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Registro Completo |
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
16/03/2006 |
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Data da última atualização: |
16/11/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
WADT, L. H. de O.; KAINER, K. A.; GOMES-SILVA, D. A. P. |
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Afiliação: |
LUCIA HELENA DE OLIVEIRA WADT, CPAF-AC; KAREN A. KAINER, University of Florida; DAISY A. P. GOMES SILVA, Universidade Federal do Acre. |
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Título: |
Characterization of adult Brazil nut trees in Extractive Reserve Chico Mendes, Acre, Brazil. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
In: WORKING FOREST IN THE TROPICS, 2002, Gainesville. Conservation through sustainable management: abstract book and program. Gainesville: University of Florida, 2002. |
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Páginas: |
p. 110. |
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Idioma: |
Inglês |
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Conteúdo: |
Brazil nut (Bertholletia excelsa) is an emergent canopy species occurring on non-flooded lands (terra firme) in the Amazon basin. Its seeds or nuts, which are collected in the wild and sold on the international market, are an important income source for extractive families living throughout the species range. Seed collection appears to be ecologically sustainable as recent comparative studies demonstrate no negative impact on population structure. Still, technologies for improving production are needed to enhance the economic sustainability of extractive reserves. The objective of this preliminary study, executed in a 465-ha Brazil nut-rich forest within Extractive Reserve Chico Mendes, was to examine factors affecting Brazil nut productivity, focusing on effects of vine loads on adults. Only trees >- 30 cm DBH were considered adults and included in the analysis given that no tree below this cutoff produced fruit. For each tree, DBH was measured, and GPS coordinates and categorical data on vine load, nut production, and crown form and position were collected. Densities and diameter distributions were calculated for the stand. |
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Palavras-Chave: |
Acre; Amazonia Occidental; Amazônia Ocidental; Áreas de conservación; Castanha do brasil; Nuez del Brasil; Rendimiento de los cultivos; RESEX Chico Mendes; Western Amazon. |
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Thesagro: |
Bertholletia Excelsa; Castanha do Para; Produtividade. |
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Thesaurus Nal: |
Brazil nuts; Conservation areas; Crop yield. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140204/1/12787.pdf
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Marc: |
LEADER 02225nam a2200325 a 4500
001 1503685
005 2023-11-16
008 2002 bl uuuu u00u1 u #d
100 1 $aWADT, L. H. de O.
245 $aCharacterization of adult Brazil nut trees in Extractive Reserve Chico Mendes, Acre, Brazil.$h[electronic resource]
260 $aIn: WORKING FOREST IN THE TROPICS, 2002, Gainesville. Conservation through sustainable management: abstract book and program. Gainesville: University of Florida$c2002
300 $ap. 110.
520 $aBrazil nut (Bertholletia excelsa) is an emergent canopy species occurring on non-flooded lands (terra firme) in the Amazon basin. Its seeds or nuts, which are collected in the wild and sold on the international market, are an important income source for extractive families living throughout the species range. Seed collection appears to be ecologically sustainable as recent comparative studies demonstrate no negative impact on population structure. Still, technologies for improving production are needed to enhance the economic sustainability of extractive reserves. The objective of this preliminary study, executed in a 465-ha Brazil nut-rich forest within Extractive Reserve Chico Mendes, was to examine factors affecting Brazil nut productivity, focusing on effects of vine loads on adults. Only trees >- 30 cm DBH were considered adults and included in the analysis given that no tree below this cutoff produced fruit. For each tree, DBH was measured, and GPS coordinates and categorical data on vine load, nut production, and crown form and position were collected. Densities and diameter distributions were calculated for the stand.
650 $aBrazil nuts
650 $aConservation areas
650 $aCrop yield
650 $aBertholletia Excelsa
650 $aCastanha do Para
650 $aProdutividade
653 $aAcre
653 $aAmazonia Occidental
653 $aAmazônia Ocidental
653 $aÁreas de conservación
653 $aCastanha do brasil
653 $aNuez del Brasil
653 $aRendimiento de los cultivos
653 $aRESEX Chico Mendes
653 $aWestern Amazon
700 1 $aKAINER, K. A.
700 1 $aGOMES-SILVA, D. A. P.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/12/2017 |
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Data da última atualização: |
03/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. |
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Afiliação: |
VALÉRIA POLESE, UFRRJ; LEITON DE PAULA SOARES, UFRRJ; PAULA RENATA ALVES DA SILVA, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
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Título: |
Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017. |
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ISSN: |
1572-9699 |
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DOI: |
https://doi.org/10.1007/s10482-017-0906-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Quantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism. MenosQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Toget... Mostrar Tudo |
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Palavras-Chave: |
Internal normalizing; Organic acids; Relative expression; Sugar transport. |
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Thesaurus NAL: |
nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02682naa a2200265 a 4500
001 2083708
005 2018-01-03
008 2017 bl uuuu u00u1 u #d
022 $a1572-9699
024 7 $ahttps://doi.org/10.1007/s10482-017-0906-7$2DOI
100 1 $aPOLESE, V.
245 $aSelection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103.$h[electronic resource]
260 $c2017
520 $aQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism.
650 $anitrogen fixation
653 $aInternal normalizing
653 $aOrganic acids
653 $aRelative expression
653 $aSugar transport
700 1 $aSOARES, C. de P.
700 1 $aSILVA, P. R. A. da
700 1 $aARAUJO, J. L. S. de
700 1 $aBALDANI, J. I.
700 1 $aVIDAL, M. S.
773 $tAntonie van Leeuwenhoekv, 110$gn. 12, p. 1555-1568, 2017.
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