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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
29/02/2008 |
Data da última atualização: |
05/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
EGITO, A. S. do; GIRARDET, J. -M.; LAGUNA, L. E.; POIRSON, C.; MOLLÉ, D.; MICLO, L.; HUMBERT, G.; GAILLARD, J. -L. |
Afiliação: |
ANTONIO SILVIO DO EGITO, CNPC; J.-M. Girardet, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); LUIS EDUARDO LAGUNA, CNPC; C. Poirson, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); D. Mollé, Institut National de la Recherche Agronomique; L. Miclo, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); G. Humbert, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); J.-L. Gaillard, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA). |
Título: |
Milk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of bovine k-casein. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
International Dairy Journal, v. 17, n. 7, p. 816-825, Jul. 2007. |
DOI: |
10.1016/j.idairyj.2006.09.012 |
Idioma: |
Inglês |
Conteúdo: |
Milk-clotting activity found in ammonium sulfate-precipitated protein extracts from Albizia lebbeck and Helianthus annuus seeds was studied. Specific clotting activity of albizia seed extract was 15 times higher than that of sunflower seed extract. Zymogram analysis revealed several proteolytic bands in albizia seed extract and one diffuse proteolytic band for sunflower seed extract. Whole bovine casein was incubated with the plant seed extracts or chymosin and some breakdown products were characterized by reversed-phase high-performance liquid chromatography and electrophoresis. Similar to chymosin, the two seed extracts exhibited proteolytic activity toward ?-casein, ?s-casein and ?-casein, with the highest activity observed for the albizia seed extract. Mass spectrometry analysis showed that the sunflower extract hydrolyzed ?-casein at the Phe105?Met106 bond, as does chymosin. The albizia extract also displayed activity on ?-casein, but the Lys116?Thr117 bond was its preferred target. |
Palavras-Chave: |
Bovine casein; Cheese; Enzima coagulante; Plant rennet; Sunflower. |
Thesagro: |
Albizia lebbeck; Caseína; Coagulação; Coalho; Extrato Vegetal; Leite. |
Thesaurus Nal: |
Albizia; Aspartic proteinases; Chymosin; Cynara cardunculus; Kappa-casein; Proteolysis; Sheep. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02194naa a2200433 a 4500 001 1514197 005 2019-04-05 008 2007 bl uuuu u00u1 u #d 024 7 $a10.1016/j.idairyj.2006.09.012$2DOI 100 1 $aEGITO, A. S. do 245 $aMilk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of bovine k-casein.$h[electronic resource] 260 $c2007 520 $aMilk-clotting activity found in ammonium sulfate-precipitated protein extracts from Albizia lebbeck and Helianthus annuus seeds was studied. Specific clotting activity of albizia seed extract was 15 times higher than that of sunflower seed extract. Zymogram analysis revealed several proteolytic bands in albizia seed extract and one diffuse proteolytic band for sunflower seed extract. Whole bovine casein was incubated with the plant seed extracts or chymosin and some breakdown products were characterized by reversed-phase high-performance liquid chromatography and electrophoresis. Similar to chymosin, the two seed extracts exhibited proteolytic activity toward ?-casein, ?s-casein and ?-casein, with the highest activity observed for the albizia seed extract. Mass spectrometry analysis showed that the sunflower extract hydrolyzed ?-casein at the Phe105?Met106 bond, as does chymosin. The albizia extract also displayed activity on ?-casein, but the Lys116?Thr117 bond was its preferred target. 650 $aAlbizia 650 $aAspartic proteinases 650 $aChymosin 650 $aCynara cardunculus 650 $aKappa-casein 650 $aProteolysis 650 $aSheep 650 $aAlbizia lebbeck 650 $aCaseína 650 $aCoagulação 650 $aCoalho 650 $aExtrato Vegetal 650 $aLeite 653 $aBovine casein 653 $aCheese 653 $aEnzima coagulante 653 $aPlant rennet 653 $aSunflower 700 1 $aGIRARDET, J. -M. 700 1 $aLAGUNA, L. E. 700 1 $aPOIRSON, C. 700 1 $aMOLLÉ, D. 700 1 $aMICLO, L. 700 1 $aHUMBERT, G. 700 1 $aGAILLARD, J. -L. 773 $tInternational Dairy Journal$gv. 17, n. 7, p. 816-825, Jul. 2007.
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Embrapa Caprinos e Ovinos (CNPC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
21/11/2022 |
Data da última atualização: |
28/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
BORGES, L. M.; PASTORE, J. F. B.; SOUZA, A. F. C.; PIRANI, J. R.; SIMON, M. F. |
Afiliação: |
LEONARDO M. BORGES, Universidade Federal de São Carlos; JOSÉ FLORIANO B. PASTORE, Universidade Federal de Santa Catarina; ADRIANA F. C. SOUZA, Instituto Geral de Perícias; JOSÉ RUBENS PIRANI, Universidade de São Paulo; MARCELO FRAGOMENI SIMON, Cenargen. |
Título: |
Let there be clades: phylogenetics of Mimosa series Pachycarpae and Setosae (Fabaceae) improves the infrageneric classification of the genus. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Botanical Journal of the Linnean Society, v. 201, n. 1, p. 61-79, 2023. |
DOI: |
https://doi.org/10.1093/botlinnean/boac029 |
Idioma: |
Inglês |
Palavras-Chave: |
Campos rupestres; Dynamic homology; Large genera. |
Thesagro: |
Leguminosae. |
Thesaurus NAL: |
Taxonomy. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00779naa a2200229 a 4500 001 2148508 005 2023-09-28 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1093/botlinnean/boac029$2DOI 100 1 $aBORGES, L. M. 245 $aLet there be clades$bphylogenetics of Mimosa series Pachycarpae and Setosae (Fabaceae) improves the infrageneric classification of the genus.$h[electronic resource] 260 $c2023 650 $aTaxonomy 650 $aLeguminosae 653 $aCampos rupestres 653 $aDynamic homology 653 $aLarge genera 700 1 $aPASTORE, J. F. B. 700 1 $aSOUZA, A. F. C. 700 1 $aPIRANI, J. R. 700 1 $aSIMON, M. F. 773 $tBotanical Journal of the Linnean Society$gv. 201, n. 1, p. 61-79, 2023.
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