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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
18/11/2019 |
Data da última atualização: |
11/09/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CHAIBUB, A. A.; SOUSA, T. P. de; ARAÚJO, L. G. de; FILIPPI, M. C. C. de. |
Afiliação: |
AMANDA ABDALLAH CHAIBUB, UNB; THATYANE PEREIRA DE SOUSA, UFG; LEILA GARCÊS DE ARAÚJO, UFG; MARTA CRISTINA CORSI DE FILIPPI, CNPAF. |
Título: |
Cladosporium cladosporioides C24G modulates gene expression and enzymatic activity during leaf blast suppression in rice plants. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Journal of Plant Growth Regulation, v. 39, n. 3, p. 1140-1152, Sept. 2020. |
Série: |
0721-7595 |
ISSN: |
1435-8107 |
DOI: |
https://doi.org/10.1007/s00344-019-10052-9 |
Idioma: |
Inglês |
Conteúdo: |
The inclusion of biological control in the integrated management of rice blast (Magnaporthe oryzae) is an alternative to reduce pesticides application. C24G, classified, as Cladosporium cladosporioides was isolated from the phylloplane of the rice plant, therefore, adapted to natural conditions of the original habitat. This study aimed to compare four application methods of C24G in rice plants to suppress leaf blast together with the increase in enzymatic activity and expression of defense genes. It was conducted by four assays (1: seed and soil, 2: soil drenching, 3: foliar spray pulverization-preventive and 4: foliar spray pulverization-curative) for choosing the best application method. The best methods identified were further investigated for the activity of Chitinase (CHI), B-1,3-Glucanase (GLU), Lipoxygenase (LOX), Phenylalanine ammonia-lyase (PAL), and Peroxidase (POX) and the expression of Gns1, JIOsPR10, LOX-RLL, and PR1b genes by Real-time PCR. The preventive foliar spray pulverization suppressed up to 83.78% of leaf blast severity, increasing enzymes (CHI, GLU, LOX, and PAL) activity and genes (JIOsPR10, LOX-RLL, and PR1b) expression. We conclude that Cladosporium cladosporioides isolated C24G is a potential biological agent. To prove its potential as a component of sustainable blast management, it should be tested under field conditions. The application of C24G isolate in rice fields can reduce the number of fungicides spraying, generating greater rentability and decreasing environmental contaminations. MenosThe inclusion of biological control in the integrated management of rice blast (Magnaporthe oryzae) is an alternative to reduce pesticides application. C24G, classified, as Cladosporium cladosporioides was isolated from the phylloplane of the rice plant, therefore, adapted to natural conditions of the original habitat. This study aimed to compare four application methods of C24G in rice plants to suppress leaf blast together with the increase in enzymatic activity and expression of defense genes. It was conducted by four assays (1: seed and soil, 2: soil drenching, 3: foliar spray pulverization-preventive and 4: foliar spray pulverization-curative) for choosing the best application method. The best methods identified were further investigated for the activity of Chitinase (CHI), B-1,3-Glucanase (GLU), Lipoxygenase (LOX), Phenylalanine ammonia-lyase (PAL), and Peroxidase (POX) and the expression of Gns1, JIOsPR10, LOX-RLL, and PR1b genes by Real-time PCR. The preventive foliar spray pulverization suppressed up to 83.78% of leaf blast severity, increasing enzymes (CHI, GLU, LOX, and PAL) activity and genes (JIOsPR10, LOX-RLL, and PR1b) expression. We conclude that Cladosporium cladosporioides isolated C24G is a potential biological agent. To prove its potential as a component of sustainable blast management, it should be tested under field conditions. The application of C24G isolate in rice fields can reduce the number of fungicides spraying, generating greater rentability and... Mostrar Tudo |
Thesagro: |
Arroz; Atividade Enzimática; Mancha de Cladosporium; Oryza Sativa. |
Thesaurus Nal: |
Blast disease; Cladosporium cladosporioides; Induced resistance; Magnaporthe oryzae; Rice. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02503naa a2200301 a 4500 001 2114465 005 2020-09-11 008 2020 bl uuuu u00u1 u #d 022 $a1435-8107 024 7 $ahttps://doi.org/10.1007/s00344-019-10052-9$2DOI 100 1 $aCHAIBUB, A. A. 245 $aCladosporium cladosporioides C24G modulates gene expression and enzymatic activity during leaf blast suppression in rice plants.$h[electronic resource] 260 $c2020 490 $a0721-7595 520 $aThe inclusion of biological control in the integrated management of rice blast (Magnaporthe oryzae) is an alternative to reduce pesticides application. C24G, classified, as Cladosporium cladosporioides was isolated from the phylloplane of the rice plant, therefore, adapted to natural conditions of the original habitat. This study aimed to compare four application methods of C24G in rice plants to suppress leaf blast together with the increase in enzymatic activity and expression of defense genes. It was conducted by four assays (1: seed and soil, 2: soil drenching, 3: foliar spray pulverization-preventive and 4: foliar spray pulverization-curative) for choosing the best application method. The best methods identified were further investigated for the activity of Chitinase (CHI), B-1,3-Glucanase (GLU), Lipoxygenase (LOX), Phenylalanine ammonia-lyase (PAL), and Peroxidase (POX) and the expression of Gns1, JIOsPR10, LOX-RLL, and PR1b genes by Real-time PCR. The preventive foliar spray pulverization suppressed up to 83.78% of leaf blast severity, increasing enzymes (CHI, GLU, LOX, and PAL) activity and genes (JIOsPR10, LOX-RLL, and PR1b) expression. We conclude that Cladosporium cladosporioides isolated C24G is a potential biological agent. To prove its potential as a component of sustainable blast management, it should be tested under field conditions. The application of C24G isolate in rice fields can reduce the number of fungicides spraying, generating greater rentability and decreasing environmental contaminations. 650 $aBlast disease 650 $aCladosporium cladosporioides 650 $aInduced resistance 650 $aMagnaporthe oryzae 650 $aRice 650 $aArroz 650 $aAtividade Enzimática 650 $aMancha de Cladosporium 650 $aOryza Sativa 700 1 $aSOUSA, T. P. de 700 1 $aARAÚJO, L. G. de 700 1 $aFILIPPI, M. C. C. de 773 $tJournal of Plant Growth Regulation$gv. 39, n. 3, p. 1140-1152, Sept. 2020.
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23. | | PEIXOTO, C. N.; OTTONI, G.; FILIPPI, M. C. C.; SILVA-LOBO, V. L.; PRABHU, A. S. Biology of Gaeumannomyces graminis var. graminis isolates from rice and grasses and epidemiological aspects of crown sheath rot of rice. Tropical Plant Pathology, Brasília, DF, v. 38, n. 6, p. 495-504, nov./dez. 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Arroz e Feijão. |
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36. | | LOBO, V. L. da S.; FILIPPI, M. C. C. de; PRABHU, A. S. Doenças. In: SANTIAGO, C. M.; BRESEGHELLO, H. C. de P.; FERREIRA, C. M. (Ed.). Arroz: o produtor pergunta, a Embrapa responde. 2. ed. rev. e ampl. Brasília, DF: Embrapa, 2013. p. 143-158. (Coleção 500 perguntas, 500 respostas).Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Arroz e Feijão. |
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Registros recuperados : 329 | |
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