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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/02/2009 |
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Data da última atualização: |
10/02/2009 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, P. K.; JORDÃO, B. Q.; YAMANAKA, N.; FARIAS, J. R. B.; BENEVENTI, M. A.; BINNECK, E.; FUGANTI, R.; STOLF, R.; NEPOMUCENO, A. L. |
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Afiliação: |
Polyana K. Martins, UEL; Berenice Q. Jordão, UEL; Naoki Yamanaka, JIRCAS; José Renato Bouças Farias. CNPSo; Magda Aparecida Beneventi, UEL; Eliseu Binneck, CNPSo; Renata Fuganti, UEL; Renata Stolf, UEL; Alexandre Lima Nepomuceno, CNPSo. |
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Título: |
Differential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 31, n. 2, p. 512-521, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Drought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water- stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the Arabidopsis thaliana phosphatidylinositol transfer protein PITP and the auxin-independent growth regulator 1 (axi 1). The hydroponic experiments showed that after 100 min outside the nutrient solution photosynthesis completely stopped, stomata closed and leaf temperature rose. Both stress induction treatments produced significant decrease in the mitotic indices of root cells. Axi 1, PITP and bHLH were not only differentially expressed during dehydration in the hydroponics experiments but also during induced drought in the pot experiments. Although, there were differences between the two sets of experiments in the time at which up or down regulation occurred, the expression pattern of all three transcripts was related. Similar gene expression and cytological analysis results occurred in both systems, suggesting that hydroponics could be used to simulate drought detection by roots growing in soil and thus facilitate rapid and easy root sampling. MenosDrought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water- stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the Arabidopsis thaliana phosphatidylinositol transfer protein PITP and the auxin-independent growth regulator 1 (axi 1). The hydroponic experiments showed that after 100 min outside the nutrient solution photosynthesis completely stopped, stomata closed and leaf temperature rose. Both stress induction treatments produced significant decrease in the mitotic indices of root cells. Axi 1, PITP and bHLH were not only differentially expressed during dehydration in the hydroponics experiments but also during induced drought in the pot experiments. Although, there were differences between the two sets of experiments in the time at which up or down regulation occurred, the expression pattern of all three transcripts was related. Simila... Mostrar Tudo |
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Categoria do assunto: |
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URL: |
https://www.scielo.br/pdf/gmb/v31n2/a19v31n2.pdf
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Marc: |
LEADER 02469naa a2200217 a 4500
001 1471496
005 2009-02-10
008 2008 bl --- 0-- u #d
100 1 $aMARTINS, P. K.
245 $aDifferential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems.
260 $c2008
520 $aDrought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water- stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the Arabidopsis thaliana phosphatidylinositol transfer protein PITP and the auxin-independent growth regulator 1 (axi 1). The hydroponic experiments showed that after 100 min outside the nutrient solution photosynthesis completely stopped, stomata closed and leaf temperature rose. Both stress induction treatments produced significant decrease in the mitotic indices of root cells. Axi 1, PITP and bHLH were not only differentially expressed during dehydration in the hydroponics experiments but also during induced drought in the pot experiments. Although, there were differences between the two sets of experiments in the time at which up or down regulation occurred, the expression pattern of all three transcripts was related. Similar gene expression and cytological analysis results occurred in both systems, suggesting that hydroponics could be used to simulate drought detection by roots growing in soil and thus facilitate rapid and easy root sampling.
700 1 $aJORDÃO, B. Q.
700 1 $aYAMANAKA, N.
700 1 $aFARIAS, J. R. B.
700 1 $aBENEVENTI, M. A.
700 1 $aBINNECK, E.
700 1 $aFUGANTI, R.
700 1 $aSTOLF, R.
700 1 $aNEPOMUCENO, A. L.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 31, n. 2, p. 512-521, 2008.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
02/02/2004 |
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Data da última atualização: |
02/02/2004 |
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Autoria: |
PEREIRA, J. A. R.; ALMEIDA, M. M. T. B. |
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Título: |
Educação ambiental e turismo rural na unidade de produção agroecológica: novas oportunidades de mercado. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE AGROECOLOGIA, 1., SEMINÁRIO INTERNACIONAL SOBRE AGROECOLOGIA, 4., SEMINÁRIO ESTADUAL SOBRE AGROECOLOGIA, 5., 2003, Porto Alegre. Resumos... Porto Alegre: PUCRS, 2003. |
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Páginas: |
4 p. |
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Idioma: |
Português |
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Conteúdo: |
A atividade não agrícola é uma estratégia capaz de melhorar o nível de qualidade de vida no campo e retomar a idéia do desenvolvimento rural. Por outro lado, a crescente preocupação mundial com a questão ambiental e o aumento no número de pessoas que desejam realizar atividades junto à natureza favorecem a criação de programas de turisnmo rural e de educação ambiental em unidades de produção agroecológicas, desde que bem construídos. Criando mercados locais e diferenciados, o agro-ecoturismo estimula a consciência ambiental, a inclusão social e o desenvolvimento local, elevando a renda no meio rural e preservando a natureza. |
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Palavras-Chave: |
Rural environment. |
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Thesagro: |
Desenvolvimento Rural; Educação Ambiental. |
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Thesaurus NAL: |
rural development. |
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Categoria do assunto: |
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Marc: |
LEADER 01387naa a2200193 a 4500
001 1623671
005 2004-02-02
008 2003 bl --- 0-- u #d
100 1 $aPEREIRA, J. A. R.
245 $aEducação ambiental e turismo rural na unidade de produção agroecológica$bnovas oportunidades de mercado.
260 $c2003
300 $a4 p.
520 $aA atividade não agrícola é uma estratégia capaz de melhorar o nível de qualidade de vida no campo e retomar a idéia do desenvolvimento rural. Por outro lado, a crescente preocupação mundial com a questão ambiental e o aumento no número de pessoas que desejam realizar atividades junto à natureza favorecem a criação de programas de turisnmo rural e de educação ambiental em unidades de produção agroecológicas, desde que bem construídos. Criando mercados locais e diferenciados, o agro-ecoturismo estimula a consciência ambiental, a inclusão social e o desenvolvimento local, elevando a renda no meio rural e preservando a natureza.
650 $arural development
650 $aDesenvolvimento Rural
650 $aEducação Ambiental
653 $aRural environment
700 1 $aALMEIDA, M. M. T. B.
773 $tIn: CONGRESSO BRASILEIRO DE AGROECOLOGIA, 1., SEMINÁRIO INTERNACIONAL SOBRE AGROECOLOGIA, 4., SEMINÁRIO ESTADUAL SOBRE AGROECOLOGIA, 5., 2003, Porto Alegre. Resumos... Porto Alegre: PUCRS, 2003.
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Embrapa Agrobiologia (CNPAB) |
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