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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
18/12/2017 |
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Data da última atualização: |
18/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BELESINI, A. A.; CARVALHO, F. M. S.; TELLES, B. R.; CASTRO, G. M. de; GIACHETTO, P. F.; VANTINI, J. S.; CARLIN, S. D.; CAZETTA, J. O.; PINHEIRO, D. G.; FERRO, M. I. T. |
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Afiliação: |
FCAV/Unesp; FCAV/Unesp; FCAV/Unesp; POLIANA FERNANDA GIACHETTO, CNPTIA; FCAV/Unesp; Centro Avançado da Pesquisa Tecnológica do Agronegócio de Cana, Ribeirão Preto, SP; FCAV/Unesp; FCAV/Unesp; FCAV/Unesp. |
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Título: |
De novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 2, p. 1-20, 2017. |
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DOI: |
http://dx.doi.org/10.4238/gmr16028845 |
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Idioma: |
Inglês |
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Notas: |
Article gmr16028845. |
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Conteúdo: |
ABSTRACT. Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and drought-sensitive cultivar (RB855453). For this purpose, plants were grown in a greenhouse for 60 days and were then submitted to three treatments: control (-0.01 to -0.015 MPa), moderate water deficit (-0.05 to -0.055 MPa), and severe water deficit (-0.075 to -0.08 MPa). The plants were evaluated 30, 60, and 90 days after the beginning of treatment. Sequencing on an Illumina platform (RNA-Seq) generated more than one billion sequences, resulting in 177,509 and 185,153 transcripts for the tolerant and sensitive cultivar, respectively. These transcripts were aligned with sequences from Saccharum spp, Sorghum bicolor, Miscanthus giganteus, and Arabidopsis thaliana available in public databases. The differentially expressed genes detected during the prolonged period of water deficit permit to increase our understanding of the molecular patterns involved in the physiological response of the two cultivars. The tolerant cultivar differentially expressed a larger number of genes at 90 days, while in the sensitive cultivar the number of differentially expressed genes was higher in 30 days. Both cultivars perceived the lack of water, but the tolerant cultivar responded more slowly than the sensitive cultivar. The latter requires rapid activation of different water-deficit stress response mechanisms for its survival. This rapid activation of metabolic pathways in response to water stress does not appear to be the key mechanism of drought tolerance in sugarcane. There is still much to clarify on the molecular and physiological pattern of plants in response to drought. MenosABSTRACT. Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and drought-sensitive cultivar (RB855453). For this purpose, plants were grown in a greenhouse for 60 days and were then submitted to three treatments: control (-0.01 to -0.015 MPa), moderate water deficit (-0.05 to -0.055 MPa), and severe water deficit (-0.075 to -0.08 MPa). The plants were evaluated 30, 60, and 90 days after the beginning of treatment. Sequencing on an Illumina platform (RNA-Seq) generated more than one billion sequences, resulting in 177,509 and 185,153 transcripts for the tolerant and sensitive cultivar, respectively. These transcripts were aligned with sequences from Saccharum spp, Sorghum bicolor, Miscanthus giganteus, and Arabidopsis thaliana available in public databases. The differentially expressed genes detected during the prolonged period of water deficit permit to increase our understanding of the molecular patterns involved in the physiological response of the two cultivars. The tolerant cultivar differentially expressed a larger number of genes at 90 days, while in the sensitive cultivar the number of differentially expressed genes was higher in 30 da... Mostrar Tudo |
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Palavras-Chave: |
Expressão genética. |
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Thesagro: |
Cana de açúcar; Deficiência hídrica. |
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Thesaurus Nal: |
Drought; Gene expression; Saccharum; Sugarcane; Water stress. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169187/1/AP-Denovo-Belesini-gmr.pdf
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Marc: |
LEADER 03036naa a2200349 a 4500
001 2082783
005 2017-12-18
008 2017 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr16028845$2DOI
100 1 $aBELESINI, A. A.
245 $aDe novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress.$h[electronic resource]
260 $c2017
500 $aArticle gmr16028845.
520 $aABSTRACT. Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and drought-sensitive cultivar (RB855453). For this purpose, plants were grown in a greenhouse for 60 days and were then submitted to three treatments: control (-0.01 to -0.015 MPa), moderate water deficit (-0.05 to -0.055 MPa), and severe water deficit (-0.075 to -0.08 MPa). The plants were evaluated 30, 60, and 90 days after the beginning of treatment. Sequencing on an Illumina platform (RNA-Seq) generated more than one billion sequences, resulting in 177,509 and 185,153 transcripts for the tolerant and sensitive cultivar, respectively. These transcripts were aligned with sequences from Saccharum spp, Sorghum bicolor, Miscanthus giganteus, and Arabidopsis thaliana available in public databases. The differentially expressed genes detected during the prolonged period of water deficit permit to increase our understanding of the molecular patterns involved in the physiological response of the two cultivars. The tolerant cultivar differentially expressed a larger number of genes at 90 days, while in the sensitive cultivar the number of differentially expressed genes was higher in 30 days. Both cultivars perceived the lack of water, but the tolerant cultivar responded more slowly than the sensitive cultivar. The latter requires rapid activation of different water-deficit stress response mechanisms for its survival. This rapid activation of metabolic pathways in response to water stress does not appear to be the key mechanism of drought tolerance in sugarcane. There is still much to clarify on the molecular and physiological pattern of plants in response to drought.
650 $aDrought
650 $aGene expression
650 $aSaccharum
650 $aSugarcane
650 $aWater stress
650 $aCana de açúcar
650 $aDeficiência hídrica
653 $aExpressão genética
700 1 $aCARVALHO, F. M. S.
700 1 $aTELLES, B. R.
700 1 $aCASTRO, G. M. de
700 1 $aGIACHETTO, P. F.
700 1 $aVANTINI, J. S.
700 1 $aCARLIN, S. D.
700 1 $aCAZETTA, J. O.
700 1 $aPINHEIRO, D. G.
700 1 $aFERRO, M. I. T.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 16, n. 2, p. 1-20, 2017.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
17/09/2013 |
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Data da última atualização: |
01/08/2014 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OLIVEIRA, A. S. de; RETORE, M.; LAZARI, G. G. de; SILVA, W. M.; TOMAZI, M. |
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Afiliação: |
AFONSO SIQUEIRA DE OLIVEIRA, UEMS; MARCIANA RETORE, CPAO; GISLAINE GOMES DE LAZARI, UNIGRAN; WILLIAM MARRA SILVA, CPAO; MICHELY TOMAZI, CPAO. |
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Título: |
Metodologia modificada para determinação de óxido de titânio como marcador em fezes de bovinos. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2013, Dourados. Resumos... Dourados: Embrapa Agropecuária Oeste, 2013. 1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
JIPE 2013. |
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Palavras-Chave: |
Reagent; Reagente. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/89566/1/17.pdf
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Marc: |
LEADER 00620nam a2200181 a 4500
001 1966455
005 2014-08-01
008 2013 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, A. S. de
245 $aMetodologia modificada para determinação de óxido de titânio como marcador em fezes de bovinos.
260 $aIn: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2013, Dourados. Resumos... Dourados: Embrapa Agropecuária Oeste, 2013. 1 CD-ROM.$c2013
500 $aJIPE 2013.
653 $aReagent
653 $aReagente
700 1 $aRETORE, M.
700 1 $aLAZARI, G. G. de
700 1 $aSILVA, W. M.
700 1 $aTOMAZI, M.
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Embrapa Agropecuária Oeste (CPAO) |
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