Data/hora: 03/06/2024 21:44:00

Biblioteca(s): 	Embrapa Agricultura Digital; Embrapa Café.	
Data corrente: 	05/02/2015	
Data da última atualização: 	06/02/2015	
Autoria: 	CARDOSO, D. C.; MARTINATI, J. C.; GIACHETTO, P. F.; VIDAL, R. O.; CARAZZOLLE, M. F.; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P.	
Afiliação: 	DANIELLE C. CARDOSO, IAC; JULIANA C MARTINATI, IAC; POLIANA FERNANDA GIACHETTO, CNPTIA; RAMON O. VIDAL; MARCELO F. CARAZZOLLE; LILIAN PADILHA, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC; MIRIAN PEREZ MALUF, SAPC.	
Título: 	Large-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection.	
Ano de publicação: 	2014	
Fonte/Imprenta: 	BMC Genomics, London, v. 15, p. 1-20, 2014.	
DOI: 	10.1186/1471-2164-15-66	
Idioma: 	Inglês	
Conteúdo: 	A successful development of herbivorous insects into plant tissues depends on coordination of metabolic processes. Plants have evolved complex mechanisms to recognize such attacks, and to trigger a defense response. To understand the transcriptional basis of this response, we compare gene expression profiles of two coffee genotypes, susceptible and resistant to leaf miner (Leucoptera coffella). A total of 22000 EST sequences from the Coffee Genome Database were selected for a microarray analysis. Fluorescence probes were synthesized using mRNA from the infested and non-infested coffee plants. Array hybridization, scanning and data normalization were performed using Nimble Scan® e ArrayStar® platforms. Genes with foldchange values +/-2 were considered differentially expressed. A validation of 18 differentially expressed genes was performed in infected plants using qRT-PCR approach.	
Palavras-Chave: 	Microarranjo; Plant defense.	
Thesagro: 	Café; Coffea Arábica.	
Thesaurus Nal: 	Genes; Leafminers; Microarray technology.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/117310/1/Large-scale-analysis.pdf	
Registro original: 	Embrapa Agricultura Digital (CNPTIA)	
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