thus, this work points to the importance of studying the ATII cells as a potential marker of the level of lung innate immune response during leptospirosis in pigs.	
Thesaurus Nal: 	Immunohistochemistry; Lungs; Swine.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/138963/1/The-role-of-alveolar-type.pdf	
Registro original: 	Embrapa Unidades Centrais (AI-SEDE)	
Exemplares: 	AI-SEDE - (58778-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS