Data/hora: 18/06/2024 13:57:12

Biblioteca(s): | Embrapa Café.
Data corrente: | 13/04/2011
Data da última atualização: | 13/04/2011
Autoria: | EIRA, M. T. S. da; RIBEIRO, V. S.; FUZUOLI, L. C.; GUERREIRO FILHO, O.; WALTERS, C.
Afiliação: | MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; IAC; IAC; NCGRP/USDA.
Título: | Coffee seed cryopreservation: current research progress.
Ano de publicação: | 2007
Fonte/Imprenta: | In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.
Idioma: | Inglês
Conteúdo: | Viability of seeds stored in genebanks must be maintained for several years or even centuries. Because of the difficulties in storing the seeds, coffee germplasm is maintained in field collections, presenting significant problems, such as land and labor costs and susceptibility to environmental hazards and pathogens. Storage of Coffea species in ex situ genebanks may help to preserve the threatened diversity of this important genus. Since 1976 the International Plant Genetic Resources Institute has considered coffee as a high priority for genetic conservation. For non-orthodox seed species, cryopreservation is the only technique available for long-term germplasm conservation. The genebank of Embrapa Genetic Resources and Biotechnology, in Brazil has now established a program to cryopreserve genetic resources of Coffea. The protocol was first determined for C. arabica and C. racemosa. Seeds were first dried to 0.20 g/g (in equilibrium with 78-80% RH). Sufficiently rapid cooling and warming was achieved in hermetically-sealed foil-laminate bags containing 10-11 g (or 50 seeds) by plunging bags directly into liquid nitrogen ? LN containers and placing bags removed from LN directly into a 40 °C bath. Successfully cryopreserved C. arabica and C. racemosa seeds showed minimal viability loss after two-year storage in liquid nitrogen. The same protocol is being adapted to other species of Coffea.
Palavras-Chave: | Coffee germplasm; Genebanks.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/32596/1/Coffee-Seed-Cryopreservation.pdf
Registro original: | Embrapa Café (CNPCa)
Exemplares: | CNPCa - (195-1) - UPC - AA - DD -  -  -  - DIS