|
|
Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
01/03/2016 |
Data da última atualização: |
19/06/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; ESPUNY, M. del R.; RODRÍGUEZ-CARVAJAL, M. A.; SORIA-DÍAZ, M. E.; NAKATANI, A. S.; HUNGRIA, M.; JAVIER OLLERO, F.; MEGÍAS, M. |
Afiliação: |
PABLO DEL CERRO, Universidad de Sevilla; AMANDA ALVES PAIVA ROLLA-SANTOS; DOUGLAS FABIANO GOMES; BETTINA BERQUÓ MARKS; MARÍA DEL ROSARIO ESPUNY, Universidad de Sevilla; MIGUEL ÁNGEL RODRÍGUEZ-CARVAJAL, Universidad de Sevilla; MARÍA EUGENIA SORIA-DÍAZ, CITIUS; ANDRÉ SHIGUEYOSHI NAKATAN; MARIANGELA HUNGRIA DA CUNHA, CNPSO; FRANCISCO JAVIER OLLERO; MANUEL MEGÍAS. |
Título: |
Opening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
BMC Genomics, v. 16, n. 1, p. 864, Oct. 2015. |
ISSN: |
1471-2164 |
DOI: |
10.1186/s12864-015-2033-z |
Idioma: |
Inglês |
Conteúdo: |
Transcription of nodulation genes in rhizobial species is orchestrated by the regulatory nodD gene. Rhizobium tropici strain CIAT 899 is an intriguing species in possessing features such as broad host range, high tolerance of abiotic stresses and, especially, by carrying the highest known number of nodD genes?five?and the greatest diversity of Nod factors (lipochitooligosaccharides, LCOs). Here we shed light on the roles of the multiple nodD genes of CIAT 899 by reporting, for the first time, results obtained with nodD3, nodD4 and nodD5 mutants. The three nodD mutants were built by insertion of ? interposon. Nod factors were purified and identified by LC-MS/MS analyses. In addition, nodD1 and nodC relative gene expressions were measured by quantitative RT-PCR in the wt and derivative mutant strains. Phenotypic traits such as exopolysaccharide (EPS), lipopolysaccharide (LPS), swimming and swarming motilities, biofilm formation and indole acetid acid (IAA) production were also perfomed. All these experiments were carried out in presence of both inducers of CIAT 899, apigenin and salt. Finally, nodulation assays were evaluated in up to six different legumes, including common bean (Phaseolus vulgaris L.). Phenotypic and symbiotic properties, Nod factors and gene expression of nodD3, nodD4 and nodD5 mutants were compared with those of the wild-type (WT) CIAT 899, both in the presence and in the absence of the nod-geneinducing molecule apigenin and of saline stress. No differences between the mutants and the WT were observed in exopolysaccharide (EPS) and lipopolysaccharide (LPS) profiles, motility, indole acetic acid (IAA) synthesis or biofilm production, either in the presence, or in the absence of inducers. Nodulation studies demonstrated the most complex regulatory system described so far, requiring from one (Leucaena leucocephala, Lotus burtii) to four (Lotus japonicus) nodD genes. Up to 38 different structures of Nod factors were detected, being higher under salt stress, except for the nodD5 mutant; in addition, a high number of structures was synthesized by the nodD4 mutant in the absence of any inducer. Probable activator (nodD3 and nodD5) or repressor roles (nodD4), possibly via nodD1 and/or nodD2, were attributed to the three nodD genes. Expression of nodC, nodD1 and each nodD studied by RT-qPCR confirmed that nodD3 is an activator of nodD1, both in the presence of apigenin and salt stress. In contrast, nodD4 might be an inducer with apigenin and a repressor under saline stress, whereas nodD5 was an inducer under both conditions. MenosTranscription of nodulation genes in rhizobial species is orchestrated by the regulatory nodD gene. Rhizobium tropici strain CIAT 899 is an intriguing species in possessing features such as broad host range, high tolerance of abiotic stresses and, especially, by carrying the highest known number of nodD genes?five?and the greatest diversity of Nod factors (lipochitooligosaccharides, LCOs). Here we shed light on the roles of the multiple nodD genes of CIAT 899 by reporting, for the first time, results obtained with nodD3, nodD4 and nodD5 mutants. The three nodD mutants were built by insertion of ? interposon. Nod factors were purified and identified by LC-MS/MS analyses. In addition, nodD1 and nodC relative gene expressions were measured by quantitative RT-PCR in the wt and derivative mutant strains. Phenotypic traits such as exopolysaccharide (EPS), lipopolysaccharide (LPS), swimming and swarming motilities, biofilm formation and indole acetid acid (IAA) production were also perfomed. All these experiments were carried out in presence of both inducers of CIAT 899, apigenin and salt. Finally, nodulation assays were evaluated in up to six different legumes, including common bean (Phaseolus vulgaris L.). Phenotypic and symbiotic properties, Nod factors and gene expression of nodD3, nodD4 and nodD5 mutants were compared with those of the wild-type (WT) CIAT 899, both in the presence and in the absence of the nod-geneinducing molecule apigenin and of saline stress. No differences... Mostrar Tudo |
Thesagro: |
Bacteriologia do solo; Fixação de nitrogênio; Nodulação; Rhizobium. |
Thesaurus Nal: |
Bacteriology; Nitrogen fixation; Nodulation. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140562/1/12864-2015-Article-2033.pdf
|
Marc: |
LEADER 03591naa a2200349 a 4500 001 2039242 005 2017-06-19 008 2015 bl uuuu u00u1 u #d 022 $a1471-2164 024 7 $a10.1186/s12864-015-2033-z$2DOI 100 1 $aCERRO, P. del 245 $aOpening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899.$h[electronic resource] 260 $c2015 520 $aTranscription of nodulation genes in rhizobial species is orchestrated by the regulatory nodD gene. Rhizobium tropici strain CIAT 899 is an intriguing species in possessing features such as broad host range, high tolerance of abiotic stresses and, especially, by carrying the highest known number of nodD genes?five?and the greatest diversity of Nod factors (lipochitooligosaccharides, LCOs). Here we shed light on the roles of the multiple nodD genes of CIAT 899 by reporting, for the first time, results obtained with nodD3, nodD4 and nodD5 mutants. The three nodD mutants were built by insertion of ? interposon. Nod factors were purified and identified by LC-MS/MS analyses. In addition, nodD1 and nodC relative gene expressions were measured by quantitative RT-PCR in the wt and derivative mutant strains. Phenotypic traits such as exopolysaccharide (EPS), lipopolysaccharide (LPS), swimming and swarming motilities, biofilm formation and indole acetid acid (IAA) production were also perfomed. All these experiments were carried out in presence of both inducers of CIAT 899, apigenin and salt. Finally, nodulation assays were evaluated in up to six different legumes, including common bean (Phaseolus vulgaris L.). Phenotypic and symbiotic properties, Nod factors and gene expression of nodD3, nodD4 and nodD5 mutants were compared with those of the wild-type (WT) CIAT 899, both in the presence and in the absence of the nod-geneinducing molecule apigenin and of saline stress. No differences between the mutants and the WT were observed in exopolysaccharide (EPS) and lipopolysaccharide (LPS) profiles, motility, indole acetic acid (IAA) synthesis or biofilm production, either in the presence, or in the absence of inducers. Nodulation studies demonstrated the most complex regulatory system described so far, requiring from one (Leucaena leucocephala, Lotus burtii) to four (Lotus japonicus) nodD genes. Up to 38 different structures of Nod factors were detected, being higher under salt stress, except for the nodD5 mutant; in addition, a high number of structures was synthesized by the nodD4 mutant in the absence of any inducer. Probable activator (nodD3 and nodD5) or repressor roles (nodD4), possibly via nodD1 and/or nodD2, were attributed to the three nodD genes. Expression of nodC, nodD1 and each nodD studied by RT-qPCR confirmed that nodD3 is an activator of nodD1, both in the presence of apigenin and salt stress. In contrast, nodD4 might be an inducer with apigenin and a repressor under saline stress, whereas nodD5 was an inducer under both conditions. 650 $aBacteriology 650 $aNitrogen fixation 650 $aNodulation 650 $aBacteriologia do solo 650 $aFixação de nitrogênio 650 $aNodulação 650 $aRhizobium 700 1 $aROLLA-SANTOS, A. A. P. 700 1 $aGOMES, D. F. 700 1 $aMARKS, B. B. 700 1 $aESPUNY, M. del R. 700 1 $aRODRÍGUEZ-CARVAJAL, M. A. 700 1 $aSORIA-DÍAZ, M. E. 700 1 $aNAKATANI, A. S. 700 1 $aHUNGRIA, M. 700 1 $aJAVIER OLLERO, F. 700 1 $aMEGÍAS, M. 773 $tBMC Genomics$gv. 16, n. 1, p. 864, Oct. 2015.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 44 | |
2. | | GOMES, D. F.; BATISTA, J. S. S.; HUNGRIA, M. Two-dimensional proteomic reference map of Bradyrhizobium diazoefficiens strain CPAC 7 (=SEMIA 5080). In: IBEROAMERICAN CONFERENCE ON BENEFICIAL PLANT - MICROORGANISM - ENVIRONMENT INTERACTIONS, 2.; NATIONAL MEETING OF THE SPANISH SOCIETY OF NITROGEN FIXATION, 14.; LATIN AMERICAN MEETING ON RHIZOBIOLOGY, 26.; SPANISH-PROTUGUESE CONGRESS ON NITROGEN FIXATION, 3., 2013, Sevilla. Microorganisms for future agriculture. Sevilla: Universidad de Sevilla; ALAR; SEFIN, 2013. p. 141-142.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
3. | | RIBEIRO, R. A.; GOMES, D. F.; DELAMUTA, J. R. M.; NOGUEIRA, M. A.; HUNGRIA, M. Análises genômicas na definição de novas espécies. In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA - RELAR, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. p. 130. Editores: Mariangela Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
4. | | SCHIAVON, A. L.; RODRIGUES, E. P.; BATISTA, J. S. S.; GOMES, D. F.; HUNGRIA, M. Análise proteômica de raízes de feijão (Phaseolus vulgaris) inoculadas com Rhizobium tropici. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 9-12. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
8. | | BATISTA, J. S. da S.; RODRIGUES, E. P.; TORRES, A. R.; GOMES, D. F.; HUNGRIA, M. Efeito da inoculação com Bradyrhizobium japonicum no proteoma de raízes de soja. In: CONGRESSO BRASILEIRO DE MICROBILOGIA, 26.; SIMPÓSIO INTERNACIONAL DE BACTÉRIAS LÁTICAS; ENCONTRO NACIONAL DE PROFESSORES DE MICROBIOLOGIA; SIMPÓSIO DE COLEÇÕES DE CULTURAS, 4., 2011, Foz do Iguaçu. Anais... São Paulo: SBM, 2011. Resumo, 776-1.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
9. | | GOMES, D. F.; CARDOSO, J. D.; MATOS, M. A.; ANDRADE, D. S.; HUNGRIA, M. Efeito da calagem e fosfatagem na diversidade de rhizobium-phaseolus em solo ácido. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25., 2009, Porto de Galinhas. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2009. Seção Resumos, ref. 2364-2. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
10. | | GOMES, D. F.; CARDOSO, J. C.; MATOS, M. A.; HUNGRIA, M.; ANDRADE, D. S. Effect of liming on plasmid profile of Rhizobium strains. In: INTERNATIONAL INCT SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 1., 2009, Curitiba. Program and index... Curitiba: INCT, 2009. Conference , P31.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
11. | | GOMES, D. F.; ROLLA-SANTOS, A. A. P.; BATISTA, J. S. S.; HUNGRIA, M. Relative expression of hypothetical protein-related genes for Bradyrhizobium diazoefficiens CPAC 7. In: IBEROAMERICAN CONFERENCE ON BENEFICIAL PLANT - MICROORGANISM - ENVIRONMENT INTERACTIONS, 2.; NATIONAL MEETING OF THE SPANISH SOCIETY OF NITROGEN FIXATION, 14.; LATIN AMERICAN MEETING ON RHIZOBIOLOGY, 26.; SPANISH-PROTUGUESE CONGRESS ON NITROGEN FIXATION, 3., 2013, Sevilla. Microorganisms for future agriculture. Sevilla: Universidad de Sevilla; ALAR; SEFIN, 2013. p. 145-146.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
14. | | GOMES, D. F.; BATISTA, J. S. da S.; SCHIAVON, A. L.; ANDRADE, D. S.; HUNGRIA, M. Proteomic profiling of Rhizobium tropici PRF 81: identification of conserved and specific responses to heat stress. BMC Microbiology, London, v. 12, n. 84, p. 1-12, May 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Soja. |
| |
18. | | TULLIO, L. D.; GOMES, D. F.; SILVA, L. P. da; HUNGRIA, M.; BATISTA, J. S. da S. Análise proteômica de rhizobium freirei PRF 81 sob estresse ácido. In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA - RELAR, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. p. 174. Editores: Mariangela Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
19. | | CARDOSO, J. D.; GOMES, D. F.; MATOS, M. A. de; ANDRADE, D. S.; CUNHA, M. H. da. Caracterização inter e intra-específica de estirpes elite de rizóbio. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25., 2009, Porto de Galinhas. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2009. Seção Resumos, ref. 2364-1. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
20. | | CARVALHO, F. M.; GOMES, D. F.; ORTIZ, M.; ALMEIDA, L. G.; SOUSA, R. C.; VASCONCELOS, A. T. R.; HUNGRIA, M. Genomic panorama, focused on the symbiotic context, of bradyrhizobium elkanii strains semia 587, semia 5019 and usda 76t (t é sobrescrito). In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA - RELAR, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. p. 178. Editores: Mariangela Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
| |
Registros recuperados : 44 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|