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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/01/2011 |
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Data da última atualização: |
19/01/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, M. F. e; MIROLI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; FREITAS ASTUA, J. de; MACHADO, M. A. |
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Afiliação: |
Marina França e Silva, CNPDIA; Débora Marcondes Bastos Pereira Miroli, CNPDIA; Marcelo C. B. Cardinali, CNPDIA; Yvonne P. Mascarenhas, USP; Polyanna kelly Martins, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Marcos Antonio Machado, APTA. |
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Título: |
Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
120_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
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Conteúdo: |
Several diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early citrus greening detection. MenosSeveral diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early ci... Mostrar Tudo |
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Palavras-Chave: |
Candidatus Liberibater asiaticus; Plant disease. |
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Thesagro: |
Bactéria; Doença de Planta; Fruta Cítrica; Greening. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02492nam a2200265 a 4500
001 1872725
005 2011-01-19
008 2010 bl uuuu u00u1 u #d
100 1 $aSILVA, M. F. e
245 $aApplication of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease.
260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010
500 $a120_PS2 Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010
520 $aSeveral diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early citrus greening detection.
650 $aBactéria
650 $aDoença de Planta
650 $aFruta Cítrica
650 $aGreening
653 $aCandidatus Liberibater asiaticus
653 $aPlant disease
700 1 $aMIROLI, D. M. B. P.
700 1 $aCARDINALI, M. C. B.
700 1 $aMASCARENHAS, Y. P.
700 1 $aMARTINS, P. K.
700 1 $aFREITAS ASTUA, J. de
700 1 $aMACHADO, M. A.
Download
Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 111 | |
| 61. |  | VENANCIO, A. L.; CARDINALLI, M. C. B.; SILVA, M. F.; MILORI, D. M. B. P.; FERREIRA, E. J.; MARTINS, P. K.; FREITAS-ASTÚA, J. Avaliação da precocidade do diagnóstico do Greening utilizando espectroscopia de fluorescência induzida por laser (LIFS). In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 36. (Embrapa Instrumentação Agropecuária. Documentos, 50).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 62. | | NICOLINI, F.; BASTIANEL, M.; SCHONS, J.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; PEREIRA, J. A.; KUBO, K. S.; NOVELLI, V. M.; MACHADO, M. A.; FREITAS-ASTÚA, J. Comparação entre os títulos de vírus transmitidos por ácaros Brevipalpus por RT-qPCR. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| 63. | | BERGER, I. J.; FREITAS-ÁSTUA, J.; SILVA-PINHATI, A. C. O.; BASILIO-PALMIERI, A. C.; LUCHETA, A. R.; NOVELLI, V. M.; MACHADO, M. A. Chalcone isomerase is likely encoded by a multigene family in citrus sinensis. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego: Illumina, 2008. P21| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 64. | | FREITAS-ÁSTUA, J.; BASTIANEL, M.; LOCALI-FABRIS, E. C.; NOVELLI, V. M.; SILVA-PINHATI, A. C.; BASÍLIO-PALMIERI, A. C.; TARGON, M. L. P. N.; MACHADO, M. A. Differentially expressed stress-related genes in the compatible citrus-Citrus leprosis virus interaction. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 980-990, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 65. | | CORREA, P. H.; MARTINS, P. K.; ARENA, G. D.; MIGUEL, M. C.; MUTTI, H. S.; MACHADO, M. A.; FREITAS-ASTUA, J. Diagnóstico molecular de huanglongbing dos citros, via qPCR, em mudas de valência sobre Swingle. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1867| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 66. | | MILORI, D. M. B. P.; BOAS, P. R. V.; VEMÂNCIO, A. L.; FERREIRA, E. J.; MARTINS, P. K.; FREITAS-ASTUA, J.; BRESOLIN, J.; CARDINALI, M. C. B. Diagnóstico de Citrus Greening (HLB) utilizando espectroscopia de fluorescência induzida por laser (LIFS). In: INAMASU, R. Y.; NAIME, J. de M.; RESENDE, A. V. de; BASSOI, L. H.; BERNARDI, A. de C. (Ed.). Agricultura de precisão: um novo olhar. São Carlos, SP: Embrapa Instrumentação, 2011. p. 78-81.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Instrumentação. |
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| 68. | | COSTA, F. M.; FREITAS-ASTUA, J.; BASTIANEL, M.; LOCALI-FABRIS, E.; MACHADO, M. A.; KUBO, K. S.; GONDIM JÚNIOR, M. G. C.; NOVELLI, V. M.; PEREIRA, J. A. Detecção do vírus da mancha anelar do cafeeiro (CoRSV) nas diferentes fases do ácaro vetor Brevipalpus phoenicis. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 919.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 69. | | KUBO, K. S.; NOVELLI, V. M.; BASTIANEL, M.; LOCALI-FABRIS, E. C.; ANTONIOLI-LUIZON, R.; MACHADO, M. A.; FREITAS-ASTUA, J. Detection of Brevipalpus-transmitted viruses in their mite vectors by RT-PCR. Experimental Applied Acarology, Dordrechet, v. 54, n. 1, jan. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 70. | | BASTIANEL, M.; FREITAS- ASTÚA, J.; NICOLINI, F.; GUIDOTTI, A. R.; FARIAS, A. S.; NOVELLI, V. M.; KITAJIMA, E. W.; MACHADO, M. A. Interactions between Citrus leprosis virus and its vector Brevipalpus phoenicis (Acari: tenuipalpidae). In: EUROPEAN CONGRESS OF ACAROLOGY INTEGRATIVE ACAROLOGY, 6., Abstracts... Montpellier: EURAAC, 2008. p. 19| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 71. | | CARDINALI, M. C. B.; VENÂNCIO, A. L.; KUBOTA, T. M. K.; MILORI, D. M. B. P.; FERREIRA, E. J.; MARTINS, P. K.; FREITAS-ASTÚA, J. Evaluation of early diagnosis of citrus greening using laser-induced fluorescence spectroscopy. Citrus research & technology, Cordeirópolis, v. 31, suplemento, p. 108, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas. 1CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação; Embrapa Mandioca e Fruticultura. |
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| 72. | | PEREIRA, F. M. V.; MILORI, D. M. B. P.; VENÂNCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; FREITAS-ASTÚA, J. Evaluation of the effects of candidatus liberibacter asiaticus on inoculated citrus plants using laser-induced breakdown spectroscopy (LIBS) and chemometrics tools. Talanta, Oxford, v. 83, p. 351-356, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 73. | | KUBO, K. S.; STUART, R. M.; FREITAS-ASTÚA, J.; ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; COLETTA-FILHO, H. D.; MACHADO, M. A.; KITAJIMA, E. W. Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid. Archives of Virology, v. 154, n. 6, p. 1009-1014, mai. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 74. | | PEREIRA, F. M. V.; MILORI, D. M. B. P.; VENANCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; FREITAS-ASTÚA, J. Evaluations of the effects of candidatus liberibacter asiaticus on inoculated citrus plants using laser-induced breakdown spectroscopy and chemometric tools. Citrus research & technology, Cordeirópolis, v. 31, suplemento, p. 95, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas. 1CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação; Embrapa Mandioca e Fruticultura. |
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| 75. | | FREITAS ASTUA, J. de; KUBO, K. S.; KITAJIMA, E. W.; BASTIANEL, M.; COSTA, F. M.; NOVELLI, V. M.; MACHADO, M. A. The leprosis pathosystem: interactions between host, virus and vector. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS,18., CAMPINAS, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 009 PSO
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 76. | | NOVELLI, V. M.; NUNES, M. A.; MENDONÇA, R. S. de; FREITAS ASTUA, J.; SINICO, T. E.; LIN, Y. C.; LE, P.; VAN DE PEER, Y.; NAVIA, D. Genome annotation of the flat mite Brevipalpus yothersi Baker (Tenuipalpidae). In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 8., 2016, València. [Abstracts...]. Valencia: Universitat Politècnica de Valencia, 2016. p. 51.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 77. | | NOVELLI, V. M.; NUNES, M. A.; MENDONÇA, R. S. de; FREITAS ASTUA, J.; SINICO, T. E.; LIN, Y. C.; LE, P.; VAN DE PEER, Y.; NAVIA, D. Genome annotation of the flat mite Brevipalpus yothersi Baker (Tenuipalpidae). In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 8., 2016, València. [Abstracts...]. Valencia: Universitat Politècnica de Valencia, 2016. p. 51.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 78. | | PEREIRA, F. M. V.; MILORI, D. M. B. P.; PEREIRA-FILHO, E. R.; VENÂNCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; FREITAS-ASTÚA, J. Fluorescence images combined to chemometrics for the investigation of plants inoculated with candidatus liberibacter asiaticus In: CHEMOMETRICS IN ANALYTICAL CHEMISTRY - CAC, 12., 2010, Antwerp, Belgium. Book of abstracts... Antwerp: Universiteit Antwerpen, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 79. | | PEREIRA, F. M. V.; MILORI, D. M. B. P.; PEREIRA-FILHO, E. R.; VENANCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; FREITAS-ASTUA, J. Fluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection. Analytical methods, [S. l.], v. 3, p. 552-556, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Instrumentação; Embrapa Mandioca e Fruticultura. |
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| 80. | | GUIDETTI-GONZALEZ, S.; FREITAS-ÁSTUA, J.; AMARAL, A. M. do; MARTINS, N. F.; MEHTA, A.; SILVA, M. S. S.; CARRER, H. Genes associated with hypersensitive response (HR) in the citrus EST database (CitEST). Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 943-967, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 111 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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