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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
28/09/2010 |
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Data da última atualização: |
01/03/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TETZNER, T. A. D.; SARAIVA, N. Z.; OLIVEIRA, C. S.; NICIURA, S. C. M.; SOUZA M. M.; LIMA, M. R.; GARCIA J. M. |
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Afiliação: |
UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL. |
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Título: |
Effects of culture with ovalbumin in absence of fetal bovine serum and bovine serum albumin on in vitro production of cattle embryos. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, p. 197, 2010. |
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Idioma: |
Português |
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Conteúdo: |
Embryo quality is influenced by culture conditions, which affect IVM, IVF, and IVC rates. One of the most efficient ways to evaluate the embryonic quality of IVP blastocysts is by differential staining of inner cell mass (ICM) and trophoblast (TF). Bovine embryos of superior quality should present the total number of cells close to the number of cell cycles (Neuber et al. 2002 Theriogenology 57, 2193-2202). In this study, we analyzed the effects of fetal bovine serum (F) and bovine serum albumin (B) replacement for ovalbumin (O) on nuclear maturation, cortical granule migration, pronuclear development, blastocyst rates, and differential staining of ICM and TF in Day 7 blastocysts. The treatment groups were named as follows: the first letter is the protein source used for IVM, the second for IVF, and the third for IVC. When 2 protein sources were used in the same step, the plus symbol (+) was used. The oocytes were IVM in TCM-199, supplemented with the following: 10% F, or 4 mg mL-1 B, or 4 mg mL-1 O, and 1.0 Â?g mL-1 of FSH, 50 Â?g mL-1 of hCG, 1.0 Â?g mL-1 of estradiol, 0.2 mM sodium pyruvate, and 83.4 Â?g mL-1 of amikacin. IVF was accomplished in TALP-IVF medium, with 0.2 mM pyruvate, 83.4 Â?g mL-1 of amikacin, and 6 mg mL-1 B or O. IVC was in SOF, with F, B, or O. The control group (CONT) consisted of the treatment FBF + B. Pronuclear development was compared by the chi-square test, whereas the other results were analyzed by ANOVA followed by the Tukey test, using SAS at 5% significance level (SAS Institute Inc., Cary, NC, USA). For IVM, the treatments F, B, O, and B + O did not affect (P > 0.05) nuclear maturation (73.92 to 78.78%) and cortical granule migration rates (58.89 to 66.76%). Regarding pronuclear development, the treatment FO (76.67%) was similar (P > 0.05) to the control group (82.95%), which was superior (P < 0.05) to the treatments BB (56.98%), BO (39.02%), OB (37.36%), and OO (39.24%). Blastocyst rates in FBF (42.8%) and control (45.0%) groups were superior (P < 0.05) to treatment OOO (26.0%) but similar (P > 0.05) to FOF, BBB, BOB, and OBO (32.0 to 35.8%). The average of blastocyst ICM cells of the group OOO (16.79) was inferior (P < 0.05) to the other groups. However, the average of TF cells on blastocysts of the group OOO (38.25) was similar (P > 0.05) to the groups BBB (45.74) and BOB (45.60) and inferior (P < 0.05) to the groups CONT (57.59), FBF (54.41), FOF (56.74), and OBO (47.35). The total average cells in the blastocysts of the group OOO (56.04) was inferior (P < 0.05) to the groups CONT (84.86), FBF (78.96), FOF (81.32), BBB (68.11), BOB (69.55), and OBO (69.82). The total cell number in the treatments, with several sources of protein supplementation, varied from 56.04 to 84.86. Considering the evaluation interval, this average cell number was discreetly inferior to that expected for the chronological age of the blastocysts. We concluded that it is possible to produce bovine embryos in the absence of F and/or B, with the protein source O, although it reduced blastocyst rates when used in all 3 steps of embryo in vitro production and resulted in blastocysts of inferior quality. MenosEmbryo quality is influenced by culture conditions, which affect IVM, IVF, and IVC rates. One of the most efficient ways to evaluate the embryonic quality of IVP blastocysts is by differential staining of inner cell mass (ICM) and trophoblast (TF). Bovine embryos of superior quality should present the total number of cells close to the number of cell cycles (Neuber et al. 2002 Theriogenology 57, 2193-2202). In this study, we analyzed the effects of fetal bovine serum (F) and bovine serum albumin (B) replacement for ovalbumin (O) on nuclear maturation, cortical granule migration, pronuclear development, blastocyst rates, and differential staining of ICM and TF in Day 7 blastocysts. The treatment groups were named as follows: the first letter is the protein source used for IVM, the second for IVF, and the third for IVC. When 2 protein sources were used in the same step, the plus symbol (+) was used. The oocytes were IVM in TCM-199, supplemented with the following: 10% F, or 4 mg mL-1 B, or 4 mg mL-1 O, and 1.0 Â?g mL-1 of FSH, 50 Â?g mL-1 of hCG, 1.0 Â?g mL-1 of estradiol, 0.2 mM sodium pyruvate, and 83.4 Â?g mL-1 of amikacin. IVF was accomplished in TALP-IVF medium, with 0.2 mM pyruvate, 83.4 Â?g mL-1 of amikacin, and 6 mg mL-1 B or O. IVC was in SOF, with F, B, or O. The control group (CONT) consisted of the treatment FBF + B. Pronuclear development was compared by the chi-square test, whereas the other results were analyzed by ANOVA followed by the Tukey test, using SAS at ... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Culture; Embryo quality. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03978nam a2200217 a 4500
001 1863111
005 2011-03-01
008 2010 bl uuuu u00u1 u #d
100 1 $aTETZNER, T. A. D.
245 $aEffects of culture with ovalbumin in absence of fetal bovine serum and bovine serum albumin on in vitro production of cattle embryos.
260 $aIn: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, p. 197$c2010
520 $aEmbryo quality is influenced by culture conditions, which affect IVM, IVF, and IVC rates. One of the most efficient ways to evaluate the embryonic quality of IVP blastocysts is by differential staining of inner cell mass (ICM) and trophoblast (TF). Bovine embryos of superior quality should present the total number of cells close to the number of cell cycles (Neuber et al. 2002 Theriogenology 57, 2193-2202). In this study, we analyzed the effects of fetal bovine serum (F) and bovine serum albumin (B) replacement for ovalbumin (O) on nuclear maturation, cortical granule migration, pronuclear development, blastocyst rates, and differential staining of ICM and TF in Day 7 blastocysts. The treatment groups were named as follows: the first letter is the protein source used for IVM, the second for IVF, and the third for IVC. When 2 protein sources were used in the same step, the plus symbol (+) was used. The oocytes were IVM in TCM-199, supplemented with the following: 10% F, or 4 mg mL-1 B, or 4 mg mL-1 O, and 1.0 Â?g mL-1 of FSH, 50 Â?g mL-1 of hCG, 1.0 Â?g mL-1 of estradiol, 0.2 mM sodium pyruvate, and 83.4 Â?g mL-1 of amikacin. IVF was accomplished in TALP-IVF medium, with 0.2 mM pyruvate, 83.4 Â?g mL-1 of amikacin, and 6 mg mL-1 B or O. IVC was in SOF, with F, B, or O. The control group (CONT) consisted of the treatment FBF + B. Pronuclear development was compared by the chi-square test, whereas the other results were analyzed by ANOVA followed by the Tukey test, using SAS at 5% significance level (SAS Institute Inc., Cary, NC, USA). For IVM, the treatments F, B, O, and B + O did not affect (P > 0.05) nuclear maturation (73.92 to 78.78%) and cortical granule migration rates (58.89 to 66.76%). Regarding pronuclear development, the treatment FO (76.67%) was similar (P > 0.05) to the control group (82.95%), which was superior (P < 0.05) to the treatments BB (56.98%), BO (39.02%), OB (37.36%), and OO (39.24%). Blastocyst rates in FBF (42.8%) and control (45.0%) groups were superior (P < 0.05) to treatment OOO (26.0%) but similar (P > 0.05) to FOF, BBB, BOB, and OBO (32.0 to 35.8%). The average of blastocyst ICM cells of the group OOO (16.79) was inferior (P < 0.05) to the other groups. However, the average of TF cells on blastocysts of the group OOO (38.25) was similar (P > 0.05) to the groups BBB (45.74) and BOB (45.60) and inferior (P < 0.05) to the groups CONT (57.59), FBF (54.41), FOF (56.74), and OBO (47.35). The total average cells in the blastocysts of the group OOO (56.04) was inferior (P < 0.05) to the groups CONT (84.86), FBF (78.96), FOF (81.32), BBB (68.11), BOB (69.55), and OBO (69.82). The total cell number in the treatments, with several sources of protein supplementation, varied from 56.04 to 84.86. Considering the evaluation interval, this average cell number was discreetly inferior to that expected for the chronological age of the blastocysts. We concluded that it is possible to produce bovine embryos in the absence of F and/or B, with the protein source O, although it reduced blastocyst rates when used in all 3 steps of embryo in vitro production and resulted in blastocysts of inferior quality.
653 $aBovine
653 $aCulture
653 $aEmbryo quality
700 1 $aSARAIVA, N. Z.
700 1 $aOLIVEIRA, C. S.
700 1 $aNICIURA, S. C. M.
700 1 $aSOUZA M. M.
700 1 $aLIMA, M. R.
700 1 $aGARCIA J. M.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
05/09/2006 |
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Data da última atualização: |
18/05/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
VENDRUSCULO, L. G.; MARIN, F. R.; BARBARISI, B.; PILAU, F. G. |
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Afiliação: |
LAURIMAR GONCALVES VENDRUSCULO, CNPTIA; FÁBIO RICARDO MARIN, CNPTIA; BERNARD BARBARISI; FELIPE G. PILAU, Bolsista CNPq. |
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Título: |
Aplicação do text mining para incorporação de informações sócio-econômicas em sistemas objetivos de previsão de safra. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENGENHARIA AGRÍCOLA, 35., 2006, João Pessoa. Agroenergia e desenvolvimento tecnológico: anais. João Pessoa: SBEA, 2006. Não paginado. |
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Idioma: |
Português |
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Notas: |
CONBEA 2006. |
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Conteúdo: |
Vários estudos acadêmicos e esforços governamentais têm sido empreendidos para predizer, com confiança, a área plantada e a produtividade, no intuito de estimar oficialmente as safras agrícolas brasileiras. A estimativa oficial é baseada em levantamentos sistemáticos, por município, com informação colhida através de entrevistas em estabelecimentos rurais e outros setores organizados da sociedade. É importante, contudo, que outros fatores sejam considerados para a consolidação dos números regionais, estaduais e nacionais, especialmente, os fenômenos climáticos, condições para o manejo das lavouras, ocorrência generalizada de pragas e doenças. Sob esta ótica, o presente estudo apresenta a técnica de mineração de textos para incorporação de fatores sócio-econômicos no processo de previsão de safras. Estes fatores foram analisados no contexto de notícias jornalísticas por meio do software Eurekha, que possibilitou formar agrupamentos com índice de similaridades aceitáveis. |
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Palavras-Chave: |
Harvest forecast; Informação não estruturada; Mineração de texto; Non-structured information; Previsão de safras; Text mining. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160392/1/PL-Aplicacao-Vendrusculo-Conbea-2006.pdf
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Marc: |
LEADER 01889nam a2200229 a 4500
001 1006822
005 2022-05-18
008 2006 bl uuuu u00u1 u #d
100 1 $aVENDRUSCULO, L. G.
245 $aAplicação do text mining para incorporação de informações sócio-econômicas em sistemas objetivos de previsão de safra.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE ENGENHARIA AGRÍCOLA, 35., 2006, João Pessoa. Agroenergia e desenvolvimento tecnológico: anais. João Pessoa: SBEA, 2006. Não paginado.$c2006
500 $aCONBEA 2006.
520 $aVários estudos acadêmicos e esforços governamentais têm sido empreendidos para predizer, com confiança, a área plantada e a produtividade, no intuito de estimar oficialmente as safras agrícolas brasileiras. A estimativa oficial é baseada em levantamentos sistemáticos, por município, com informação colhida através de entrevistas em estabelecimentos rurais e outros setores organizados da sociedade. É importante, contudo, que outros fatores sejam considerados para a consolidação dos números regionais, estaduais e nacionais, especialmente, os fenômenos climáticos, condições para o manejo das lavouras, ocorrência generalizada de pragas e doenças. Sob esta ótica, o presente estudo apresenta a técnica de mineração de textos para incorporação de fatores sócio-econômicos no processo de previsão de safras. Estes fatores foram analisados no contexto de notícias jornalísticas por meio do software Eurekha, que possibilitou formar agrupamentos com índice de similaridades aceitáveis.
653 $aHarvest forecast
653 $aInformação não estruturada
653 $aMineração de texto
653 $aNon-structured information
653 $aPrevisão de safras
653 $aText mining
700 1 $aMARIN, F. R.
700 1 $aBARBARISI, B.
700 1 $aPILAU, F. G.
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