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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
15/03/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERNANDES, M. S.; GARCIA, L. C.; NASCIMENTO, R. C.; SANTANA, H.; MORAIS, P. B.; CAPDEVILLE, G. de; BRASIL, B. dos S. A. F. |
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Afiliação: |
Maiara S. Fernandes; LORENA COSTA GARCIA CALSING, CNPAE; Rodrigo C. Nascimento; Hugo Santana; Priscila B. Morais; GUY DE CAPDEVILLE, CNPAE; BRUNO DOS SANTOS A F BRASIL, SPD. |
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Título: |
Customized cryopreservation protocols for chlorophytes based on cell morphology. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Algal Research, v. 38, 2019. |
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Idioma: |
Português |
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Conteúdo: |
The development of efficient algae cryopreservation methods is pivotal to the establishment of long-term culture collections as well as algae breeding and genetic modification programs. However, the unpredictable responses of distinct algal species to cryopreservation agents and protocols have hampered the standardization of universal methods so far. The results presented in this study indicate that intrinsic biological factors (i.e.: cell morphology and phylogenetic origin) play a role in the definition of which culture age and cryoprotectant agent type and concentration should be used to achieve successful cryopreservation. Through the use of Central Composite Rotatable Design (CCRD) it was possible to define optimized protocols for cryopreserving three major morphotypic groups found among chlorophytes (i.e.: coenobium, coccoid or palmella forming strains). These optimized protocols were then validated upon fifteen strains from Sphaeropleales, Chlamydomonadales and Chlorelalles clades. Analysis reveals that the use of DMSO as the sole cryoprotectant provided the highest postfreezing cell viability recovery rates for 80% of the coccoid strains tested, while the combination of glycerol and PEG400 allowed efficient cryopreservation of 100% of the coenobium and palmella forming strains evaluated. In addition, results suggest that coenobium-forming strains have higher resistance to freeze/thawing when frozen at mid-log phase of growth, while coccoid and plamelloid strains present higher survival rates when cryopreserved at late-log phase. Such information is of valuable practical use since a simple visual inspection of algae strain's predominant morphotype can guide the choice of the most suitable cryopreservation protocol. MenosThe development of efficient algae cryopreservation methods is pivotal to the establishment of long-term culture collections as well as algae breeding and genetic modification programs. However, the unpredictable responses of distinct algal species to cryopreservation agents and protocols have hampered the standardization of universal methods so far. The results presented in this study indicate that intrinsic biological factors (i.e.: cell morphology and phylogenetic origin) play a role in the definition of which culture age and cryoprotectant agent type and concentration should be used to achieve successful cryopreservation. Through the use of Central Composite Rotatable Design (CCRD) it was possible to define optimized protocols for cryopreserving three major morphotypic groups found among chlorophytes (i.e.: coenobium, coccoid or palmella forming strains). These optimized protocols were then validated upon fifteen strains from Sphaeropleales, Chlamydomonadales and Chlorelalles clades. Analysis reveals that the use of DMSO as the sole cryoprotectant provided the highest postfreezing cell viability recovery rates for 80% of the coccoid strains tested, while the combination of glycerol and PEG400 allowed efficient cryopreservation of 100% of the coenobium and palmella forming strains evaluated. In addition, results suggest that coenobium-forming strains have higher resistance to freeze/thawing when frozen at mid-log phase of growth, while coccoid and plamelloid strains prese... Mostrar Tudo |
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Palavras-Chave: |
Central composite rotational design; Morphotype. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02383naa a2200217 a 4500
001 2107118
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
100 1 $aFERNANDES, M. S.
245 $aCustomized cryopreservation protocols for chlorophytes based on cell morphology.$h[electronic resource]
260 $c2019
520 $aThe development of efficient algae cryopreservation methods is pivotal to the establishment of long-term culture collections as well as algae breeding and genetic modification programs. However, the unpredictable responses of distinct algal species to cryopreservation agents and protocols have hampered the standardization of universal methods so far. The results presented in this study indicate that intrinsic biological factors (i.e.: cell morphology and phylogenetic origin) play a role in the definition of which culture age and cryoprotectant agent type and concentration should be used to achieve successful cryopreservation. Through the use of Central Composite Rotatable Design (CCRD) it was possible to define optimized protocols for cryopreserving three major morphotypic groups found among chlorophytes (i.e.: coenobium, coccoid or palmella forming strains). These optimized protocols were then validated upon fifteen strains from Sphaeropleales, Chlamydomonadales and Chlorelalles clades. Analysis reveals that the use of DMSO as the sole cryoprotectant provided the highest postfreezing cell viability recovery rates for 80% of the coccoid strains tested, while the combination of glycerol and PEG400 allowed efficient cryopreservation of 100% of the coenobium and palmella forming strains evaluated. In addition, results suggest that coenobium-forming strains have higher resistance to freeze/thawing when frozen at mid-log phase of growth, while coccoid and plamelloid strains present higher survival rates when cryopreserved at late-log phase. Such information is of valuable practical use since a simple visual inspection of algae strain's predominant morphotype can guide the choice of the most suitable cryopreservation protocol.
653 $aCentral composite rotational design
653 $aMorphotype
700 1 $aGARCIA, L. C.
700 1 $aNASCIMENTO, R. C.
700 1 $aSANTANA, H.
700 1 $aMORAIS, P. B.
700 1 $aCAPDEVILLE, G. de
700 1 $aBRASIL, B. dos S. A. F.
773 $tAlgal Research$gv. 38, 2019.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
20/08/2003 |
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Data da última atualização: |
08/06/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BARBOSA, F. R.; SOUZA, E. A, de; SILVA, C. S. B. da; SOUZA, F. A.; MOREIRA, W. A.; ALENCAR, J. A. de; HAJI, F. N. P. |
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Afiliação: |
FLAVIA RABELO BARBOSA, CPATSA; WELLINGTON ANTONIO MOREIRA, CPATSA; JOSE ADALBERTO DE ALENCAR, CPATSA; FRANCISCA NEMAURA PEDROSA HAJI, CPATSA. |
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Título: |
Biologia de Pleuroprucha asthenaria (Lepidoptera: Geometridae) em inflorescências de mangueira, como subsídio ao seu controle biológico. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 8., 2003, São Pedro, SP. Livro de resumos e programa oficial. Piracicaba: SEB, 2003. |
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Páginas: |
p. 191. |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho teve como objetivo determinar aspectos biológicos desta praga em inflorescências da mangueira. |
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Palavras-Chave: |
Inflorescência da mangueira; Insect. |
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Thesagro: |
Inseto; Lepidóptero; Manga; Mangifera Indica. |
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Thesaurus NAL: |
Mangoes. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/132737/1/26851.pdf
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Marc: |
LEADER 00990nam a2200277 a 4500
001 1148533
005 2022-06-08
008 2003 bl uuuu u00u1 u #d
100 1 $aBARBOSA, F. R.
245 $aBiologia de Pleuroprucha asthenaria (Lepidoptera$bGeometridae) em inflorescências de mangueira, como subsídio ao seu controle biológico.
260 $aIn: SIMPÓSIO DE CONTROLE BIOLÓGICO, 8., 2003, São Pedro, SP. Livro de resumos e programa oficial. Piracicaba: SEB$c2003
300 $ap. 191.
520 $aEste trabalho teve como objetivo determinar aspectos biológicos desta praga em inflorescências da mangueira.
650 $aMangoes
650 $aInseto
650 $aLepidóptero
650 $aManga
650 $aMangifera Indica
653 $aInflorescência da mangueira
653 $aInsect
700 1 $aSOUZA, E. A, de
700 1 $aSILVA, C. S. B. da
700 1 $aSOUZA, F. A.
700 1 $aMOREIRA, W. A.
700 1 $aALENCAR, J. A. de
700 1 $aHAJI, F. N. P.
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