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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/10/1993 |
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Data da última atualização: |
05/10/1993 |
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Autoria: |
GARCIA, M. L.; ARRESE, E. L.; GRAU, O.; SARACHU, A. N. |
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Afiliação: |
Catedra de Quimica Biologica II, Facultad de Ciencias Exactas, U. N.,La Plata 47 y 115, 1900 La Plata, Argentina. |
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Título: |
Citrus Psorosic Disease Agent Behaves as a two Componet ss RNA Virus. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Riverside, California: International Organization of Citrus Virologists, 1991. |
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Páginas: |
337-344 |
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Idioma: |
Inglês |
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Notas: |
Conference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings... |
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Conteúdo: |
Citrus seedings were graft-inoculated with psorosis (isolate 90-1-1;Concordia, Argentina).When symptomatic young leaves were ground, homogeniized and centrifuged at low speed, infectivity (local lesions on Chenopodium quinoa) remained in the supernatant.Polyethylene glycol precipitation and high-speed centrifugation yielded infective pellets. Infectivity was abolished by RNase treatment, even in 0.3 M NaCI,indicating that ss RNA (s) is essential for biological activity. Protein(s) is also required for infectivity, since it was sensitivy to phenol extraction and proteinase K treatment. When high speed pellts were subjected to sucrose density gradient centrifugation, none of the single fractios accounted for loaded infectivity, but some of the two-fold combinations were much more highy infections than expected from the contribution of single fraction. Therefore two components activate each other for infectivity.Protein analysis of slow- and fast- sedimenting components sugeested that a protein of about 50 KD MW is associated with infectivity. We interprete these and other results as indicating that a bipartit virus is associated with psorosis disease. The virus has ss RNA as its genetic material and a 50 Kd polypeptide as one of its structural protein(s). |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01887naa a2200181 a 4500
001 1644269
005 1993-10-05
008 1991 bl uuuu u00u1 u #d
100 1 $aGARCIA, M. L.
245 $aCitrus Psorosic Disease Agent Behaves as a two Componet ss RNA Virus.
260 $c1991
300 $a337-344
500 $aConference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings...
520 $aCitrus seedings were graft-inoculated with psorosis (isolate 90-1-1;Concordia, Argentina).When symptomatic young leaves were ground, homogeniized and centrifuged at low speed, infectivity (local lesions on Chenopodium quinoa) remained in the supernatant.Polyethylene glycol precipitation and high-speed centrifugation yielded infective pellets. Infectivity was abolished by RNase treatment, even in 0.3 M NaCI,indicating that ss RNA (s) is essential for biological activity. Protein(s) is also required for infectivity, since it was sensitivy to phenol extraction and proteinase K treatment. When high speed pellts were subjected to sucrose density gradient centrifugation, none of the single fractios accounted for loaded infectivity, but some of the two-fold combinations were much more highy infections than expected from the contribution of single fraction. Therefore two components activate each other for infectivity.Protein analysis of slow- and fast- sedimenting components sugeested that a protein of about 50 KD MW is associated with infectivity. We interprete these and other results as indicating that a bipartit virus is associated with psorosis disease. The virus has ss RNA as its genetic material and a 50 Kd polypeptide as one of its structural protein(s).
700 1 $aARRESE, E. L.
700 1 $aGRAU, O.
700 1 $aSARACHU, A. N.
773 $tRiverside, California: International Organization of Citrus Virologists, 1991.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
13/02/2012 |
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Data da última atualização: |
13/02/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BORGUINI, M. G.; EVANGELISTA, R. M.; SALATA, A. da C.; BORGUINI, R. G. |
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Afiliação: |
MILENA GALHARDO BORGUINI, UNESP; REGINA MARTA EVANGELISTA, UNESP; ARIANE DA CUNHA SALATA, UNESP; RENATA GALHARDO BORGUINI, CTAA. |
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Título: |
Conservação pós-colheita de carambolas tratadas com ácido ascórbico, cloreto de cálcio e EDTA de cálcio associado ao uso de filme plástico. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE PÓS-COLHEITA DE FRUTAS, HORTALIÇAS E FLORES, 3., 2011, Nova Friburgo. Anais... Rio de Janeiro: Embrapa Agroindústria de Alimentos, 2011. 1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Açúcares redutores; Averrhoa carambola L; Policloreto de vinila; Polietileno de baixa densidade; Taxa respiratória. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53979/1/2011-096.pdf
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Marc: |
LEADER 00812nam a2200193 a 4500
001 1915247
005 2012-02-13
008 2011 bl uuuu u00u1 u #d
100 1 $aBORGUINI, M. G.
245 $aConservação pós-colheita de carambolas tratadas com ácido ascórbico, cloreto de cálcio e EDTA de cálcio associado ao uso de filme plástico.
260 $aIn: SIMPÓSIO BRASILEIRO DE PÓS-COLHEITA DE FRUTAS, HORTALIÇAS E FLORES, 3., 2011, Nova Friburgo. Anais... Rio de Janeiro: Embrapa Agroindústria de Alimentos, 2011. 1 CD-ROM.$c2011
653 $aAçúcares redutores
653 $aAverrhoa carambola L
653 $aPolicloreto de vinila
653 $aPolietileno de baixa densidade
653 $aTaxa respiratória
700 1 $aEVANGELISTA, R. M.
700 1 $aSALATA, A. da C.
700 1 $aBORGUINI, R. G.
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Embrapa Agroindústria de Alimentos (CTAA) |
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