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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
28/06/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
CAMPOS, L. L.; LANDIM-ALVARENGA, F. C.; IKEDA, T. L.; MONTEIRO, B. A.; MAIA, L.; FREITAS-Dell'AQUA, C. P.; De VITA, B. |
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Afiliação: |
Loreta L. Campos, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Fernanda C. Landim-Alvarenga, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Tatícia L. Ikeda, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Bianca A. Monteiro, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Leandro Maia, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Camila P. Freitas-Dell?Aqua, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Bruna De Vita, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp). |
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Título: |
Isolation, culture, characterization and cryopreservation of stem cells derived from amniotic mesenchymal layer and umbilical cord tissue of bovine fetuses. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 3, p. 278-286, março 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Isolamento, cultura, caracterização e criopreservação de células tronco derivadas de camada amniótica mesenquimal e de tecido do cordão umbilical de fetuses bovinos. |
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Conteúdo: |
Stem cells are undifferentiated cells with a high proliferation potential. These cells canbe characterized by their in vivo ability to self-renew and to differentiate into specialized cell lines. The most used stem cell types, in both human and veterinary fields, are the mesenchymal stem cells (MSC) derived from bone marrow and adipose tissue. Nowadays, there is a great interest in using stem cells derived from fetal tissues, such as amniotic membrane (AM) and umbilical cord tissue (UCT), which can be obtained non-invasively at delivery time. Due to the scarcity of studies in bovine species, the aim of this study was to isolate, characterize, differentiate and cryopreserve MSC derived from the mesenchymal layer of amniotic membrane (AM), for the first time, and umbilical cord tissue (UCT) of dairy cow neonates after assisted delivery (AD) and from fetus at initial third of pregnancy (IT) obtained in slaughterhouse. Cells were isolated by enzymatic digestion of the tissue fragments with 0.1% collagenase solution. Six samples of AM and UCT at delivery time and six samples of AM and UCT at first trimester of pregnancy were subjected to morphology evaluation, imunophenotype characterization, in vitro osteogenic, adipogenic and chondrogenic differentiation and viability analysis after cryopreservation. All samples showed adherence to plastic and fibroblast-like morphology. Immunocytochemistry revealed expression of CD 44, NANOG and OCT-4 and lack of expression of MHC II in MSC from all samples. Flow cytometry demonstrated that cells from all samples expressed CD 44, did not or low expressed CD 34 (AM: IT-0.3%a, AD-3.4%b; UCT: 0.4%, 1.4%) and MHC II (AM: IT-1.05%a, AD-9.7%b; UCT: IT-0.7%a, AD-5.7%b). They were also capable of trilineage mesenchymal differentiation and showed 80% viability after cryopreservation. According to the results, bovine AM and UCT-derived cells, either obtained at delivery time or from slaughterhouse, are a painless and non-invasive source of MSC and can be used for stem cell banking. MenosStem cells are undifferentiated cells with a high proliferation potential. These cells canbe characterized by their in vivo ability to self-renew and to differentiate into specialized cell lines. The most used stem cell types, in both human and veterinary fields, are the mesenchymal stem cells (MSC) derived from bone marrow and adipose tissue. Nowadays, there is a great interest in using stem cells derived from fetal tissues, such as amniotic membrane (AM) and umbilical cord tissue (UCT), which can be obtained non-invasively at delivery time. Due to the scarcity of studies in bovine species, the aim of this study was to isolate, characterize, differentiate and cryopreserve MSC derived from the mesenchymal layer of amniotic membrane (AM), for the first time, and umbilical cord tissue (UCT) of dairy cow neonates after assisted delivery (AD) and from fetus at initial third of pregnancy (IT) obtained in slaughterhouse. Cells were isolated by enzymatic digestion of the tissue fragments with 0.1% collagenase solution. Six samples of AM and UCT at delivery time and six samples of AM and UCT at first trimester of pregnancy were subjected to morphology evaluation, imunophenotype characterization, in vitro osteogenic, adipogenic and chondrogenic differentiation and viability analysis after cryopreservation. All samples showed adherence to plastic and fibroblast-like morphology. Immunocytochemistry revealed expression of CD 44, NANOG and OCT-4 and lack of expression of MHC II in MSC fr... Mostrar Tudo |
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Palavras-Chave: |
Anexo fetal; Cell banking; Célula tronco mesenquimal; Fetal annexes; Holstein cow; Immunophetotype; Imunofenótipo; MHC class II; Vaca holandesa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161149/1/Isolation-culture-characterization.pdf
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Marc: |
LEADER 03217naa a2200313 a 4500
001 2071637
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aCAMPOS, L. L.
245 $aIsolation, culture, characterization and cryopreservation of stem cells derived from amniotic mesenchymal layer and umbilical cord tissue of bovine fetuses.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Isolamento, cultura, caracterização e criopreservação de células tronco derivadas de camada amniótica mesenquimal e de tecido do cordão umbilical de fetuses bovinos.
520 $aStem cells are undifferentiated cells with a high proliferation potential. These cells canbe characterized by their in vivo ability to self-renew and to differentiate into specialized cell lines. The most used stem cell types, in both human and veterinary fields, are the mesenchymal stem cells (MSC) derived from bone marrow and adipose tissue. Nowadays, there is a great interest in using stem cells derived from fetal tissues, such as amniotic membrane (AM) and umbilical cord tissue (UCT), which can be obtained non-invasively at delivery time. Due to the scarcity of studies in bovine species, the aim of this study was to isolate, characterize, differentiate and cryopreserve MSC derived from the mesenchymal layer of amniotic membrane (AM), for the first time, and umbilical cord tissue (UCT) of dairy cow neonates after assisted delivery (AD) and from fetus at initial third of pregnancy (IT) obtained in slaughterhouse. Cells were isolated by enzymatic digestion of the tissue fragments with 0.1% collagenase solution. Six samples of AM and UCT at delivery time and six samples of AM and UCT at first trimester of pregnancy were subjected to morphology evaluation, imunophenotype characterization, in vitro osteogenic, adipogenic and chondrogenic differentiation and viability analysis after cryopreservation. All samples showed adherence to plastic and fibroblast-like morphology. Immunocytochemistry revealed expression of CD 44, NANOG and OCT-4 and lack of expression of MHC II in MSC from all samples. Flow cytometry demonstrated that cells from all samples expressed CD 44, did not or low expressed CD 34 (AM: IT-0.3%a, AD-3.4%b; UCT: 0.4%, 1.4%) and MHC II (AM: IT-1.05%a, AD-9.7%b; UCT: IT-0.7%a, AD-5.7%b). They were also capable of trilineage mesenchymal differentiation and showed 80% viability after cryopreservation. According to the results, bovine AM and UCT-derived cells, either obtained at delivery time or from slaughterhouse, are a painless and non-invasive source of MSC and can be used for stem cell banking.
653 $aAnexo fetal
653 $aCell banking
653 $aCélula tronco mesenquimal
653 $aFetal annexes
653 $aHolstein cow
653 $aImmunophetotype
653 $aImunofenótipo
653 $aMHC class II
653 $aVaca holandesa
700 1 $aLANDIM-ALVARENGA, F. C.
700 1 $aIKEDA, T. L.
700 1 $aMONTEIRO, B. A.
700 1 $aMAIA, L.
700 1 $aFREITAS-Dell'AQUA, C. P.
700 1 $aDe VITA, B.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 3, p. 278-286, março 2017.
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Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agropecuária Oeste. Para informações adicionais entre em contato com cpao.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
16/01/2024 |
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Data da última atualização: |
16/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
AVILA, C. J.; GARCIA, R. A.; FRAGOSO, L. H. M.; DAPONT, J. V. K. |
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Afiliação: |
CREBIO JOSE AVILA, CPAO; RODRIGO ARROYO GARCIA, CPAO; LUIS HENRIQUE MAIDANA FRAGOSO, CENTRO UNIVERSITÁRIO DA GRANDE DOURADOS, DOURADOS; JOÃO VITOR KLEIN DAPONT, CENTRO UNIVERSITÁRIO DA GRANDE DOURADOS, DOURADOS. |
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Título: |
Soja: refúgio necessário. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Cultivar Grandes Culturas, ano 25, n. 292, p. 8-11, 2023. |
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Idioma: |
Português |
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Palavras-Chave: |
Lagarta desfolhadora; Soja bt. |
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Thesagro: |
Manejo; Variedade. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 00520naa a2200193 a 4500
001 2160887
005 2024-01-16
008 2023 bl uuuu u00u1 u #d
100 1 $aAVILA, C. J.
245 $aSoja$brefúgio necessário.$h[electronic resource]
260 $c2023
650 $aManejo
650 $aVariedade
653 $aLagarta desfolhadora
653 $aSoja bt
700 1 $aGARCIA, R. A.
700 1 $aFRAGOSO, L. H. M.
700 1 $aDAPONT, J. V. K.
773 $tCultivar Grandes Culturas, ano 25$gn. 292, p. 8-11, 2023.
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Embrapa Agropecuária Oeste (CPAO) |
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