Data/hora: 07/06/2024 02:07:29

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 06/10/1993
Data da última atualização: | 06/10/1993
Autoria: | JARRET, R. L.; VUYLSTEKE, D. R.; GAWEL, N. J.; PIMENTEL, R. B.; DUNBAR, L. J.
Afiliação: | United States Department of Agriculture, Agricultural Research Service, Regional Plant Introduction Station, 1109 Experiment Street, Griffin, GA 30223 USA (address for offprints).
Título: | Detecting genetic diversity in diploid bananas using PCR and primers from a highly respectitive DNA sequence.
Ano de publicação: | 1993
Fonte/Imprenta: | Euphytica International Journal of Plant Breeding, v.68, n.1-2, 1993.
ISSN: | 0014-2336
Idioma: | Inglês
Conteúdo: | The polymerase chain reaction (PCR) was used to detect polymorphisms among 29 diploid clones of Musa acuminata Colla. from Papua New Guinea. Primer sequences were derived from a 520 bp highly respectitive DNA sequence isolated from M.acuminata ssp. malaccensis. Primers, used individually, detected a total of 48 polymorphisms that were scored as unit characters and used to generate a Jaccard's similarity index. Principal coordinate analysis (PCO) was used to cluster clones and the unweighted paired-group method of analysis (UPGMA) was used to compute genetic distance among the materials examined. The abundance of diversity within the PNG diploids examined reflects the exterme genetic variability within the M.acuminata gene pool. PCR,utilizing primes from a highly respetitive sequence, is a rapid means of detecting genetic diversity in M. acuminata.
Palavras-Chave: | Diploid banana; PCR; Plant germplasm; Repetitive sequence.
Thesagro: | Musa Acuminata.
Thesaurus Nal: | Papua New Guinea; polymerase chain reaction.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (2271-1) - ADC - AP - -- - 575.05 -  - 575.05 - DIS