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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
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Data corrente: |
18/12/2020 |
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Data da última atualização: |
26/02/2021 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; MOLINARI, M. D. C.; BARBOSA, D. de A.; MOLINARI, H. B. C.; MERTZ-HENNING, L. M.; NEUMAIER, N.; FARIAS, J. R. B.; NAKASHIMA, K.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L. |
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Afiliação: |
Renata Fuganti-Pagliarini, National Council for Scientific and Technological Development (CNPq).; SILVANA REGINA ROCKENBACH MARIN, CNPSO; Mayla Daiane Côrrea Molinari, Universidade Estadual de Londrina, UEL, Londrina, PR.; Daniel de Amorim Barbosa, Universidade Estadual de Londrina, UEL, Londrina, PR.; HUGO BRUNO CORREA MOLINARI, CNPAE; LILIANE MARCIA MERTZ HENNING, CNPSO; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; Kazuo Nakashima, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan; Kazuko Yamaguchi-Shinozaki, Research Institute for Agricultural and Life Sciences, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Drought-tolerant soybean development: evaluation of GM lines under greenhouse and field conditions. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
In: NAKASHIMA, K.; URAO, Takeshi. (Ed.). Development of biotechnologies and biotech crops for stable food production under adverse environments and changing climate conditions. Tsukuba: JIRCAS, 2020. p. 57-88. (JIRCAS Working Report, 91). |
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Idioma: |
Inglês |
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Conteúdo: |
Drought is one of the greatest sources of environmental stress that has resulted in both economic and yield losses in many soybean-producing regions. The use of biotechnological tools aimed to produce plants with increased drought tolerance and productivity is the result of major investments in scientific and technological research. During the last decades, transcription factors (TFs) and key genes of important drought-responsive pathways have been used to develop genetically modified (GM) plants with increased tolerance to abiotic stress. To develop soybean lines with improved drought tolerance, genes encoding dehydration-responsive element binding protein (DREB) TFs and ABA-responsive element-binding proteins (AREB) as well as the GolS and NCED genes that encode the enzyme galactinol synthase (GolS, EC 2.4.1.123) of the raffinose family of oligosaccharides (RFOs) and the key enzyme in abscisic acid (ABA) biosynthesis 9-cisepoxycarotenoid dioxygenase (NCED, EC 1.13.11.51), respectively, were successfully introduced in soybean plants. After transformation, it was imperative to characterize the resultant drought tolerance of the GM lines. Thus, the soybean GM lines containing genetic constructions to overexpress AtDREB1A, GmDREB1A, AtDREB2A, AtAREB1, AtGolS2, and AtNCED3 were phenotyped based on molecular and agronomical traits, growth parameters, and survival rates under water deficit conditions in experiments carried out under greenhouse and field conditions for more than one crop season. All obtained results were compiled and are presented in this report. Overall, the GM lines are promising and show improved drought tolerance as diverse defense mechanisms aimed at surviving periods of water scarcity were targeted while retaining the productivity of the crop. These outcomes highlight soybean drought-tolerance pathways and indicate that the use of biotechnological tools in agricultural research can help producers minimize both yield and financial losses during drought-stricken crop seasons. MenosDrought is one of the greatest sources of environmental stress that has resulted in both economic and yield losses in many soybean-producing regions. The use of biotechnological tools aimed to produce plants with increased drought tolerance and productivity is the result of major investments in scientific and technological research. During the last decades, transcription factors (TFs) and key genes of important drought-responsive pathways have been used to develop genetically modified (GM) plants with increased tolerance to abiotic stress. To develop soybean lines with improved drought tolerance, genes encoding dehydration-responsive element binding protein (DREB) TFs and ABA-responsive element-binding proteins (AREB) as well as the GolS and NCED genes that encode the enzyme galactinol synthase (GolS, EC 2.4.1.123) of the raffinose family of oligosaccharides (RFOs) and the key enzyme in abscisic acid (ABA) biosynthesis 9-cisepoxycarotenoid dioxygenase (NCED, EC 1.13.11.51), respectively, were successfully introduced in soybean plants. After transformation, it was imperative to characterize the resultant drought tolerance of the GM lines. Thus, the soybean GM lines containing genetic constructions to overexpress AtDREB1A, GmDREB1A, AtDREB2A, AtAREB1, AtGolS2, and AtNCED3 were phenotyped based on molecular and agronomical traits, growth parameters, and survival rates under water deficit conditions in experiments carried out under greenhouse and field conditions for more than o... Mostrar Tudo |
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Palavras-Chave: |
9-cisepoxycarotenoid dioxygenase; ABA; AREB; DREB; Galactinol synthase. |
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Thesagro: |
Deficiência Hídrica; Glycine Max; Produção; Soja. |
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Thesaurus Nal: |
Soil water deficit; Transcription factors; Yields. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219437/1/Drought-tolerant-soybean-development-evaluation-of-GM-lines-under.pdf
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Marc: |
LEADER 03314naa a2200385 a 4500
001 2128419
005 2021-02-26
008 2020 bl uuuu u00u1 u #d
100 1 $aFUGANTI-PAGLIARINI, R.
245 $aDrought-tolerant soybean development$bevaluation of GM lines under greenhouse and field conditions.$h[electronic resource]
260 $c2020
520 $aDrought is one of the greatest sources of environmental stress that has resulted in both economic and yield losses in many soybean-producing regions. The use of biotechnological tools aimed to produce plants with increased drought tolerance and productivity is the result of major investments in scientific and technological research. During the last decades, transcription factors (TFs) and key genes of important drought-responsive pathways have been used to develop genetically modified (GM) plants with increased tolerance to abiotic stress. To develop soybean lines with improved drought tolerance, genes encoding dehydration-responsive element binding protein (DREB) TFs and ABA-responsive element-binding proteins (AREB) as well as the GolS and NCED genes that encode the enzyme galactinol synthase (GolS, EC 2.4.1.123) of the raffinose family of oligosaccharides (RFOs) and the key enzyme in abscisic acid (ABA) biosynthesis 9-cisepoxycarotenoid dioxygenase (NCED, EC 1.13.11.51), respectively, were successfully introduced in soybean plants. After transformation, it was imperative to characterize the resultant drought tolerance of the GM lines. Thus, the soybean GM lines containing genetic constructions to overexpress AtDREB1A, GmDREB1A, AtDREB2A, AtAREB1, AtGolS2, and AtNCED3 were phenotyped based on molecular and agronomical traits, growth parameters, and survival rates under water deficit conditions in experiments carried out under greenhouse and field conditions for more than one crop season. All obtained results were compiled and are presented in this report. Overall, the GM lines are promising and show improved drought tolerance as diverse defense mechanisms aimed at surviving periods of water scarcity were targeted while retaining the productivity of the crop. These outcomes highlight soybean drought-tolerance pathways and indicate that the use of biotechnological tools in agricultural research can help producers minimize both yield and financial losses during drought-stricken crop seasons.
650 $aSoil water deficit
650 $aTranscription factors
650 $aYields
650 $aDeficiência Hídrica
650 $aGlycine Max
650 $aProdução
650 $aSoja
653 $a9-cisepoxycarotenoid dioxygenase
653 $aABA
653 $aAREB
653 $aDREB
653 $aGalactinol synthase
700 1 $aMARIN, S. R. R.
700 1 $aMOLINARI, M. D. C.
700 1 $aBARBOSA, D. de A.
700 1 $aMOLINARI, H. B. C.
700 1 $aMERTZ-HENNING, L. M.
700 1 $aNEUMAIER, N.
700 1 $aFARIAS, J. R. B.
700 1 $aNAKASHIMA, K.
700 1 $aYAMAGUCHI-SHINOZAKI, K.
700 1 $aNEPOMUCENO, A. L.
773 $tIn: NAKASHIMA, K.; URAO, Takeshi. (Ed.). Development of biotechnologies and biotech crops for stable food production under adverse environments and changing climate conditions. Tsukuba: JIRCAS, 2020. p. 57-88. (JIRCAS Working Report, 91).
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 64 | |
| 4. |  | RAKOCEVIC, M.; MULLER, M.; MATSUNAGA, F. T.; NEUMAIER, N.; FARIAS, J. R. B.; NEPOMUCENO, A. L.; FUGANTI-PAGLIARINI, R. Daily heliotropic movements assist gas exchange and productive responses in DREB1A soybean plants under drought stress in the greenhouse. The Plant Journal, v. 96, n. 4, p. 801-814, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | MOLINARI, M. D. C.; BARBOSA, D. A.; MARIN, S. R. R.; ANDREATTA, E. C.; FUGANTI-PAGLIARINI, R.; MERTZ-HENNING, L. M.; HARMON, F.; NEPOMUCENO, A. L. Análise in sílico de splicing alternativo em Arabidopsis thaliana DBR1 mutante. In: CONGRESO DE LA SOJA DEL MERCOSUR, 7.; A TODA SOJA, 2019, Rosario. Reformular la Soja para Impulsar una Cadena de Conocimiento: [anais]. Rosario: ACSOJA, 2019. MERCOSOJA, 2019.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
|  |
| 7. | | FUGANTI-PAGLIARINI, R.; RODRIGUES, F. A.; ENGELS, C.; MARIN, S. R. R.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. Análise do transcriptoma e caracterização de promotores estresse-induzidos em soja submetida ao déficit hídrico. In: CONGRESSO BRASILEIRO DE SOJA, 7.; MERCOSOJA, 2015, Florianópolis. Tecnologia e mercado global: perspectivas para soja: anais. Londrina: Embrapa Soja, 2015. 3 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
|  |
| 8. | | BARBOSA, D. de A.; MOLINARI, M. D. C.; FUGANTI-PAGLIARINI, R.; KAFER, J. M.; MARIN, D. C.; MARIN, S. R. R.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L. Caracterização in silico de genes drip em Glycine max e Glycine soja. In: CONGRESSO BRASILEIRO DE SOJA, 9., 2022, Foz do iguaçu, PR. Desafios para a produtividade sustentável no Mercosul: resumos. Brasília, DF: Embrapa, 2022. Regina Maria Villas Bôas de Campos Leite, Adeney de Freitas Bueno, editores técnicos. resumo 144. p. 164.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | LEITE, J. P.; BARBOSA, E. G. G; MARIN, S. R. R.; MARINHO, J. P.; FUGANTI-PAGLIARINI, R.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.; DESIDÉRIO, J. A. Caracterização de plantas transgênicas de soja obtidas com a forma constitutiva do fator de transcrição AREB1. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 14, res. 6.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 10. | | LEITE, J. P.; BARBOSA, E. G. G; MARIN, S. R. R.; MARINHO, J. P.; FUGANTI-PAGLIARINI, R.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.; DESIDÉRIO, J. A. Caracterização de plantas transgênicas de soja obtidas com a forma constitutiva do fator de transcrição AREB1. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. 5 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | DIAS, A. S.; MARIN, S. R. R.; FUGANTI-PAGLIARINI, R.; SILVEIRA, C. A.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; DIAS, W. P. Resposta de plantas de soja transformadas com fator de transcrição aba dependente ao nematoide de cisto Heterodera glycines. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 10., 2015, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2015. p. 21-26.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 12. | | BARBOSA. D. A.; MOLINARI, M. D. C.; FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; CARANHATO, A. L. H.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L. Seleção de plantas de soja geneticamente modificadas para resistência ao herbicida glufosinato de amônio. In: CONGRESSO BRASILEIRO DE SOJA, 8., 2018, Goiânia. Inovação, tecnologias digitais e sustentabilidade da soja: anais. Brasília, DF: Embrapa, 2018. p. 672-674.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 13. | | GOMES, J. M.; NAKAYAMA, T. J.; REIS, R. R.; RODRIGUES, F. A.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; MOLINARI, H. B. C.; HARMON, F.; NEPOMUCENO, A. L. Gene expression and functional analysis of soybean genes with diurnal oscillation during drought stress. In: PLANT & ANIMAL GENOME XXIII, 2015, San Diego. PO 203| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 14. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; HARMONAND, F. G.; NEPOMUCENO, A. L. Gene expression networks and cis-elements combinatorial models in soybean: circadian clock and drought responses. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 45-46.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 15. | | MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; BASSO, M. F.; HENNING, L. M. M.; FUGANTI-PAGLIARINI, R.; HARMON, F. G.; NEPOMUCENO, A. L. Functional characterization of a putative Glycine max ELF4 in transgenic Arabidopsis and its role during flowering control. Frontiers in Plant Science, v. 8, artigo 618, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 16. | | MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; BASSO, M. F.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; HARMON, F. G.; NEPOMUCENO, A. L. Functional characterization of a putative Glycine max ELF4 in transgenic arabidopsis and its role during flowering control. Frontiers in Plant Science, v. 8, artigo 618, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 17. | | MOLINARI, M. D. C.; BARBOSA, D. A.; FUGANTI-PAGLIARINI, R.; ANDREATA, E. C.; CARANHATO, A. L. H.; MARIN, S. R. R.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L. Influência do tamanho de reads e presença de duplicatas não naturais em bibliotecas de RNA-Seq de soja sob deficit hídrico. In: CONGRESSO BRASILEIRO DE SOJA, 8., 2018, Goiânia. Inovação, tecnologias digitais e sustentabilidade da soja: anais. Brasília, DF: Embrapa, 2018. p. 688-690.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 18. | | FUGANTI-PAGLIARINI, R.; MOLINARI, M. D. C.; BARBOSA, D. A.; CARANHATO, A. L. H.; MARIN, S. R. R.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L. Correlação entre a porcentagem de alinhamentos únicos em bibliotecas de RNAseq de soja sob deficit hídrico com diferentes tamanhos de fragmento. In: CONGRESSO BRASILEIRO DE SOJA, 8., 2018, Goiânia. Inovação, tecnologias digitais e sustentabilidade da soja: anais. Brasília, DF: Embrapa, 2018. p. 685-687.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 19. | | RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; LOBO, F. P.; HARMON, F. G.; NEPAMUCENO, A. L. Daytime soybean transcriptome fluctuations during water deficit stress. BMC Genomics, v. 16, n. 505, p. 2-19, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 20. | | RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; LOBO, F. P.; HARMON, F. G.; NEPOMUCENO, A. L. Daytime soybean transcriptome fluctuations during water deficit stress. BMC Genomics, v. 16, n. 505, 2015. 19 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 64 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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