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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/08/2005 |
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Data da última atualização: |
11/08/2005 |
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Autoria: |
GAIOTTO, F. A.; BRAMUCCI, M.; GRATTAPAGLIA, D. |
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Título: |
Estimation of outcrossing rate in a breeding population of Eucalyptus urophylla with dominant RAPD and AFLP markers. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Theoretical and Applied Genetics, v. 95, n. 5/6, p. 842-849, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Eucalyptus breeding is typically conducted by selection in open-pollinated progenies. As mating is controlled only on the female side of the cross, knowledge of outcrossing versus selfing rates is essential for maintaining adequate levels of genetic variability for continuous gains. Outcrossing rate in an open-pollinated breeding population of Eucalyptus urophylla was estimated by two PCR-based dominant marker technologies, RAPD and AFLP, using 11 open-pollinated progeny arrays of 24 individuals. Estimated outcrossing rates indicate predominant outcrossing and suggest maintenance of adequate genetic variability within families. The multilocus outcrossing rate (tm) estimated from RAPD markers (0.93 +or- 0.027), although in the same range, was higher ( alpha > 0.01) than the estimate based on AFLP (0.89 +or- 0.033). Both estimates were of similar magnitude to those estimated for natural populations using isozymes. The estimated Wright's fixation index was lower than expected based on tm possibly resulting from selection against selfed seedlings when sampling plants for the study. An empirical analysis suggests that 18 is the minimum number of dominant marker loci necessary to achieve robust estimates of tm. This study demonstrates the usefulness of dominant markers, both RAPD and AFLP, for estimating the outcrossing rate in breeding and natural populations of forest trees. It is anticipated that there will be an increasing use of such PCR-based technologies in mating-system studies, in view of their high throughput and universality of the reagents, particularly for species where isozyme systems have not yet been optimized. MenosEucalyptus breeding is typically conducted by selection in open-pollinated progenies. As mating is controlled only on the female side of the cross, knowledge of outcrossing versus selfing rates is essential for maintaining adequate levels of genetic variability for continuous gains. Outcrossing rate in an open-pollinated breeding population of Eucalyptus urophylla was estimated by two PCR-based dominant marker technologies, RAPD and AFLP, using 11 open-pollinated progeny arrays of 24 individuals. Estimated outcrossing rates indicate predominant outcrossing and suggest maintenance of adequate genetic variability within families. The multilocus outcrossing rate (tm) estimated from RAPD markers (0.93 +or- 0.027), although in the same range, was higher ( alpha > 0.01) than the estimate based on AFLP (0.89 +or- 0.033). Both estimates were of similar magnitude to those estimated for natural populations using isozymes. The estimated Wright's fixation index was lower than expected based on tm possibly resulting from selection against selfed seedlings when sampling plants for the study. An empirical analysis suggests that 18 is the minimum number of dominant marker loci necessary to achieve robust estimates of tm. This study demonstrates the usefulness of dominant markers, both RAPD and AFLP, for estimating the outcrossing rate in breeding and natural populations of forest trees. It is anticipated that there will be an increasing use of such PCR-based technologies in mating-system st... Mostrar Tudo |
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Palavras-Chave: |
AFLP; RAPD. |
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Thesagro: |
Biotecnologia; Eucalipto. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02217naa a2200193 a 4500
001 1171700
005 2005-08-11
008 1997 bl uuuu u00u1 u #d
100 1 $aGAIOTTO, F. A.
245 $aEstimation of outcrossing rate in a breeding population of Eucalyptus urophylla with dominant RAPD and AFLP markers.
260 $c1997
520 $aEucalyptus breeding is typically conducted by selection in open-pollinated progenies. As mating is controlled only on the female side of the cross, knowledge of outcrossing versus selfing rates is essential for maintaining adequate levels of genetic variability for continuous gains. Outcrossing rate in an open-pollinated breeding population of Eucalyptus urophylla was estimated by two PCR-based dominant marker technologies, RAPD and AFLP, using 11 open-pollinated progeny arrays of 24 individuals. Estimated outcrossing rates indicate predominant outcrossing and suggest maintenance of adequate genetic variability within families. The multilocus outcrossing rate (tm) estimated from RAPD markers (0.93 +or- 0.027), although in the same range, was higher ( alpha > 0.01) than the estimate based on AFLP (0.89 +or- 0.033). Both estimates were of similar magnitude to those estimated for natural populations using isozymes. The estimated Wright's fixation index was lower than expected based on tm possibly resulting from selection against selfed seedlings when sampling plants for the study. An empirical analysis suggests that 18 is the minimum number of dominant marker loci necessary to achieve robust estimates of tm. This study demonstrates the usefulness of dominant markers, both RAPD and AFLP, for estimating the outcrossing rate in breeding and natural populations of forest trees. It is anticipated that there will be an increasing use of such PCR-based technologies in mating-system studies, in view of their high throughput and universality of the reagents, particularly for species where isozyme systems have not yet been optimized.
650 $aBiotecnologia
650 $aEucalipto
653 $aAFLP
653 $aRAPD
700 1 $aBRAMUCCI, M.
700 1 $aGRATTAPAGLIA, D.
773 $tTheoretical and Applied Genetics$gv. 95, n. 5/6, p. 842-849, 1997.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
05/04/2016 |
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Data da última atualização: |
12/04/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA FILHO, J. L. da; SUASSUNA, N. D.; MORELLO, C. de L.; PEDROSA, M. B.; LOPES, G. D.; BEZERRA, W.; FARIAS, F. J. C. |
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Afiliação: |
JOAO LUIS DA SILVA FILHO, CNPA; NELSON DIAS SUASSUNA, CNPA; CAMILO DE LELIS MORELLO, CNPA; MURILO BARROS PEDROSA, FUNDAÇÃO BAHIA; GEDEON DIAS LOPES, CNPA; WASHINGTON BEZERRA, CNPA; FRANCISCO JOSE CORREIA FARIAS, CNPA. |
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Título: |
Ensaios de valor de cultivo e uso de linhagens transgênicas do Programa de Melhoramento da Embrapa Algodão no Estado de Goiás. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DO ALGODÃO, 10., 2015, Foz do Iguaçu. Resumos. Brasília, DF: ABRAPA, 2015. |
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Idioma: |
Português |
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Conteúdo: |
O Ministério da Agricultura, Pecuária e Abastecimento exige para o lançamento de novas linhagens como cultivares a realização de Ensaios de Valor de Cultivo e Uso de modo que linhagens tenham valor agronômico comprovado em condições de cultivo, em várias locais e safras. O presente trabalho teve por objetivo avaliar o ensaio VCU de linhagens transgênicas do programa de melhoramento da Embrapa Algodão frente a cultivares comerciais. |
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Palavras-Chave: |
MON 88913; VCU. |
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Thesagro: |
Gossypium hirsutum; Linhagem. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142044/1/29-ENSAIOS-DE-VALOR-DE-CULTIVO-CAMILO.pdf
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Marc: |
LEADER 01203nam a2200229 a 4500
001 2042656
005 2024-04-12
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA FILHO, J. L. da
245 $aEnsaios de valor de cultivo e uso de linhagens transgênicas do Programa de Melhoramento da Embrapa Algodão no Estado de Goiás.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DO ALGODÃO, 10., 2015, Foz do Iguaçu. Resumos. Brasília, DF: ABRAPA$c2015
520 $aO Ministério da Agricultura, Pecuária e Abastecimento exige para o lançamento de novas linhagens como cultivares a realização de Ensaios de Valor de Cultivo e Uso de modo que linhagens tenham valor agronômico comprovado em condições de cultivo, em várias locais e safras. O presente trabalho teve por objetivo avaliar o ensaio VCU de linhagens transgênicas do programa de melhoramento da Embrapa Algodão frente a cultivares comerciais.
650 $aGossypium hirsutum
650 $aLinhagem
653 $aMON 88913
653 $aVCU
700 1 $aSUASSUNA, N. D.
700 1 $aMORELLO, C. de L.
700 1 $aPEDROSA, M. B.
700 1 $aLOPES, G. D.
700 1 $aBEZERRA, W.
700 1 $aFARIAS, F. J. C.
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