|
|
Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
14/01/2011 |
Data da última atualização: |
17/04/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, OSMUNDO B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. |
Afiliação: |
ÉRICO A. R. VASCONCELOS, UNB; CELSO G. SANTANA; CLAUDIA VIEIRA GODOY, CNPSO; MARILIA SANTOS SILVA, CPAC; LEONORA R. S. MOREIRA, UNB; OSMUNDO B. OLIVEIRA-NETO; DANIEL PRICE, Durham University; ELAINE FITCHES, Durham University; EDIVALDO X. F. FILHO, UNB; ANGELA MEHTA DOS REIS, CENARGEN; JOHN A. GATEHOUSE, Durham University; MARIA FATIMA GROSSI DE SA, CENARGEN. |
Título: |
A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec, 2010. |
Idioma: |
Inglês |
Conteúdo: |
Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control. |
Palavras-Chave: |
Asian rust. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31109/1/cbb.errata.cvg.pdf
|
Marc: |
LEADER 02303nam a2200253 a 4500 001 1873147 005 2018-04-17 008 2010 bl uuuu u00u1 u #d 100 1 $aVASCONCELOS, E. A. R. 245 $aA new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. 260 $aIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec$c2010 520 $aAsian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control. 653 $aAsian rust 700 1 $aSANTANA, C. G. 700 1 $aGODOY, C. V. 700 1 $aSILVA, M. S. 700 1 $aMOREIRA, L. R. S. 700 1 $aOLIVEIRA-NETO, OSMUNDO B. 700 1 $aPRICE, D. 700 1 $aFITCHES, E. 700 1 $aFILHO, E. X. F. 700 1 $aMEHTA, A. 700 1 $aGATEHOUSE, J. A. 700 1 $aGROSSI-DE-SA, M. F.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
14/02/2019 |
Data da última atualização: |
08/05/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ABREU, C. A.; RAIJ, B. van; GABE, U.; ABREU, M. F.; PAZ-GONZALEZ, A. |
Afiliação: |
CLEIDE A. ABREU, Instituto Agronômico/Campinas; BERNARDO van RAIJ, Embrapa Meio Ambiente; URSULA GABE, Instituto Biológico/Campinas; MONICA F. ABREU, Instituto Biológico/Campinas; ANTONIO PAZ-GONZALEZ, Universidade da Coruña/Spain. |
Título: |
Efficiency of multinutrient extractants for the determining of available zinc in soils. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Communications in Soil Science and Plant Analysis, V. 33, n. 15-18, p. 3313-3324, 2002. |
Idioma: |
Inglês |
Palavras-Chave: |
Multinutrient. |
Thesagro: |
Análise do Solo; Zinco. |
Thesaurus NAL: |
Agricultural soils. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00639naa a2200205 a 4500 001 2106026 005 2019-05-08 008 2002 bl uuuu u00u1 u #d 100 1 $aABREU, C. A. 245 $aEfficiency of multinutrient extractants for the determining of available zinc in soils.$h[electronic resource] 260 $c2002 650 $aAgricultural soils 650 $aAnálise do Solo 650 $aZinco 653 $aMultinutrient 700 1 $aRAIJ, B. van 700 1 $aGABE, U. 700 1 $aABREU, M. F. 700 1 $aPAZ-GONZALEZ, A. 773 $tCommunications in Soil Science and Plant Analysis, V. 33$gn. 15-18, p. 3313-3324, 2002.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|