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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
25/10/2017 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEGORARO, C.; STORCH, T. T.; CRIZEL, G. R.; ROMBALDI, C. V.; GIRARDI, C. L. |
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Afiliação: |
Camila Pegoraro, Brazilian Agricultural Research Corporation, Embrapa Uva e Vinho, Bento Gonçalves, Brazil; Tatiane Tim Storch, Brazilian Agricultural Research Corporation, Embrapa Uva e Vinho, Bento Gonçalves, Brazil; Gisele Rodrigues Crizel, Brazilian Agricultural Research Corporation, Embrapa Uva e Vinho, Bento Gonçalves, Brazil; Cesar Valmor Rombaldi, Federal University of Pelotas, Pelotas, Brazil; CESAR LUIS GIRARDI, CNPUV. |
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Título: |
Identification of biomarkers associated to 'Gala' apples ripening and postharvest quality . |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Comunicata Scientiae, v. 7, n. 4, p. 494-503, 2016. |
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DOI: |
10.14295/CS.v7i4.1545 |
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Idioma: |
Inglês |
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Conteúdo: |
Apple is, sociocultural and economically, on of the most important species in the world, and stands out for its high storage potential. However, the monitoring of factors that could result in fruit quality modifications during postharvest is essential to ensure the acceptability and for the development of new storage technologies in order to increase fruit shelf life. Approaches focused on molecular biology, such as RT-qPCR have been used to better understand the mechanisms involved in fruit development and maturation. In this study the use of RT-qPCR to monitoring apple quality during ripening and development in different postharvest conditions such as room temperature, cold storage with or without control of atmosphere and the 1-methylcyclopropene usage were proposed. The potential of genes involved in ethylene biosynthesis and response, cell wall modification and degradation, sugar and aroma metabolisms for employment as biomarkers of fruit development and quality were evaluated. Thus MdEXP4 is highlighted as biomarker for development and MdACO1, MdPG1, MdAF1, MdAF3 and MdAAT2 as potential biomarkers for ripening. MdACO1 and MdPG1 appear as suitable markers for quality, conservation technologies and storage time in apples. This work suggests that the study of gene expression by RT-qPCR may be an alternative for a better fruit characterization during the development and postharvest period. Keywords: Cold storage, gene expression, Malus x domestica, molecular analysis |
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Palavras-Chave: |
Amadureceimento; Análises moleculares; Armazenamento refrigerado; Biomarcadores; Expressão genética; Maçã gala; Molecular analysis. |
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Thesagro: |
Maçã; Malus doméstica; Pós-Colheita. |
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Thesaurus Nal: |
cold storage; gene expression. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165545/1/artigo-biomarcadores.pdf
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Marc: |
LEADER 02436naa a2200325 a 4500
001 2078129
005 2019-05-06
008 2016 bl uuuu u00u1 u #d
024 7 $a10.14295/CS.v7i4.1545$2DOI
100 1 $aPEGORARO, C.
245 $aIdentification of biomarkers associated to 'Gala' apples ripening and postharvest quality .$h[electronic resource]
260 $c2016
520 $aApple is, sociocultural and economically, on of the most important species in the world, and stands out for its high storage potential. However, the monitoring of factors that could result in fruit quality modifications during postharvest is essential to ensure the acceptability and for the development of new storage technologies in order to increase fruit shelf life. Approaches focused on molecular biology, such as RT-qPCR have been used to better understand the mechanisms involved in fruit development and maturation. In this study the use of RT-qPCR to monitoring apple quality during ripening and development in different postharvest conditions such as room temperature, cold storage with or without control of atmosphere and the 1-methylcyclopropene usage were proposed. The potential of genes involved in ethylene biosynthesis and response, cell wall modification and degradation, sugar and aroma metabolisms for employment as biomarkers of fruit development and quality were evaluated. Thus MdEXP4 is highlighted as biomarker for development and MdACO1, MdPG1, MdAF1, MdAF3 and MdAAT2 as potential biomarkers for ripening. MdACO1 and MdPG1 appear as suitable markers for quality, conservation technologies and storage time in apples. This work suggests that the study of gene expression by RT-qPCR may be an alternative for a better fruit characterization during the development and postharvest period. Keywords: Cold storage, gene expression, Malus x domestica, molecular analysis
650 $acold storage
650 $agene expression
650 $aMaçã
650 $aMalus doméstica
650 $aPós-Colheita
653 $aAmadureceimento
653 $aAnálises moleculares
653 $aArmazenamento refrigerado
653 $aBiomarcadores
653 $aExpressão genética
653 $aMaçã gala
653 $aMolecular analysis
700 1 $aSTORCH, T. T.
700 1 $aCRIZEL, G. R.
700 1 $aROMBALDI, C. V.
700 1 $aGIRARDI, C. L.
773 $tComunicata Scientiae$gv. 7, n. 4, p. 494-503, 2016.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| Registros recuperados : 120 | |
| 6. |  | GIRARDI, C. L. (ed.). Maçã: pós-colheita. Brasília, DF: Embrapa Informação Tecnológica; Bento Gonçalves: Embrapa Uva e Vinho, 2004. 109 p. il. (Frutas do Brasil, 39).| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
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| Registros recuperados : 120 | |
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