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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
15/10/2015 |
Data da última atualização: |
15/03/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GODOY, T. F.; MOREIRA, G. C. M.; BOSCHIERO, C.; GHEYAS, A. A.; GASPARIN, G.; PADUAN, M.; ANDRADE, S. C. S.; MONTENEGRO, H.; BURT, D. W.; LEDUR, M. C.; COUTINHO, L. L. |
Afiliação: |
THAYS FERNANDA GODOY, ESALQ; GABRIEL COSTA MONTEIRO MOREIRA, ESALQ; CLARISSA BOSCHIERO, ESALQ; ALMAS A. GHEYAS, University of Edinburgh; GUSTAVO GASPARIN, ESALQ; MARCELA PADUAN, ESALQ; SONIA CRISTINA DA SILVA ANDRADE, ESALQ; HORACIO MONTENEGRO, ESALQ; DAVID W. BURT, University of Edinburgh; MONICA CORREA LEDUR, CNPSA; LUIZ LEHMANN COUTINHO, ESALQ. |
Título: |
SNP and INDEL detection in a QTL region on chicken chromosome 2 associated with muscle deposition. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Animal Genetics, v. 6, n. 2, p. 158-163, 2015. |
DOI: |
10.1111/age.12271 |
Idioma: |
Inglês |
Conteúdo: |
Genetic improvement is important for the poultry industry, contributing to increased efficiency of meat production and quality. Because breast muscle is the most valuable part of the chicken carcass, knowledge of polymorphisms influencing this trait can help breeding programs. Therefore, the complete genome of 18 chickens from two different experimental lines (broiler and layer) from EMBRAPA was sequenced, and SNPs and INDELs were detected in a QTL region for breast muscle deposition on chicken chromosome 2 between microsatellite markers MCW0185 and MCW0264 (105 849?112 649 kb). Initially, 94 674 unique SNPs and 10 448 unique INDELs were identified in the target region. After quality filtration, 77% of the SNPs (85 765) and 60% of the INDELs (7828) were retained. The studied region contains 66 genes, and functional annotation of the filtered variants identified 517 SNPs and three INDELs in exonic regions. Of these, 357 SNPs were classified as synonymous, 153 as nonsynonymous, three as stopgain, four INDELs as frameshift and three INDELs as nonframeshift. These exonic mutations were identified in 37 of the 66 genes from the target region, three of which are related to muscle development (DTNA, RB1CC1 and MOS). Fifteen non-tolerated SNPs were detected in several genes (MEP1B, PRKDC, NSMAF, TRAPPC8, SDR16C5, CHD7, ST18 and RB1CC1). These loss-of-function and exonic variants present in genes related to muscle development can be considered candidate variants for further studies in chickens. Further association studies should be performed with these candidate mutations as should validation in commercial populations to allow a better explanation of QTL effects. MenosGenetic improvement is important for the poultry industry, contributing to increased efficiency of meat production and quality. Because breast muscle is the most valuable part of the chicken carcass, knowledge of polymorphisms influencing this trait can help breeding programs. Therefore, the complete genome of 18 chickens from two different experimental lines (broiler and layer) from EMBRAPA was sequenced, and SNPs and INDELs were detected in a QTL region for breast muscle deposition on chicken chromosome 2 between microsatellite markers MCW0185 and MCW0264 (105 849?112 649 kb). Initially, 94 674 unique SNPs and 10 448 unique INDELs were identified in the target region. After quality filtration, 77% of the SNPs (85 765) and 60% of the INDELs (7828) were retained. The studied region contains 66 genes, and functional annotation of the filtered variants identified 517 SNPs and three INDELs in exonic regions. Of these, 357 SNPs were classified as synonymous, 153 as nonsynonymous, three as stopgain, four INDELs as frameshift and three INDELs as nonframeshift. These exonic mutations were identified in 37 of the 66 genes from the target region, three of which are related to muscle development (DTNA, RB1CC1 and MOS). Fifteen non-tolerated SNPs were detected in several genes (MEP1B, PRKDC, NSMAF, TRAPPC8, SDR16C5, CHD7, ST18 and RB1CC1). These loss-of-function and exonic variants present in genes related to muscle development can be considered candidate variants for further studies i... Mostrar Tudo |
Palavras-Chave: |
Músculo de peito; SNPs. |
Thesagro: |
Frango; Melhoramento genético animal; Mutação. |
Thesaurus Nal: |
Animal genetics; Breast muscle; Broiler chickens; Genetic polymorphism; Quantitative trait loci; Single nucleotide polymorphism. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02784naa a2200385 a 4500 001 2026548 005 2016-03-15 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1111/age.12271$2DOI 100 1 $aGODOY, T. F. 245 $aSNP and INDEL detection in a QTL region on chicken chromosome 2 associated with muscle deposition.$h[electronic resource] 260 $c2015 520 $aGenetic improvement is important for the poultry industry, contributing to increased efficiency of meat production and quality. Because breast muscle is the most valuable part of the chicken carcass, knowledge of polymorphisms influencing this trait can help breeding programs. Therefore, the complete genome of 18 chickens from two different experimental lines (broiler and layer) from EMBRAPA was sequenced, and SNPs and INDELs were detected in a QTL region for breast muscle deposition on chicken chromosome 2 between microsatellite markers MCW0185 and MCW0264 (105 849?112 649 kb). Initially, 94 674 unique SNPs and 10 448 unique INDELs were identified in the target region. After quality filtration, 77% of the SNPs (85 765) and 60% of the INDELs (7828) were retained. The studied region contains 66 genes, and functional annotation of the filtered variants identified 517 SNPs and three INDELs in exonic regions. Of these, 357 SNPs were classified as synonymous, 153 as nonsynonymous, three as stopgain, four INDELs as frameshift and three INDELs as nonframeshift. These exonic mutations were identified in 37 of the 66 genes from the target region, three of which are related to muscle development (DTNA, RB1CC1 and MOS). Fifteen non-tolerated SNPs were detected in several genes (MEP1B, PRKDC, NSMAF, TRAPPC8, SDR16C5, CHD7, ST18 and RB1CC1). These loss-of-function and exonic variants present in genes related to muscle development can be considered candidate variants for further studies in chickens. Further association studies should be performed with these candidate mutations as should validation in commercial populations to allow a better explanation of QTL effects. 650 $aAnimal genetics 650 $aBreast muscle 650 $aBroiler chickens 650 $aGenetic polymorphism 650 $aQuantitative trait loci 650 $aSingle nucleotide polymorphism 650 $aFrango 650 $aMelhoramento genético animal 650 $aMutação 653 $aMúsculo de peito 653 $aSNPs 700 1 $aMOREIRA, G. C. M. 700 1 $aBOSCHIERO, C. 700 1 $aGHEYAS, A. A. 700 1 $aGASPARIN, G. 700 1 $aPADUAN, M. 700 1 $aANDRADE, S. C. S. 700 1 $aMONTENEGRO, H. 700 1 $aBURT, D. W. 700 1 $aLEDUR, M. C. 700 1 $aCOUTINHO, L. L. 773 $tAnimal Genetics$gv. 6, n. 2, p. 158-163, 2015.
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Embrapa Suínos e Aves (CNPSA) |
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Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
17/01/2011 |
Data da última atualização: |
29/03/2012 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA, J. B. da S.; CARRÃO-PANIZZI, M.C.; FONSECA, F. J.; PRUDÊNCIO, S. H. |
Afiliação: |
JOSEMEYRE BONIFÁCIO DA SILVA, UEL; MERCEDES CONCORDIA CARRAO PANIZZI, CNPSO; FERNANDO JOSEPETT FONSECA, Escola Politécnica da USP; SANDRA HELENA PRUDÊNCIO, UEL. |
Título: |
Flavor of soybean cultivars: sensory descriptive analysis and eletronic tongue. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES, 1., 2010, São Pedro, SP. [Anais...] São Pedro: Rede AgroNano: Aptor Software, 2010. Abstract, C515. 1 CD-ROM. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas. NanoAgri 2010. |
Idioma: |
Inglês |
Conteúdo: |
Soybean cultivars have different uses and are classified as food type and grain type. Grain type cultivars are for oil processing and animal feeding and present ?beany" flavor. Food type cultivars are those for human consumption that have special chemical, physical and sensory characteristics. Through sensory evaluation, it was possible to identify different flavors for five distinct special soybean cultivars: BRS 213 (null lipoxygenases), BRS 216 (small grain size), BRS 267 (vegetable type), BRS 133 and BRS 258 (grain type cultivars).The objective of this work was to study the sensory characteristics of different soybean cultivars using the Quantitative Descriptive Analysis (QDA) by Stone and Sidel, 2004 [1] and the electronic tongue fabricated by self-assembly technique employing an automatic deposition of films developed at Escola Politécnica - USP. Data was analysed by Principal Component Analysis (PCA) using the STATISTICA program version 6.0 (2001) and results showed that, electronic sensors were effective in distinguishing and separating soybean cultivars in five different groups as the evaluation of the trained sensory panel. In Figure 1 it can be observed the comparing graphics of the PCA data obtained by QDA with the electronic tongue, where the separation of soybean cultivars is distingueshed. Therefore the use of the electronic tongue in food assessment can provide a rapid and accurate method to analyse flavor of food products, and lines for breeding purposes. |
Palavras-Chave: |
Nanotecnologia. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/56638/1/flavor.mercedes.nanoagri2010.pdf
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Marc: |
LEADER 02254nam a2200157 a 4500 001 1873321 005 2012-03-29 008 2010 bl uuuu u00u1 u #d 100 1 $aSILVA, J. B. da S. 245 $aFlavor of soybean cultivars$bsensory descriptive analysis and eletronic tongue. 260 $aIn: In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES, 1., 2010, São Pedro, SP. [Anais...] São Pedro: Rede AgroNano: Aptor Software, 2010. Abstract, C515. 1 CD-ROM. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas. NanoAgri 2010.$c2010 520 $aSoybean cultivars have different uses and are classified as food type and grain type. Grain type cultivars are for oil processing and animal feeding and present ?beany" flavor. Food type cultivars are those for human consumption that have special chemical, physical and sensory characteristics. Through sensory evaluation, it was possible to identify different flavors for five distinct special soybean cultivars: BRS 213 (null lipoxygenases), BRS 216 (small grain size), BRS 267 (vegetable type), BRS 133 and BRS 258 (grain type cultivars).The objective of this work was to study the sensory characteristics of different soybean cultivars using the Quantitative Descriptive Analysis (QDA) by Stone and Sidel, 2004 [1] and the electronic tongue fabricated by self-assembly technique employing an automatic deposition of films developed at Escola Politécnica - USP. Data was analysed by Principal Component Analysis (PCA) using the STATISTICA program version 6.0 (2001) and results showed that, electronic sensors were effective in distinguishing and separating soybean cultivars in five different groups as the evaluation of the trained sensory panel. In Figure 1 it can be observed the comparing graphics of the PCA data obtained by QDA with the electronic tongue, where the separation of soybean cultivars is distingueshed. Therefore the use of the electronic tongue in food assessment can provide a rapid and accurate method to analyse flavor of food products, and lines for breeding purposes. 653 $aNanotecnologia 700 1 $aCARRÃO-PANIZZI, M.C. 700 1 $aFONSECA, F. J. 700 1 $aPRUDÊNCIO, S. H.
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