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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
10/10/2011 |
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Data da última atualização: |
30/11/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FREITAS-SILVA, O.; TEIXEIRA, A.; CUNHA, F. Q. da; GODOY, R. L. de O.; VENÂNCIO, A. |
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Afiliação: |
OTNIEL FREITAS SILVA, CTAA; ALESSANDRA TEIXEIRA, UFRRJ; FLAVIO QUITERIO DA CUNHA, CTAA; RONOEL LUIZ DE OLIVEIRA GODOY, CTAA; ARMANDO VENÂNCIO, UNIVERSITY OF MINHO-PT. |
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Título: |
Predominant mycobiota and aflatoxin content in Brazil nuts. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Journal für Verbraucherschutz und Lebensmittelsicherheit, v. 6, n. 4, p. 465-472, 2011. |
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DOI: |
10.1007/s00003-011-0703-6 |
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Idioma: |
Inglês |
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Conteúdo: |
The Brazil nut (Bertholletia excelsa) is an economically important product to the Brazilian Amazon. Currently, its marketing is being affected by the high incidence of aflatoxins (AF) produced by potentially aflatoxigenic fungi associated with its seeds. In this context, the purpose of this study was to determine which part of the nut contributes to contamination by aflatoxins and to identify the mycobiota in Brazil nut samples. Unshelled and shelled nuts were analyzed by measuring the total count of filamentous fungi (Aspergillus sections Flavi, Nigri and Circumdati) in sanitised and non-sanitised treatments. The isolates identified as Aspergillus section Flavi, the major producers of AF, were plated for determination of their aflatoxigenic potential. To perform the AF analysis, samples of Brazil nuts were treated separately. The AF from the shell and kernel were extracted by chloroform and analysed by the HPLC-FD system in isocratic mode. The Aspergillus section Flavi count was 21.67% lower. The production of AF by the isolated fungi was 30% for sanitised and 23.8% for non-sanitised samples. The concentrations obtained of AFB1 and AFG1 were higher than those of AFB2 and AFG2. The AFB1 concentrations of shelled nuts and shell samples were 35.0 and 1.78 ?g/kg, respectively. AFB2 and AFG2 were detected only in shelled nut samples. The HPLC-FD analysis presented limits of detection (LOD) and quantification (LOQ) of 0.2 and 0.4 ?g/kg, respectively. |
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Thesagro: |
Aspergillus flavus; Bertholletia excelsa; Castanha do Pará; Fungo; Micotoxina; Patologia de semente; Segurança alimentar. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02246naa a2200265 a 4500
001 1902807
005 2012-11-30
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1007/s00003-011-0703-6$2DOI
100 1 $aFREITAS-SILVA, O.
245 $aPredominant mycobiota and aflatoxin content in Brazil nuts.
260 $c2011
520 $aThe Brazil nut (Bertholletia excelsa) is an economically important product to the Brazilian Amazon. Currently, its marketing is being affected by the high incidence of aflatoxins (AF) produced by potentially aflatoxigenic fungi associated with its seeds. In this context, the purpose of this study was to determine which part of the nut contributes to contamination by aflatoxins and to identify the mycobiota in Brazil nut samples. Unshelled and shelled nuts were analyzed by measuring the total count of filamentous fungi (Aspergillus sections Flavi, Nigri and Circumdati) in sanitised and non-sanitised treatments. The isolates identified as Aspergillus section Flavi, the major producers of AF, were plated for determination of their aflatoxigenic potential. To perform the AF analysis, samples of Brazil nuts were treated separately. The AF from the shell and kernel were extracted by chloroform and analysed by the HPLC-FD system in isocratic mode. The Aspergillus section Flavi count was 21.67% lower. The production of AF by the isolated fungi was 30% for sanitised and 23.8% for non-sanitised samples. The concentrations obtained of AFB1 and AFG1 were higher than those of AFB2 and AFG2. The AFB1 concentrations of shelled nuts and shell samples were 35.0 and 1.78 ?g/kg, respectively. AFB2 and AFG2 were detected only in shelled nut samples. The HPLC-FD analysis presented limits of detection (LOD) and quantification (LOQ) of 0.2 and 0.4 ?g/kg, respectively.
650 $aAspergillus flavus
650 $aBertholletia excelsa
650 $aCastanha do Pará
650 $aFungo
650 $aMicotoxina
650 $aPatologia de semente
650 $aSegurança alimentar
700 1 $aTEIXEIRA, A.
700 1 $aCUNHA, F. Q. da
700 1 $aGODOY, R. L. de O.
700 1 $aVENÂNCIO, A.
773 $tJournal für Verbraucherschutz und Lebensmittelsicherheit$gv. 6, n. 4, p. 465-472, 2011.
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Embrapa Agroindústria de Alimentos (CTAA) |
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| 1. |  | WARDLE, D. A.; BROWN, V. K.; BEHAN-PELLETIER, V.; ST. JOHN, M.; WOJTOWICZ, T.; BARDGETT, R. D.; BROWN, G. G.; INESON, P.; LAVELLE, P.; PUTTEN, W. H. van der; ANDERSON, J. M.; BRUSSAARD, L.; HUNT, H. W.; PAUL, E. A.; WALL, D. H. Vulnerability to global change of ecosystem goods and services driven by soil biota. In: WALL, D. H. (Ed.). Sustaining biodiversity and ecosystem services in soil and sediments. Washington: Island Press, 2004. p. 101-135. (SCOPE, 64).| Biblioteca(s): Embrapa Soja. |
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| 2. | | PUTTEN, W. H. van der; ANDERSON, J. M.; BARDGETT, R. D.; BEHAN-PELLETIER, V.; BIGNELL, D. E.; BROWN, G. G.; BROWN, V. K.; BRUSSAARD, L.; HUNT, H. W.; INESON, P.; JONES, T. H.; LAVELLE, P.; PAUL, E. A.; ST. JOHN, M.; WARDLE, D. A.; WOJTOWICZ, T.; WALL, D. H. The sustainable delivery of goods and services provided by soil biota. In: WALL, D. H. (Ed.). Sustaining biodiversity and ecosystem services in soil and sediments. Washington: Island Press, 2004. p. 15-43. (SCOPE, 64).| Biblioteca(s): Embrapa Soja. |
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