| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
13/04/2011 |
|
Data da última atualização: |
13/04/2011 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
FRANCO, O. L.; ANDRADE, A. E.; BARROS, E. V. S. A.; SA, M. F. G. de; CARNEIRO, R. M. D. G.; CARNEIRO, R.; EIRA, M. T. S. da; ROCHA, T. L.; REIS, A. M. dos. |
|
Afiliação: |
UNIVERSIDADE CATÓLICA DE BRASÍLIA; MARIA FATIMA GROSSI DE SA, CENARGEN; REGINA MARIA DECHECHI G CARNEIRO, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; THALES LIMA ROCHA, CENARGEN; ANGELA MEHTA DOS REIS, CENARGEN. |
|
Título: |
Comparison of different staining methods for coffee proteomic analysis. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Proteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analysis revealed an enhanced number of protein spots, as well as differentially expressed proteins, when CBB was used. A total of approximately 70 and 100 spots were observed in silver and CBB stained gels, respectively. Moreover, 18 differentially expressed proteins were observed in CBB gels, and only 8 in the silver stained gels. This report showed that the staining method was crucial for an optimized protein analysis of coffee. Similar results were obtained for cotton roots and therefore these results may be extended to other plant species in order to better understand the host-pathogen interaction. MenosProteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analys... Mostrar Tudo |
|
Palavras-Chave: |
Bidimensional electrophoresis; Proteomic method. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32592/1/Comparison-of-Different.pdf
|
|
Marc: |
LEADER 02933nam a2200229 a 4500
001 1885752
005 2011-04-13
008 2007 bl uuuu u00u1 u #d
100 1 $aFRANCO, O. L.
245 $aComparison of different staining methods for coffee proteomic analysis.$h[electronic resource]
260 $aIn:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.$c2007
520 $aProteomic methods, such as bidimensional electrophoresis (2-DE) and mass spectrometry, have been extensively used for the study of protein differential expression in several plants including Arabidopsis thaliana, rice and wheat. Specifically in the 2-DE method, deep attention must be given to the protein staining technique, which often involves silver nitrate or Coomassie Brilliant Blue (CBB). Silver staining is usually preferred over CBB due to the higher sensitivity obtained. Nevertheless, silver-staining resolution could significantly vary according to the studied organism and more specifically to the researched tissue. In Coffea spp., 2-DE analysis has been rarely employed. Some studies of protein expression have been reported in this culture mainly involving the biosynthesis of caffeine and metabolism during seed germination. The study of the global protein expression in coffee plants in response to biotic stress conditions had not been reported until now. Phytonematode infection can be considered one of the most important biotic stresses that affect coffee production and Meloidogyne paranaensis is one of the major nematode species that infects coffee plants. In this report, the protein expression of infected- and non-infected roots of coffee (Coffea canephora) were analyzed and the protein pattern determined by 2-DE. Gels were stained with silver nitrate or CBB, in order to obtain an optimized method for proteomic analysis of plant-nematode interaction. The 2-DE analysis revealed an enhanced number of protein spots, as well as differentially expressed proteins, when CBB was used. A total of approximately 70 and 100 spots were observed in silver and CBB stained gels, respectively. Moreover, 18 differentially expressed proteins were observed in CBB gels, and only 8 in the silver stained gels. This report showed that the staining method was crucial for an optimized protein analysis of coffee. Similar results were obtained for cotton roots and therefore these results may be extended to other plant species in order to better understand the host-pathogen interaction.
653 $aBidimensional electrophoresis
653 $aProteomic method
700 1 $aANDRADE, A. E.
700 1 $aBARROS, E. V. S. A.
700 1 $aSA, M. F. G. de
700 1 $aCARNEIRO, R. M. D. G.
700 1 $aCARNEIRO, R.
700 1 $aEIRA, M. T. S. da
700 1 $aROCHA, T. L.
700 1 $aREIS, A. M. dos
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 157 | |
| 21. |  | PEREIRA, J. L.; FRANCO, O. L.; PAULINO, L.; PAPPAS, G. J.; BLOCH JUNIOR, C.; GRATTAPAGLIA, D.; NORONHA, E. F. Comparative xylem proteome of Eucalyptus grandis and Eucalyptus globulus. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|   |
| 25. | | OLIVEIRA NETO, O. B.; CORDEIRO, M. C. R.; MELO, F. R.; FRANCO, O. L.; SA, M. F. G. de. Characterization of midgut proteinase activity in Boll weevil (Anthonomus grandis, Bohema, 1943). In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 28., 1999, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1999. p.36 E-72. Producao cientifica da Embrapa Recursos Geneticos e Biotecnologia.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 26. | | OLIVEIRA NETO, O. B.; CORDEIRO, M. C. R.; MELO, F. R.; FRANCO, O. L.; SA, M. F. G. de. Characterization of midgut proteinase activity in boll weevil (anthonomus grandis, Bohema, 1943). In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 28., 1999, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1999. p.36E-72| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 27. | | PELEGRINI, P. B.; MOREIRA, I. C. A.; MUNIZ, M. A. R.; BLOCH JUNIOR, C.; NORONHA, E. F.; FRANCO, O. L. Biochemical characterization of novel peptides from passion fruit seeds (Passiflora edulis) active against filamentous fungi. In: REUNIÃO ANUAL: SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 34., 2005, Águas de Lindóia. Programas e resumos... São Paulo: Sociedade Brasileira de Bioquímica e Biologia Molecular, 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 30. | | SANTOS, C.; MAXIMIANO, M. R.; RIBEIRO, D. G.; OLIVEIRA-NETO, O. B.; MURAD, A. M.; FRANCO, O. L.; MEHTA, A. Differential accumulation of Xanthomonas campestris pv. campestris proteins during the interaction with the host plant: contributions of an in vivo system. Proteomics, v. 17, n, 12, 2017. 1700086.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 31. | | CORRÊA, A. S. R.; MEHTA, A.; PELEGRINI, P. B.; SANTOS, M. F.; DOMONT, G.; FRANCO, O. L. Differential expression identification of Coffea arabica proteins through the somatic embryogenesis by proteomic analysis. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 32. | | FRANCO, O. L.; RIDGEN, D. J.; MELO, F. R.; BLOCH JUNIOR, C.; GROSSI-de- SÁ, M. F. Inibidores de alpha-amilase do trigo ativos contra alpha-amilases de bruquídeos e explicação estrutural das especificidades observadas. In: WORKSHOP DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 4., 1999, Brasília. Anais: resumos dos trabalhos. Brasília, DF: EMBRAPA-CENARGEN, 1999. p. 54.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 33. | | SILVA, D. P.; CASADO FILHO, E. L.; FARIAS, L. R.; BLOCH JÚNIOR, C.; NORONHA, E. F.; FRANCO, O. L. Inibidores ativos contra ?-amilases de Callosobruchus maculatus provenientes de sementes de sucupira branca Pterodon pubescens. In: ENCONTRO DA SOCIEDADE BRASILEIRA DE MELHORAMENTO DE PLANTAS REGIONAL DF, 1., 2005, Brasília, DF. [Anais...]. Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 110. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 144).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 34. | | MELO, F. R.; FRANCO, O. L.; BLOCH JUNIOR, C.; SILVA, M. C. M.; SA, M. F. G. de. Isolation of cysteine proteinases from acanthoscelides obtectus say (coleoptera, bruchidae). In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 28., 1999, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1999. p.34E-60.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 35. | | MALDANER, F. R.; VASQUES, R.; FRANCO, O. L.; ARAGAO, F. J. L.; RESENDE, R. O.; NAGATA, T. Expression of dengue virus multiepitope antigen in plant using the PVX vector. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 70.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 37. | | FRANCO, O. L.; SA, M. F. G. de; SALES, M. P.; MELLO, L. V.; OLIVEIRA, A. S.; RIGDEN, D. J. Overlapping binding sites for trypsin and papain on a kunitz-type proteinase inhibitor from Prosopis juliflora. Protein: Structure, Function, and Genetics, v. 49, p. 335-341, 2002.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 38. | | PEREIRA, C. D.; MURAD, A. M.; COSTA, R. T.; BLOCH JUNIOR, C.; SILVA, L. P. da; FRANCO, O. L. Proteomical analysis of Beauveria bassiana secretion in the presence of bruchid Callosobruchus maculatus. In: ANNUAL MEETING OF SBBQ, 38., 2009, Águas de Lindóia. Program and index. [S.l.]: The Brazilian Biochemistry and Molecular Biology Society, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 39. | | RODRIGUES, L. P.; SOUTO, B. de M.; RAMOS, T. G. S.; FRANCO, O. L.; QUIRINO, B. F. Screening of a moxotó goat rumen small insert metagenomic library for cellulosic activity for biotechnological use. In: REUNIÃO ANUAL DA SBBQ, 42., 2013, Foz do Iguaçu, PR. [Anais...] São Paulo: SBBq, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
| |
| 40. | | LIMA, T. B.; SILVA, O. N.; SCALABRIN, F.; SILVA, L. P. da; ROCHA, T. L.; CASTRO, C. F. de S.; FRANCO, O. L.; GROSI DE SA, M. F. Avaliação da atividade laxativa dos extratos das folhas e frutos de cagaita (Eugenia Dysenterica, DC). In: CONGRESSO NACIONAL DE BOTÂNICA, 58., 2007, São Paulo. A botânica no Brasil: pesquisa, ensino e políticas públicas ambientais. [S.l.]: Sociedade Botânica do Brasil, 2007.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| Registros recuperados : 157 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
