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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
02/08/2016 |
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Data da última atualização: |
28/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIOL, M. A.; GUERRERO, F. D.; OLIVEIRA, B. C. M. de; AQUINO, M. C. C. de; LOIOLA, S. H.; MELO, G. D. de; GOMES, A. H. de S.; KANAMURA, C. T.; GARCIA, M. V.; ANDREOTTI, R.; LIMA, V. M. F. de; BRESCIANI, K. D. S. |
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Afiliação: |
Milena Araúz Viol, UNESP; Felix D. Guerrero, UNESP; Bruno César Miranda de Oliveira, UNESP; Monally Conceição Costa de Aquino, UNESP; Saulo Hudson Loiola, UNESP; Guilherme Dias de Melo, UNESP; Aparecida Helena de Souza Gomes, Instituto Adolfo Lutz; Cristina Takami Kanamura, INSTITUTO ADOLFO LUTZ; Marcos Valério Garcia, FUNDECT; RENATO ANDREOTTI E SILVA, CNPGC; Valéria Marçal Félix de Lima, UNESP; Katia Denise Saraiva Bresciani, UNESP. |
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Título: |
Identification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Parasitology Research, v. 115, n, 9, p. 3479-3484, sept. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Sand flies are recognized as the major vector of canine visceral leishmaniasis. However, in some areas of Brazil where sand flies do not occur, this disease is found in humans and dogs. There has been speculation that ticks might play a role in transmission of canine visceral leishmaniasis and the DNA of Leishmania spp. has been reported in whole ticks. We investigated the presence of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus ticks collected from tickinfested dogs in two cities of Brazil. We used 66 dogs that tested positive and 33 that tested negative for Leishmania spp. according to direct cytological examination assays. Ten ticks were collected from each dog and dissected to collect the intestines, ovaries, and salivary glands for immunohistochemistry (IHC) and diagnostic real-time polymerase chain reaction (RT-PCR). IHC results showed Leishmania spp. in 98, 14, and 8 % of the intestines, ovaries, and salivary glands, respectively. Real-time PCR showed that 89, 41, and 33 % of the tick intestine, ovary, and salivary glands, respectively, were positive for Leishmania spp. The verification of promastigotes of Leishmania spp. by two independent techniques in ticks collected from these urban region dogs showed that there is need for clarification of the role of ticks in the transmission of canine visceral leishmaniasis in Brazil. |
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Palavras-Chave: |
Ixodids. |
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Thesagro: |
Cão; Carrapato; Leishmaniose. |
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Thesaurus Nal: |
Dogs; Immunohistochemistry; Leishmaniasis; Quantitative polymerase chain reaction. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/158208/1/Identification-of-Leishmania-spp.-promastigotes.pdf
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Marc: |
LEADER 02476naa a2200349 a 4500
001 2050014
005 2017-03-28
008 2016 bl uuuu u00u1 u #d
100 1 $aVIOL, M. A.
245 $aIdentification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs.$h[electronic resource]
260 $c2016
520 $aSand flies are recognized as the major vector of canine visceral leishmaniasis. However, in some areas of Brazil where sand flies do not occur, this disease is found in humans and dogs. There has been speculation that ticks might play a role in transmission of canine visceral leishmaniasis and the DNA of Leishmania spp. has been reported in whole ticks. We investigated the presence of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus ticks collected from tickinfested dogs in two cities of Brazil. We used 66 dogs that tested positive and 33 that tested negative for Leishmania spp. according to direct cytological examination assays. Ten ticks were collected from each dog and dissected to collect the intestines, ovaries, and salivary glands for immunohistochemistry (IHC) and diagnostic real-time polymerase chain reaction (RT-PCR). IHC results showed Leishmania spp. in 98, 14, and 8 % of the intestines, ovaries, and salivary glands, respectively. Real-time PCR showed that 89, 41, and 33 % of the tick intestine, ovary, and salivary glands, respectively, were positive for Leishmania spp. The verification of promastigotes of Leishmania spp. by two independent techniques in ticks collected from these urban region dogs showed that there is need for clarification of the role of ticks in the transmission of canine visceral leishmaniasis in Brazil.
650 $aDogs
650 $aImmunohistochemistry
650 $aLeishmaniasis
650 $aQuantitative polymerase chain reaction
650 $aCão
650 $aCarrapato
650 $aLeishmaniose
653 $aIxodids
700 1 $aGUERRERO, F. D.
700 1 $aOLIVEIRA, B. C. M. de
700 1 $aAQUINO, M. C. C. de
700 1 $aLOIOLA, S. H.
700 1 $aMELO, G. D. de
700 1 $aGOMES, A. H. de S.
700 1 $aKANAMURA, C. T.
700 1 $aGARCIA, M. V.
700 1 $aANDREOTTI, R.
700 1 $aLIMA, V. M. F. de
700 1 $aBRESCIANI, K. D. S.
773 $tParasitology Research$gv. 115, n, 9, p. 3479-3484, sept. 2016.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Clima Temperado. Para informações adicionais entre em contato com cpact.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
10/12/2008 |
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Data da última atualização: |
15/12/2008 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
GALARÇA, S. P.; TREPTOW, R. de O.; FLORES CANTILLANO, R. F.; SCHÜNEMANN, A. P. P. |
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Título: |
Influência da atmosfera modificada na aparêcia de tangerina CV. Ponkan (C. reticulata Blanco) produzida no sistema orgânico. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Anais... Frutas para todos: estratégias, tecnologias e visão sustentável. Vitória: Sociedade Brasileira de Fruticultura, 2008. 1 CD-ROM. |
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Idioma: |
Português |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00728naa a2200145 a 4500
001 1746260
005 2008-12-15
008 2008 bl uuuu u00u1 u #d
100 1 $aGALARÇA, S. P.
245 $aInfluência da atmosfera modificada na aparêcia de tangerina CV. Ponkan (C. reticulata Blanco) produzida no sistema orgânico.
260 $c2008
700 1 $aTREPTOW, R. de O.
700 1 $aFLORES CANTILLANO, R. F.
700 1 $aSCHÜNEMANN, A. P. P.
773 $tIn: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Anais... Frutas para todos: estratégias, tecnologias e visão sustentável. Vitória: Sociedade Brasileira de Fruticultura, 2008. 1 CD-ROM.
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Embrapa Clima Temperado (CPACT) |
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