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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
27/06/1996 |
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Data da última atualização: |
04/04/2017 |
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Autoria: |
EAGLESOME, M. D.; SAMPATH, M. I.; GARCIA, M. M. |
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Título: |
A detection assay for Campylobcter fetus in bovine semen by restriction analysis of PCR amplified DNA. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Veterinary Research Communications, v.19, n.4, p.253-263, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
A rapid screening assay for Campylobacter fetus in bull semen was developed using the polymerase chain reaction (PCR) and restriction endonuclease analysis (REA) to complement isolation by culture. An oligonucleotide primer par (C1/C2) from the hypervariable region of 16S rRNA of C. fetus was used to amplify a 362 base pair fragment by PCR. The PCR/REA assay, which is completed in 10 hours, detected as few as three C. fetus subsp. venerealis cells in experimentally infected raw bull smenen and in semen diluted with milk or egg yolk Tris (EYT). All the strains tested, of both subspecies of C. fetus, were amplified, as were some other Campylobacter species. Restrictring the amplified products by AluI differentiated C. fetus from the other organisms. There was no visible product generated by PCR from S. sputorum subsp. bubulus, a saprophytic organim found in the prepuce of bulls, or from seven other species of bacteria found in semen. A modification of the PCR assay, using another primer pair (C3/C2) and two temeprature PCR cycling conditions, increased the probability of detecting C. fetus sunsp. venereallis. PCR amplification followed by REA could be used to screen bovine semen rapidly for C. fetus. In most cases, sequencing of C1/C2 PCR generated products would be preferable for distinguishing between the two subspecies of C. fetus. |
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Palavras-Chave: |
Bovine; Bull; PCR. |
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Thesagro: |
Bovino; Campylobacter Fetus; Sêmen; Touro. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01976naa a2200229 a 4500
001 1789228
005 2017-04-04
008 1995 bl --- 0-- u #d
100 1 $aEAGLESOME, M. D.
245 $aA detection assay for Campylobcter fetus in bovine semen by restriction analysis of PCR amplified DNA.
260 $c1995
520 $aA rapid screening assay for Campylobacter fetus in bull semen was developed using the polymerase chain reaction (PCR) and restriction endonuclease analysis (REA) to complement isolation by culture. An oligonucleotide primer par (C1/C2) from the hypervariable region of 16S rRNA of C. fetus was used to amplify a 362 base pair fragment by PCR. The PCR/REA assay, which is completed in 10 hours, detected as few as three C. fetus subsp. venerealis cells in experimentally infected raw bull smenen and in semen diluted with milk or egg yolk Tris (EYT). All the strains tested, of both subspecies of C. fetus, were amplified, as were some other Campylobacter species. Restrictring the amplified products by AluI differentiated C. fetus from the other organisms. There was no visible product generated by PCR from S. sputorum subsp. bubulus, a saprophytic organim found in the prepuce of bulls, or from seven other species of bacteria found in semen. A modification of the PCR assay, using another primer pair (C3/C2) and two temeprature PCR cycling conditions, increased the probability of detecting C. fetus sunsp. venereallis. PCR amplification followed by REA could be used to screen bovine semen rapidly for C. fetus. In most cases, sequencing of C1/C2 PCR generated products would be preferable for distinguishing between the two subspecies of C. fetus.
650 $aBovino
650 $aCampylobacter Fetus
650 $aSêmen
650 $aTouro
653 $aBovine
653 $aBull
653 $aPCR
700 1 $aSAMPATH, M. I.
700 1 $aGARCIA, M. M.
773 $tVeterinary Research Communications$gv.19, n.4, p.253-263, 1995.
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Registro original: |
Embrapa Pantanal (CPAP) |
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| Registros recuperados : 119 | |
| 6. |  | LEITE, J.; MARTINS, L. M. V.; RUMJANEK, N. G.; XAVIER, G. R. Caracterização de bactérias isoladas de nódulos de feijão caupi em solos do semi-árido, Brasil. In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações. Seropédica: Embrapa Agrobiologia, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
|   |
| 7. | | MORGADO, L. B.; MARTINS, L. M. V.; XAVIER, G. R.; RUMJANEK, N. G. Avaliação do potencial de estirpes de rizóbio em fixar nitrogênio associadas ao feijão-caupi em Petrolina-PE. In: CONGRESSO NACIONAL DE FEIJÃO- CAUPI, 1.; REUNIÃO NACIONAL DE FEIJÃO- CAUPI, 6., 2006, Teresina, PI. Tecnologias para o agronegócio: anais. Teresina: Embrapa Meio-Norte, 2006. 1 CD-ROM (Embrapa Meio-Norte. Documentos, 121).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
|  |
| 9. | | XAVIER, G. R.; MARTINS, L. M. V; RUMJANEK, N. G.; FREIRE FILHO, F. R. Variabilidade genética em acessos de caupi analisada por meio de marcadores RAPD. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 4, p. 353-359, abr. 2005. Cowpea genetic variability analyzed by RAPD markers.| Biblioteca(s): Embrapa Agrobiologia. |
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| 16. | | LEITE, J.; XAVIER, G. R.; RUMJANEK, N. G.; MARTINS, L. M. V. Potencial biotecnológico de bactérias isoladas de nódulos de feijão-caupi para solubilizar fosfato de cálcio. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa: SBCS, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 119 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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