| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
14/11/1997 |
|
Data da última atualização: |
11/06/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FONTES, E. P. B.; SILVA, C. J.; CAROLINO, S. M. B.; FIGUEREDO, J. D. F.; BATISTA, D. P. O. |
|
Afiliação: |
EMBRAPA/CNPMS. |
|
Título: |
A soybean binding protein (BiP) homolog is temporally regulated in soybean seeds and associates detectably with normal storage proteins in vitro. |
|
Ano de publicação: |
1996 |
|
Fonte/Imprenta: |
Brazilian Journal of Genetics, Ribeirão Preto, v. 19, n. 2, p. 305-312, 1996. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The endoplasmic reticulum (ER) luminal binding protein (BiP) is thought to be a key mediator of folding and assembly of de novo synthesized secretory proteins. We have used a maize (Zea mays L.) BiP antibady to identify its homolog in soybeans (Glycine max (L.) Merril). The accumulation of BiP in developing soybean seeds seems to be coordinated with the onset of active storage protein synthesis. We used a co-immunoprecipitation assay to detect soybean BiP:B-conglycinin interactions. Either a maize BiP antibody or a-B-conglycinin antibody co-immunoprecipitated the reciprocal protein from whole seed protein extract enzymatically depleted of adenosine 5-triphosphate (ATP), while an unrelated antibody failed to immunoprecipitate either one. The association ofBiP:B-conglycinin complexes was completely reversed by addition of ATP, a diagnostic feature of molecular chaperone-mediated interaction. However, only a very small fraction of B-conglycinin was found to be associated with BiP in whole cell protein extracts from immature seeds. These results are consistent with a transient association between BiP and B-conglycinin subunits, and suggests its involvement in the biosynthetic transport pathway of storage proteins to protein bodies. |
|
Palavras-Chave: |
Protein; Seed; Soybean. |
|
Thesagro: |
Biologia Molecular; Glycine Max; Proteína; Semente; Soja. |
|
Thesaurus Nal: |
molecular biology. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/38228/1/Soybean-binding.pdf
|
|
Marc: |
LEADER 02091naa a2200277 a 4500
001 1477302
005 2018-06-11
008 1996 bl uuuu u00u1 u #d
100 1 $aFONTES, E. P. B.
245 $aA soybean binding protein (BiP) homolog is temporally regulated in soybean seeds and associates detectably with normal storage proteins in vitro.$h[electronic resource]
260 $c1996
520 $aThe endoplasmic reticulum (ER) luminal binding protein (BiP) is thought to be a key mediator of folding and assembly of de novo synthesized secretory proteins. We have used a maize (Zea mays L.) BiP antibady to identify its homolog in soybeans (Glycine max (L.) Merril). The accumulation of BiP in developing soybean seeds seems to be coordinated with the onset of active storage protein synthesis. We used a co-immunoprecipitation assay to detect soybean BiP:B-conglycinin interactions. Either a maize BiP antibody or a-B-conglycinin antibody co-immunoprecipitated the reciprocal protein from whole seed protein extract enzymatically depleted of adenosine 5-triphosphate (ATP), while an unrelated antibody failed to immunoprecipitate either one. The association ofBiP:B-conglycinin complexes was completely reversed by addition of ATP, a diagnostic feature of molecular chaperone-mediated interaction. However, only a very small fraction of B-conglycinin was found to be associated with BiP in whole cell protein extracts from immature seeds. These results are consistent with a transient association between BiP and B-conglycinin subunits, and suggests its involvement in the biosynthetic transport pathway of storage proteins to protein bodies.
650 $amolecular biology
650 $aBiologia Molecular
650 $aGlycine Max
650 $aProteína
650 $aSemente
650 $aSoja
653 $aProtein
653 $aSeed
653 $aSoybean
700 1 $aSILVA, C. J.
700 1 $aCAROLINO, S. M. B.
700 1 $aFIGUEREDO, J. D. F.
700 1 $aBATISTA, D. P. O.
773 $tBrazilian Journal of Genetics, Ribeirão Preto$gv. 19, n. 2, p. 305-312, 1996.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Solos. |
|
Data corrente: |
15/01/2010 |
|
Data da última atualização: |
15/01/2010 |
|
Tipo da produção científica: |
Monitoramento/Zoneamento |
|
Autoria: |
AMARAL, F. C. S. do; CARVALHO JUNIOR, W. de; BHERING, S. B.; CHAGAS, C. da S.; ÁGLIO, M. L. D. |
|
Afiliação: |
FERNANDO CEZAR SARAIVA DO AMARAL, CNPS; WALDIR DE CARVALHO JUNIOR, CNPS; SILVIO BARGE BHERING, CNPS; CESAR DA SILVA CHAGAS, CNPS; MARIO LUIZ DIAMANTE ÁGLIO, ASSISTENTE DE PESQUISA CNPS. |
|
Título: |
Mapa de classes de terras para irrigação por superfície cultura da cana projeto Jequitaí - MG. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
In: AMARAL, F. C. S. do; CARVALHO JUNIOR, W. de; BHERING, S. B.; CHAGAS, C. da S.; ÁGLIO, M. L. D. Classificação das Terras para Irrigação - SiBCTI. Rio de janeiro: Embrapa Solos; Brasília: CODEVASF, 2009. 68 p. |
|
Páginas: |
1 p. |
|
Descrição Física: |
1 mapa color. escala 1:50.000. |
|
Idioma: |
Português |
|
Thesagro: |
Solo. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00723nem a2200169 a 4500
001 1580612
005 2010-01-15
008 2009 bl uuuu u0uu1 u #d
100 1 $aAMARAL, F. C. S. do
245 $aMapa de classes de terras para irrigação por superfície cultura da cana projeto Jequitaí - MG.
260 $aIn: AMARAL, F. C. S. do; CARVALHO JUNIOR, W. de; BHERING, S. B.; CHAGAS, C. da S.; ÁGLIO, M. L. D. Classificação das Terras para Irrigação - SiBCTI. Rio de janeiro: Embrapa Solos; Brasília: CODEVASF, 2009. 68 p.$c2009
300 $a1 p.$c1 mapa color. escala 1:50.000.
650 $aSolo
700 1 $aCARVALHO JUNIOR, W. de
700 1 $aBHERING, S. B.
700 1 $aCHAGAS, C. da S.
700 1 $aÁGLIO, M. L. D.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Solos (CNPS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
