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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
24/12/2008 |
Data da última atualização: |
15/01/2009 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA-PINHATI, A. C.; FREITAS-ÁSTUA, J.; AMARAL, A. M. do; MACHADO, M. A. |
Afiliação: |
A. C. Silva-Pinhati, CCSM/IAC; Juliana Freitas-Ástua, CNPMF/CCSM/IAC; A. M. do Amaral, CCSM/IAC; M. A. Machado, CCSM/IAC. |
Título: |
Expression of genes associate to biosynthesis of flavonoids of Citrus sinensis. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan, China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 161. P 132. |
Idioma: |
Inglês |
Conteúdo: |
Flavonoids constitute a major group of secondary metabolites and they are directly associated with citrus fruit quality. In plants, these compounds are involved in the protection against the biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. Flavones represent one of the most abundant and important classes of flavonoids, occurring in almost all plant tissues. The formation of flavones is catalyzed by flavone synthase I or flavone synthase II (FNSII). One of the main enzymes in the flavonol biosynthesis pathway is the flavanone-3-hydroxylase (F3H). In a previous study, we identified putative novel genes involved in the synthesis of flavones and flavonols in Citrus sinensis through expressed sequence tags (ESTs) of the CitEST database. Even though flavones have been found in several species and varieties within the citrus genus, this is the first study to report the presence of two copies of the fnsII gene in sweet orange. Moreover, in addition to the available Citf3h gene sequence, we identified another f3h transcript in C. sinensis. Because of the importance of having been found putative novel copies of genes involved in flavonoids biosynthesis, here we report the characterization of such gene expression patterns in different tissues and citrus fruit developmental stages, studied by real time quantitative PCR for the validation of results observed in silico analysis. Some of these genes or copies of genes should be used for genetic transformation of citrus to improve the fruit quality. MenosFlavonoids constitute a major group of secondary metabolites and they are directly associated with citrus fruit quality. In plants, these compounds are involved in the protection against the biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. Flavones represent one of the most abundant and important classes of flavonoids, occurring in almost all plant tissues. The formation of flavones is catalyzed by flavone synthase I or flavone synthase II (FNSII). One of the main enzymes in the flavonol biosynthesis pathway is the flavanone-3-hydroxylase (F3H). In a previous study, we identified putative novel genes involved in the synthesis of flavones and flavonols in Citrus sinensis through expressed sequence tags (ESTs) of the CitEST database. Even though flavones have been found in several species and varieties within the citrus genus, this is the first study to report the presence of two copies of the fnsII gene in sweet orange. Moreover, in addition to the available Citf3h gene sequence, we identified another f3h transcript in C. sinensis. Because of the importance of having been found putative novel copies of genes involved in flavonoids biosynthesis, here we report the characterization of such gene expression patterns in different tissues and citrus fruit developmental stages, studied by real time quantitative PCR for the validation of results observed in silico analysis. Some of these genes or copies of genes should be used... Mostrar Tudo |
Palavras-Chave: |
Citros; Melhoramento genetico. |
Thesagro: |
Genética Vegetal. |
Categoria do assunto: |
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Marc: |
LEADER 02225naa a2200193 a 4500 001 1655358 005 2009-01-15 008 2008 bl uuuu u00u1 u #d 100 1 $aSILVA-PINHATI, A. C. 245 $aExpression of genes associate to biosynthesis of flavonoids of Citrus sinensis. 260 $c2008 520 $aFlavonoids constitute a major group of secondary metabolites and they are directly associated with citrus fruit quality. In plants, these compounds are involved in the protection against the biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. Flavones represent one of the most abundant and important classes of flavonoids, occurring in almost all plant tissues. The formation of flavones is catalyzed by flavone synthase I or flavone synthase II (FNSII). One of the main enzymes in the flavonol biosynthesis pathway is the flavanone-3-hydroxylase (F3H). In a previous study, we identified putative novel genes involved in the synthesis of flavones and flavonols in Citrus sinensis through expressed sequence tags (ESTs) of the CitEST database. Even though flavones have been found in several species and varieties within the citrus genus, this is the first study to report the presence of two copies of the fnsII gene in sweet orange. Moreover, in addition to the available Citf3h gene sequence, we identified another f3h transcript in C. sinensis. Because of the importance of having been found putative novel copies of genes involved in flavonoids biosynthesis, here we report the characterization of such gene expression patterns in different tissues and citrus fruit developmental stages, studied by real time quantitative PCR for the validation of results observed in silico analysis. Some of these genes or copies of genes should be used for genetic transformation of citrus to improve the fruit quality. 650 $aGenética Vegetal 653 $aCitros 653 $aMelhoramento genetico 700 1 $aFREITAS-ÁSTUA, J. 700 1 $aAMARAL, A. M. do 700 1 $aMACHADO, M. A. 773 $tIn: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan, China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 161. P 132.
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Registros recuperados : 111 | |
3. | | BASTIANEL, M.; NOVELLI, V. M.; FREITAS-ASTUA, J. de. Field assessments reveal different levels of susceptibility among mandarins and hybrids to Citrus Leprosis Virus c. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 129.PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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5. | | FREITAS-ÁSTUA, J.; NICOLINI, F.; BASTIANEL, M.; KITAJIMA, E. W. Interações entre o vírus da leprose dos citros e seu vetor Brevipalpus phoenicis. Summa Phytopathologica, Botucatu, v. 34, , p. 128, fev. 2008. Suplemento. Edição dos Resumos do XXXI Congresso Paulista de Fitopatologia, Campinas, fev. 2008.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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7. | | KUBO, K. S.; FREITAS-ÁSTUA, J.; KITAGIMA, E. W. Detecção do Clerodendron chlorotic sport virus in planta e no ácaro vetor. Summa Phytopathologica, Botucatu, v. 34, , p. 97, fev. 2008. Suplemento. Edição dos Resumos do XXXI Congresso Paulista de Fitopatologia, Campinas, fev. 2008.
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Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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8. | | MASCARENHAS, G.; BARBOSA, C. de J.; KITAJIMA, E. W.; FREITAS-ÁSTUA, J. Alta eficiência de transmissão mecânica e por vetor do vírus da pinta verde (PFGSV) para maracujazeiro. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 911.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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11. | | RODRIGUES, A. S.; BARBOSA, C. de J.; SOARES FILHO, W. dos S.; FREITAS-ASTUA, J. de. Citrus rootstock hybrids tolerants to citrus tristeza virus and Bahia bark scaling disease. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 106_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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12. | | KITAJIMA, E. W.; CHAGAS, C. M.; HARAKAWA, R.; FREITAS-ASTÚA, J.; RODRIGUES, J. C. V. Citrus leprosis in Florida may have been of the nuclear type. Virus Reviews & Research, Rio de Janeiro, v. 14, p. 90, 2009. Suplemento 1. Trabalho apresentado no XX Encontro Nacional de Virologia, Brasília, DF. Resumo 098.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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13. | | TASSI, A. D.; FREITAS-ASTÚA, J.; JADÃO, A.; KITAJIMA, E. W. Citrus leprosis virus (CILV-C) vector relationship assessed by bean as test plant. Virus Reviews & Research, Rio de Janeiro, v. 14, p. 90, 2009. Suplplement 1. Trabalho apresentado no XX Encontro Nacional de Virologia, Brasília, DF. Resumo 097Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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14. | | FARIA, A. S.; BASTIANEL, M.; MIRANDA, M.; NOVELLI, V. M.; FREITAS-ÁSTUA, J. Eficiência de transmissão do vírus da leprose dos citros nas diferentes fases ativas do ácaro vetor. Summa Phytopathologica, Botucatu, v. 34, , p. 58, fev. 2008. Suplemento. Edição dos Resumos do XXXI Congresso Paulista de Fitopatologia, Campinas, fev. 2008.
R 171Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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16. | | FREITAS-ÁSTUA, J.; BASTIANEL, M.; NICOLINI, F.; SCHONS, J.; KITAJIMA, E. W.; MACHADO, M. A. Evidences to support that Citrus leprosis virus and its mite vector interact in a circulative - but not propagative - manner. In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan, China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 237-238. P349Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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18. | | NICOLINI, F.; BASTIANEL, M.; FREITAS-ÁSTUA, J.; ANTONIOLI-LUIZON, R.; SCHONS, J.; MACHADO, M. A. Quantificação do vírus da leprose dos citros no hospedeiro vegetal. Summa Phytopathologica, Botucatu, v. 34, , p. 66, fev. 2008. Suplemento. Edição dos Resumos do XXXI Congresso Paulista de Fitopatologia, Campinas, fev. 2008.
R 199Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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20. | | ANTONIOLI-LUIZON, R.; FREITAS-ÁSTUA, J.; REZENDE, J. A. M.; MACHADO, M. A.; KITAJIMA, E. W. Detecção específica do vírus da pinta verde do maracujazeiro por RT-PCR. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 907.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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