Data/hora: 15/06/2024 00:57:14

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 06/04/2004
Data da última atualização: | 06/04/2004
Autoria: | CARVALHO, L. J. C. B.; SCHAAL, B. A.; FUKUDA, W. M. G.
Afiliação: | Embrapa Mandioca e Fruticultura, Cruz das Almas.
Título: | Phenetic relationships and genetic diversity among cassava (Manihot esculenta Crantz) land races revealed by PCR-based markers.
Ano de publicação: | 1998
Fonte/Imprenta: | Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p.14, nov., 1998., Suplemento.
Idioma: | Inglês
Conteúdo: | Abstract: Two PCR-based molecular genetic markers were used to examine the distribution of genetic divercity among cassava accessions representing seven habitats in Brazil. RAPD, and Microsatellite-primed PCR markers, together with phenetic analysis and nucleotide diversity and divergence were used. The level of band polymorphism among cassava accessions varies with the kind of marker, being high in RAPD than in microsatellite-primed PCR. The distinctness of clusters identified by UPGMA-derived phenogram was higher for the microsatellite-primed PCR than for RAPD analysis as indicated by the matrix correlation and consensus tree analysis. This observation was reflected on the grouping pattern differentiation obtained by the two markers. The RAPD polymorphism showed resolution to from distinct groups of individuals for accessions from Cerrados (CE) and Caatinga (CA), but not from other regions. While the microsatellite-primed PCR formed distinct groups of individuals for accessions from Humid Tropics (HT), Sub Tropics (ST), Cerrados (CE), Semi Arid (SA) and Littoral (LI). The polymorphism of both techniques did not show resolution to resolve individuals for geographic regions from Wide adaptation clone. Overall, the nucleotide diversity (pi values) were 0.3% higher in RAPD markers than in microsatellite-primed PCR. This indicate that the two markers hits different regions in the genome of cassava.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (20914-1) - ADD - AP - -- - 633.68205 -  -  - DIS