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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/10/2021 |
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Data da última atualização: |
03/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COTTA, S. P. M.; MARINS, M. S.; MARRIEL, I. E.; LANA, U. G. de P.; GOMES, E. A.; FIGUEIREDO, J. E. F.; OLIVEIRA-PAIVA, C. A. |
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Afiliação: |
Centro Universitário de Sete Lagoas, UNIFEMM; Universidade Federal de Lavras; IVANILDO EVODIO MARRIEL, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; ELIANE APARECIDA GOMES, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS. |
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Título: |
Thermo-resistant enzyme-producing microorganisms isolated from composting. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Brazilian Journal of Biology, v. 83, e244205, 2023. |
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Idioma: |
Inglês |
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Notas: |
Publicado em: 27 mar. 2021. |
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Conteúdo: |
Organo-mineral fertilizers supplemented with biological additives are an alternative to chemical fertilizers. In this study, thermoresistant microorganisms from composting mass were isolated by two-step procedures. First, samples taken at different time points and temperatures (33 days at 52 ºC, 60 days at 63 ºC, and over 365 days at 26 ºC) were pre-incubated at 80o C for 30 minutes. Second, the microbial selection by in vitro culture-based methods and heat shock at 60o C and 100o C for 2h and 4h. Forty-one isolates were able to grow at 60 °C for 4h; twenty-seven at 100 °C for 2h, and two at 100 °C for 4h. The molecular identification by partial sequencing of the 16S ribosomal gene using universal primers revealed that thirty-five isolates were from eight Bacillus species, one Brevibacillus borstelensis, three Streptomyces thermogriseus, and two fungi (Thermomyces lanuginosus and T. dupontii). Data from amylase, phytase, and cellulase activity assays and the enzymatic index (EI) showed that 38 of 41 thermo-resistant isolates produce at least one enzyme. For amylase activity, the highest EI value was observed in Bacillus licheniformis (isolate 21C2, EI= 4.11), followed by Brevibacillus borstelensis (isolate 6C2, EI= 3.66), Bacillus cereus (isolate 18C2, EI= 3.52), and Bacillus paralicheniformis (isolate 20C2, EI= 3.34). For phytase, the highest EI values were observed for Bacillus cereus (isolate 18C2, EI= 2.30) and Bacillus licheniformis (isolate 3C1, EI= 2.15). Concerning cellulose production, B. altitudinis (isolate 6C1) was the most efficient (EI= 6.40), followed by three Bacillus subtilis (isolates 9C1, 16C2, and 19C2) with EI values of 5.66, 5.84, and 5.88, respectively, and one B. pumilus (isolate 27C2, EI= 5.78). The selected microorganisms are potentially useful as a biological additive in organo-mineral fertilizers and other biotechnological processes. MenosOrgano-mineral fertilizers supplemented with biological additives are an alternative to chemical fertilizers. In this study, thermoresistant microorganisms from composting mass were isolated by two-step procedures. First, samples taken at different time points and temperatures (33 days at 52 ºC, 60 days at 63 ºC, and over 365 days at 26 ºC) were pre-incubated at 80o C for 30 minutes. Second, the microbial selection by in vitro culture-based methods and heat shock at 60o C and 100o C for 2h and 4h. Forty-one isolates were able to grow at 60 °C for 4h; twenty-seven at 100 °C for 2h, and two at 100 °C for 4h. The molecular identification by partial sequencing of the 16S ribosomal gene using universal primers revealed that thirty-five isolates were from eight Bacillus species, one Brevibacillus borstelensis, three Streptomyces thermogriseus, and two fungi (Thermomyces lanuginosus and T. dupontii). Data from amylase, phytase, and cellulase activity assays and the enzymatic index (EI) showed that 38 of 41 thermo-resistant isolates produce at least one enzyme. For amylase activity, the highest EI value was observed in Bacillus licheniformis (isolate 21C2, EI= 4.11), followed by Brevibacillus borstelensis (isolate 6C2, EI= 3.66), Bacillus cereus (isolate 18C2, EI= 3.52), and Bacillus paralicheniformis (isolate 20C2, EI= 3.34). For phytase, the highest EI values were observed for Bacillus cereus (isolate 18C2, EI= 2.30) and Bacillus licheniformis (isolate 3C1, EI= 2.15). Concerning c... Mostrar Tudo |
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Palavras-Chave: |
Microrganismo termoresistente. |
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Thesagro: |
Enzima; Fertilizante; Microrganismo. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227293/1/Thermo-resistant.pdf
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Marc: |
LEADER 02644naa a2200253 a 4500
001 2135666
005 2022-02-03
008 2023 bl uuuu u00u1 u #d
100 1 $aCOTTA, S. P. M.
245 $aThermo-resistant enzyme-producing microorganisms isolated from composting.$h[electronic resource]
260 $c2023
500 $aPublicado em: 27 mar. 2021.
520 $aOrgano-mineral fertilizers supplemented with biological additives are an alternative to chemical fertilizers. In this study, thermoresistant microorganisms from composting mass were isolated by two-step procedures. First, samples taken at different time points and temperatures (33 days at 52 ºC, 60 days at 63 ºC, and over 365 days at 26 ºC) were pre-incubated at 80o C for 30 minutes. Second, the microbial selection by in vitro culture-based methods and heat shock at 60o C and 100o C for 2h and 4h. Forty-one isolates were able to grow at 60 °C for 4h; twenty-seven at 100 °C for 2h, and two at 100 °C for 4h. The molecular identification by partial sequencing of the 16S ribosomal gene using universal primers revealed that thirty-five isolates were from eight Bacillus species, one Brevibacillus borstelensis, three Streptomyces thermogriseus, and two fungi (Thermomyces lanuginosus and T. dupontii). Data from amylase, phytase, and cellulase activity assays and the enzymatic index (EI) showed that 38 of 41 thermo-resistant isolates produce at least one enzyme. For amylase activity, the highest EI value was observed in Bacillus licheniformis (isolate 21C2, EI= 4.11), followed by Brevibacillus borstelensis (isolate 6C2, EI= 3.66), Bacillus cereus (isolate 18C2, EI= 3.52), and Bacillus paralicheniformis (isolate 20C2, EI= 3.34). For phytase, the highest EI values were observed for Bacillus cereus (isolate 18C2, EI= 2.30) and Bacillus licheniformis (isolate 3C1, EI= 2.15). Concerning cellulose production, B. altitudinis (isolate 6C1) was the most efficient (EI= 6.40), followed by three Bacillus subtilis (isolates 9C1, 16C2, and 19C2) with EI values of 5.66, 5.84, and 5.88, respectively, and one B. pumilus (isolate 27C2, EI= 5.78). The selected microorganisms are potentially useful as a biological additive in organo-mineral fertilizers and other biotechnological processes.
650 $aEnzima
650 $aFertilizante
650 $aMicrorganismo
653 $aMicrorganismo termoresistente
700 1 $aMARINS, M. S.
700 1 $aMARRIEL, I. E.
700 1 $aLANA, U. G. de P.
700 1 $aGOMES, E. A.
700 1 $aFIGUEIREDO, J. E. F.
700 1 $aOLIVEIRA-PAIVA, C. A.
773 $tBrazilian Journal of Biology$gv. 83, e244205, 2023.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
22/02/2022 |
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Data da última atualização: |
23/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
SILVA, A. C. A. da; TRINDADE, R. dos S.; MARIZ, B. L.; RIBEIRO, M. R.; NETTO, D. A. M.; PARELLA, N. N. L. D.; TRINDADE, B. M. C.; SOUZA, I. R. P. de; GUIMARAES, L. J. M.; GUIMARAES, P. E. de O. |
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Afiliação: |
ANA CAROLINA APARECIDA DA SILVA; ROBERTO DOS SANTOS TRINDADE, CNPMS; BRUNA LOPES MARIZ, Universidade Federal de São João Del-Rei; MARIANA RODRIGUES RIBEIRO, Universidade Federal de Viçosa; DEA ALÉCIA MARTINS NETTO, Pesquisadora aposentada da Embrapa Milho e Sorgo; NÁDIA NARDELY LACERDA DURÃES PARELLA, Universidade Federal de São João Del-Rei; BIANCA MACHADO CAMPO TRINDADE, Universidade Federal Rural do Rio de Janeiro; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; LAURO JOSE MOREIRA GUIMARAES, CNPMS; PAULO EVARISTO DE O GUIMARAES, CNPMS. |
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Título: |
Physiological quality in seeds from haploid inducers in maize. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, v. 20, e1214, 2021. |
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DOI: |
https://doi.org/10.18512/rbms2021v20e1214 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of the present work was to evaluate the physiological quality in seeds of haploidy inducers of different types and origins. To this end, between December 2015 and March 2016, a series of physiological quality assessments were conducted on nine genotypes inducing haploidy in corn, namely: the gymnogenetic inducers Stock 6, TAIL P1 and TAIL P2; the hybrid between the TAIL P1 x TAIL P2 inductors; the hybrid TAIL P2 x TAIL P1, analogous to the previous hybrid, but with inversion of the female parent at the crossing; and androgenetic inducers W23, 90109 igig, 91202 igig and 91207 igig. The tests carried out were germination, accelerated aging, emergence in a bed, emergence index, fresh and dry mass, length of aerial part and root and the ratio between these characteristics, the humidity and the weight of 100 seeds. The results indicated that the physiological quality was improved throughout the selection for greater haploid induction. The haploidy induction system (andorgenetic or gymnogenetic) does not interfere with physiological quality, however the results obtained reinforce the need for care in the multiplication and conservation of haploidy inducing seeds in tropical conditions for their use in breeding. |
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Palavras-Chave: |
Germintion and vigor; Haploids induction; Indução de haploidia; Tropicalização; Tropicalization. |
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Thesagro: |
Germinação; Milho; Vigor Hibrido; Zea Mays. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/231672/1/Physiological-quality.pdf
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Marc: |
LEADER 02263naa a2200349 a 4500
001 2140299
005 2022-02-23
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.18512/rbms2021v20e1214$2DOI
100 1 $aSILVA, A. C. A. da
245 $aPhysiological quality in seeds from haploid inducers in maize.$h[electronic resource]
260 $c2021
520 $aThe objective of the present work was to evaluate the physiological quality in seeds of haploidy inducers of different types and origins. To this end, between December 2015 and March 2016, a series of physiological quality assessments were conducted on nine genotypes inducing haploidy in corn, namely: the gymnogenetic inducers Stock 6, TAIL P1 and TAIL P2; the hybrid between the TAIL P1 x TAIL P2 inductors; the hybrid TAIL P2 x TAIL P1, analogous to the previous hybrid, but with inversion of the female parent at the crossing; and androgenetic inducers W23, 90109 igig, 91202 igig and 91207 igig. The tests carried out were germination, accelerated aging, emergence in a bed, emergence index, fresh and dry mass, length of aerial part and root and the ratio between these characteristics, the humidity and the weight of 100 seeds. The results indicated that the physiological quality was improved throughout the selection for greater haploid induction. The haploidy induction system (andorgenetic or gymnogenetic) does not interfere with physiological quality, however the results obtained reinforce the need for care in the multiplication and conservation of haploidy inducing seeds in tropical conditions for their use in breeding.
650 $aGerminação
650 $aMilho
650 $aVigor Hibrido
650 $aZea Mays
653 $aGermintion and vigor
653 $aHaploids induction
653 $aIndução de haploidia
653 $aTropicalização
653 $aTropicalization
700 1 $aTRINDADE, R. dos S.
700 1 $aMARIZ, B. L.
700 1 $aRIBEIRO, M. R.
700 1 $aNETTO, D. A. M.
700 1 $aPARELLA, N. N. L. D.
700 1 $aTRINDADE, B. M. C.
700 1 $aSOUZA, I. R. P. de
700 1 $aGUIMARAES, L. J. M.
700 1 $aGUIMARAES, P. E. de O.
773 $tRevista Brasileira de Milho e Sorgo$gv. 20, e1214, 2021.
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