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Registro Completo |
Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Alimentos e Territórios. |
Data corrente: |
17/11/2023 |
Data da última atualização: |
23/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, A. T. L. dos; CARNEIRO, J. N. P.; CRUZ, R. P. da; SALES, D. L.; PINHEIRO, J. C. A.; FREITAS, M. A. de; KERNTOPF, M. R.; DELMONDES, G. de A.; RIBEIRO, P. R. V.; BRITO, E. S. de; BATISTA, F. L. A.; MAGALHÃES, F. E. A.; PITA NETO, I. C.; BRAGA, M. F. B. M.; KOWALSKI, R.; KOWALSKA, G.; SZOPA, A.; BAJ, T.; COUTINHO, H. D. M. |
Afiliação: |
ANTONIA THASSYA LUCAS DOS SANTOS, UNIVERSIDADE REGIONAL DO CARIRI; JOARA NÁLYDA PEREIRA CARNEIRO, UNIVERSIDADE REGIONAL DO CARIRI; RAFAEL PEREIRA DA CRUZ, UNIVERSIDADE REGIONAL DO CARIRI; DÉBORA LIMA SALES, UNIVERSIDADE REGIONAL DO CARIRI; JACQUELINE COSMO ANDRADE-PINHEIRO, UNIVERSIDADE REGIONAL DO CARIRI; MARIA AUDILENE DE FREITAS, UNIVERSIDADE FEDERAL DE PERNAMBUCO; MARTA REGINA KERNTOPF, UNIVERSIDADE REGIONAL DO CARIRI; GYLLYANDESON DE ARAÚJO DELMONDES, UNIVERSIDADE REGIONAL DO CARIRI; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; EDY SOUSA DE BRITO, CNAT; FRANCISCO LUCAS ALVES BATISTA, UNIVERSIDADE REGIONAL DO CARIRI; FRANCISCO ERNANI ALVES MAGALHÃES, UNIVERSIDADE ESTADUAL DO CEARÁ; IVO CAVALCANTE PITA NETO, FACULDADE CECAPE; MARIA FLAVIANA BEZERRA MORAIS-BRAGA, UNIVERSIDADE REGIONAL DO CARIRI; RADOSŁAW KOWALSKI, UNIWERSYTET PRZYRODNICZNY W LUBLINIE; GRAZYNA KOWALSKA, UNIWERSYTET PRZYRODNICZNY W LUBLINIE; ALEKSANDRA SZOPA, UNIWERSYTET MEDYCZNY W LUBLINIE; TOMASZ BAJ, UNIWERSYTET MEDYCZNY W LUBLINIE; HENRIQUE DOUGLAS MELO COUTINHO, UNIVERSIDADE REGIONAL DO CARIRI. |
Título: |
UPLC-MS-ESI-QTOF analysis and antifungal activity of aqueous extracts of Spondias tuberosa. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Molecules, Basel, v. 28, n. 1, art. 305, Jan. 2023. |
Páginas: |
14 p. |
DOI: |
https://doi.org/10.3390/molecules28010305 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to identify the chemical composition of the Spondias tuberosa aqueous leaf and root extracts (EALST and EARST) and to evaluate their effect, comparatively, against opportunistic pathogenic fungi. Ultra-Performance Liquid Chromatography Coupled to a Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF) was employed for chemical analysis. Candida albicans and C. tropicalis standard strains and clinical isolates were used (CA INCQS 40006, CT INCQS 40042, CA URM 5974, and CT URM 4262). The 50% Inhibitory Concentration for the fungal population (IC50) was determined for both the intrinsic action of the extracts and the extract/fluconazole (FCZ) associations. The determination of the Minimum Fungicidal Concentration (MFC) and the verification of effects over fungal morphological transitions were performed by subculture in Petri dishes and humid chambers, respectively, both based on micro-dilution. UPLC-MS-ESI-QTOF analysis revealed the presence of phenolic and flavonoid compounds. The association of the extracts with fluconazole, resulted in IC50 values from 2.62 µg/mL to 308.96 µg/mL. The MFC of the extracts was ?16,384 µg/mL for all tested strains, while fluconazole obtained an MFC of 8192 µg/mL against C. albicans strains. A reduction in MFC against CA URM 5974 (EALST: 2048 µg/mL and EARST: 1024 µg/mL) occurred in the extract/fluconazole association. |
Palavras-Chave: |
Popular medicine. |
Thesagro: |
Cromatografia; Spondias Tuberosa; Umbu. |
Thesaurus Nal: |
Electrospray ionization mass spectrometry; Flavonoids; Fluconazole; Mass spectrometry; Ultra-performance liquid chromatography. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159154/1/uplc-ms-esi-qtof-analysis-and-antifungal-activity-of-aqueous-extracts-of-spondias-tuberosa-2023.pdf
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Marc: |
LEADER 02772naa a2200469 a 4500 001 2159154 005 2024-01-23 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/molecules28010305$2DOI 100 1 $aSANTOS, A. T. L. dos 245 $aUPLC-MS-ESI-QTOF analysis and antifungal activity of aqueous extracts of Spondias tuberosa.$h[electronic resource] 260 $c2023 300 $a14 p. 520 $aThis study aimed to identify the chemical composition of the Spondias tuberosa aqueous leaf and root extracts (EALST and EARST) and to evaluate their effect, comparatively, against opportunistic pathogenic fungi. Ultra-Performance Liquid Chromatography Coupled to a Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF) was employed for chemical analysis. Candida albicans and C. tropicalis standard strains and clinical isolates were used (CA INCQS 40006, CT INCQS 40042, CA URM 5974, and CT URM 4262). The 50% Inhibitory Concentration for the fungal population (IC50) was determined for both the intrinsic action of the extracts and the extract/fluconazole (FCZ) associations. The determination of the Minimum Fungicidal Concentration (MFC) and the verification of effects over fungal morphological transitions were performed by subculture in Petri dishes and humid chambers, respectively, both based on micro-dilution. UPLC-MS-ESI-QTOF analysis revealed the presence of phenolic and flavonoid compounds. The association of the extracts with fluconazole, resulted in IC50 values from 2.62 µg/mL to 308.96 µg/mL. The MFC of the extracts was ?16,384 µg/mL for all tested strains, while fluconazole obtained an MFC of 8192 µg/mL against C. albicans strains. A reduction in MFC against CA URM 5974 (EALST: 2048 µg/mL and EARST: 1024 µg/mL) occurred in the extract/fluconazole association. 650 $aElectrospray ionization mass spectrometry 650 $aFlavonoids 650 $aFluconazole 650 $aMass spectrometry 650 $aUltra-performance liquid chromatography 650 $aCromatografia 650 $aSpondias Tuberosa 650 $aUmbu 653 $aPopular medicine 700 1 $aCARNEIRO, J. N. P. 700 1 $aCRUZ, R. P. da 700 1 $aSALES, D. L. 700 1 $aPINHEIRO, J. C. A. 700 1 $aFREITAS, M. A. de 700 1 $aKERNTOPF, M. R. 700 1 $aDELMONDES, G. de A. 700 1 $aRIBEIRO, P. R. V. 700 1 $aBRITO, E. S. de 700 1 $aBATISTA, F. L. A. 700 1 $aMAGALHÃES, F. E. A. 700 1 $aPITA NETO, I. C. 700 1 $aBRAGA, M. F. B. M. 700 1 $aKOWALSKI, R. 700 1 $aKOWALSKA, G. 700 1 $aSZOPA, A. 700 1 $aBAJ, T. 700 1 $aCOUTINHO, H. D. M. 773 $tMolecules, Basel$gv. 28, n. 1, art. 305, Jan. 2023.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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12. | | MIRANDA, R. A. de; GARCIA, J. C.; FREITAS, M. S. Diversificação e evolução das exportações brasileiras de milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 30.; SIMPÓSIO SOBRE LEPDÓPTEROS COMUNS A MILHO, SOJA E ALGODÃO, 1., 2014, Salvador. Eficiência nas cadeias produtivas e o abastecimento global: resumos expandidos. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2014. 1 CD-ROM. Trabalho publicado também no 7º Seminário de Iniciação Científica PIBIC/BIC Júnior, 2014, Sete Lagoas.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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17. | | FREITAS, M. A.; MACHADO, J. C.; ANDRADE, A. C. Molecular characterization of Stenocarpella macrospora (Earle) Sutton and Stenocarpella maydis (Berk Sutton) isolates. Fitopatologia Brasileira, Brasília, DF, v. 32, supl., p. S307, ago. 2007. Resumo 0995. Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, PR, ago. 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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