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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
27/06/1996 |
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Data da última atualização: |
04/04/2017 |
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Autoria: |
EAGLESOME, M. D.; SAMPATH, M. I.; GARCIA, M. M. |
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Título: |
A detection assay for Campylobcter fetus in bovine semen by restriction analysis of PCR amplified DNA. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Veterinary Research Communications, v.19, n.4, p.253-263, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
A rapid screening assay for Campylobacter fetus in bull semen was developed using the polymerase chain reaction (PCR) and restriction endonuclease analysis (REA) to complement isolation by culture. An oligonucleotide primer par (C1/C2) from the hypervariable region of 16S rRNA of C. fetus was used to amplify a 362 base pair fragment by PCR. The PCR/REA assay, which is completed in 10 hours, detected as few as three C. fetus subsp. venerealis cells in experimentally infected raw bull smenen and in semen diluted with milk or egg yolk Tris (EYT). All the strains tested, of both subspecies of C. fetus, were amplified, as were some other Campylobacter species. Restrictring the amplified products by AluI differentiated C. fetus from the other organisms. There was no visible product generated by PCR from S. sputorum subsp. bubulus, a saprophytic organim found in the prepuce of bulls, or from seven other species of bacteria found in semen. A modification of the PCR assay, using another primer pair (C3/C2) and two temeprature PCR cycling conditions, increased the probability of detecting C. fetus sunsp. venereallis. PCR amplification followed by REA could be used to screen bovine semen rapidly for C. fetus. In most cases, sequencing of C1/C2 PCR generated products would be preferable for distinguishing between the two subspecies of C. fetus. |
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Palavras-Chave: |
Bovine; Bull; PCR. |
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Thesagro: |
Bovino; Campylobacter Fetus; Sêmen; Touro. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01976naa a2200229 a 4500
001 1789228
005 2017-04-04
008 1995 bl --- 0-- u #d
100 1 $aEAGLESOME, M. D.
245 $aA detection assay for Campylobcter fetus in bovine semen by restriction analysis of PCR amplified DNA.
260 $c1995
520 $aA rapid screening assay for Campylobacter fetus in bull semen was developed using the polymerase chain reaction (PCR) and restriction endonuclease analysis (REA) to complement isolation by culture. An oligonucleotide primer par (C1/C2) from the hypervariable region of 16S rRNA of C. fetus was used to amplify a 362 base pair fragment by PCR. The PCR/REA assay, which is completed in 10 hours, detected as few as three C. fetus subsp. venerealis cells in experimentally infected raw bull smenen and in semen diluted with milk or egg yolk Tris (EYT). All the strains tested, of both subspecies of C. fetus, were amplified, as were some other Campylobacter species. Restrictring the amplified products by AluI differentiated C. fetus from the other organisms. There was no visible product generated by PCR from S. sputorum subsp. bubulus, a saprophytic organim found in the prepuce of bulls, or from seven other species of bacteria found in semen. A modification of the PCR assay, using another primer pair (C3/C2) and two temeprature PCR cycling conditions, increased the probability of detecting C. fetus sunsp. venereallis. PCR amplification followed by REA could be used to screen bovine semen rapidly for C. fetus. In most cases, sequencing of C1/C2 PCR generated products would be preferable for distinguishing between the two subspecies of C. fetus.
650 $aBovino
650 $aCampylobacter Fetus
650 $aSêmen
650 $aTouro
653 $aBovine
653 $aBull
653 $aPCR
700 1 $aSAMPATH, M. I.
700 1 $aGARCIA, M. M.
773 $tVeterinary Research Communications$gv.19, n.4, p.253-263, 1995.
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Registro original: |
Embrapa Pantanal (CPAP) |
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| 9. |  | ROMBKE, J.; GARCIA, M. Earthworms. In: SHIFT PROJECT ENV 52 (Manaus-AB). Soil fauna and litter decomposition in primary and secondary forests and a mixed culture system in Amazonia. Manaus: SMNK/Embrapa Amazonia Ocidental, 1999. p.152-167 Final Report 1996-1999.| Tipo: Capítulo em Livro Técnico-Científico |
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